Akinade_2022_Sci.Rep_12_4594

Reference

Title : Protective capacity of carotenoid trans-astaxanthin in rotenone-induced toxicity in Drosophila melanogaster - Akinade_2022_Sci.Rep_12_4594
Author(s) : Akinade TC , Babatunde OO , Adedara AO , Adeyemi OE , Otenaike TA , Ashaolu OP , Johnson TO , Terriente-Felix A , Whitworth AJ , Abolaji AO
Ref : Sci Rep , 12 :4594 , 2022
Abstract :

Trans-astaxanthin (TA), a keto-carotenoid found in aquatic invertebrates, possesses anti-oxidative and anti-inflammatory activities. Rotenone is used to induce oxidative stress-mediated Parkinson's disease (PD) in animals. We probed if TA would protect against rotenone-induced toxicity in Drosophila melanogaster. Trans-astaxanthin (0, 0.1, 0.5, 1.0, 2.5, 10, and 20 mg/10 g diet) and rotenone (0, 250 and 500 microM) were separately orally exposed to flies in the diet to evaluate longevity and survival rates, respectively. Consequently, we evaluated the ameliorative actions of TA (1.0 mg/10 g diet) on rotenone (500 microM)-induced toxicity in Drosophila after 7 days' exposure. Additionally, we performed molecular docking of TA against selected pro-inflammatory protein targets. We observed that TA (0.5 and 1.0 mg/10 g diet) increased the lifespan of D. melanogaster by 36.36%. Moreover, TA (1.0 mg/10 g diet) ameliorated rotenone-mediated inhibition of Catalase, Glutathione-S-transferase and Acetylcholinesterase activities, and depletion of Total Thiols and Non-Protein Thiols contents. Trans-astaxanthin prevented behavioural dysfunction and accumulation of Hydrogen Peroxide, Malondialdehyde, Protein Carbonyls and Nitric Oxide in D. melanogaster (p < 0.05). Trans-astaxanthin showed higher docking scores against the pro-inflammatory protein targets evaluated than the standard inhibitors. Conclusively, the structural features of TA might have contributed to its protective actions against rotenone-induced toxicity.

PubMedSearch : Akinade_2022_Sci.Rep_12_4594
PubMedID: 35301354

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Citations formats

Akinade TC, Babatunde OO, Adedara AO, Adeyemi OE, Otenaike TA, Ashaolu OP, Johnson TO, Terriente-Felix A, Whitworth AJ, Abolaji AO (2022)
Protective capacity of carotenoid trans-astaxanthin in rotenone-induced toxicity in Drosophila melanogaster
Sci Rep 12 :4594

Akinade TC, Babatunde OO, Adedara AO, Adeyemi OE, Otenaike TA, Ashaolu OP, Johnson TO, Terriente-Felix A, Whitworth AJ, Abolaji AO (2022)
Sci Rep 12 :4594