| Title : Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation - Angkawidjaja_2007_FEBS.Lett_581_5060 |
| Author(s) : Angkawidjaja C , You DJ , Matsumura H , Kuwahara K , Koga Y , Takano K , Kanaya S |
| Ref : FEBS Letters , 581 :5060 , 2007 |
|
Abstract :
The crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation was determined at 1.5A resolution. This structure highly resembles that of Serratia marcescens LipA in an open conformation, except for the structures of two lids. Lid1 is anchored by a Ca2+ ion (Ca1) in an open conformation, but lacks this Ca1 site and greatly changes its structure and position in a closed conformation. Lid2 forms a helical hairpin in an open conformation, but does not form it and covers the active site in a closed conformation. Based on these results, we discuss on the lid-opening mechanism. |
| PubMedSearch : Angkawidjaja_2007_FEBS.Lett_581_5060 |
| PubMedID: 17923123 |
| Gene_locus related to this paper: psesp-Q9RBY1 |
| Gene_locus | psesp-Q9RBY1 |
| Family | Bacterial_lipase Bacterial_lip_FamI.3 |
| Structure | 2Z8X 2Z8Z |
Angkawidjaja C, You DJ, Matsumura H, Kuwahara K, Koga Y, Takano K, Kanaya S (2007)
Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation
FEBS Letters
581 :5060
Angkawidjaja C, You DJ, Matsumura H, Kuwahara K, Koga Y, Takano K, Kanaya S (2007)
FEBS Letters
581 :5060