Bacterial_lip_FamI.3

Relationship

Family: Bacterial_lip_FamI.3

Block: L

Parent Family: Bacterial_lipase

Comment

This family corresponds to family I.3 of the classification of Arpigny and Jaeger (1999) The N-catalytic domain (residues 1-370) contains the active site residues, Ser207, Asp255, and His313 4, 5. The C-domain contains several repeats of the RTX motif and a putative secretion signal near the C-terminus. The C-domain contains two beta-roll motifs, laterally stacked together forming the so called beta-roll sandwich. The first beta-roll motif consists of residues 373-417, containing five RTX repeats and binds three Ca2+ ions. The second beta-roll motif consists of residues 493-568, containing eight RTX repeats and binds five Ca2+ ions. HemolysinCabind (PF00353). This family contains Polyurethanases (PURase)

Database

Interpro : No interpro

PIRSF : No PIRSF

Pdoc : No Pdoc

Pfam : No Pfam

Prints : No Print

EC Number : No EC Number

Sequences

Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
2ZVD Crystal structure of Pseudomonas sp. MIS38 lipase in an open conformation psesp-Q9RBY1
2ZJ6 Crystal structure of D337A mutant of Pseudomonas sp. MIS38 lipase psesp-Q9RBY1
2ZJ7 Crystal structure of D157A mutant of Pseudomonas sp. MIS38 lipase psesp-Q9RBY1
3A6Z Crystal structure of Pseudomonas sp. MIS38 lipase (PML) in the open conformation following dialysis against Ca-free buffer psesp-Q9RBY1
3A70 Crystal structure of Pseudomonas sp. MIS38 lipase in complex with diethyl phosphate psesp-Q9RBY1
2Z8X Crystal structure of extracellular lipase from Pseudomonas sp. MIS38 (CASP Target) psesp-Q9RBY1
2Z8Z Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase psesp-Q9RBY1
2QUA Crystal structure of extracellular lipase LipA from Serratia marcescens Space Group P321 serma-lipasA
2QUB Crystal structure of extracellular lipase LipA from Serratia marcescens Space Group H3 serma-lipasA

Gene_locus Name Species
9sped-a0a0s2cee2 Candidatus Pseudomonas adelgestsugas\; Pseudomonas sp. strain TB11 esterase Candidatus Pseudomonas adelgestsugas
psefs-c3kbe9 Pseudomonas fluorescens (strain SBW25)lipB Thermostable and Organic Solution-Tolerant Lipase Pseudomonas fluorescens SBW25
9bact-g9hwf0 uncultured bacterium Lipase lipB SMlipB uncultured bacterium
psesp-PURase Pseudomonas sp Polyurethanase (PURase) Pseudomonas sp.
serli-a0a109z2v1 Serratia liquefaciens polyurethanase Serratia liquefaciens
9psed-LipYY31 Pseudomonas sp. strain YY31 solvent-tolerant lipase from a psychrotrophic bacterium Pseudomonas sp. YY31
9bact-q6xci1 uncultured bacterium lipb uncultured bacterium
9bact-q6xci2 uncultured bacterium lipa uncultured bacterium
9psed-q2ktb3 Pseudomonas sp. 7323 cold-active lipase (EC 3.1.1.3) Pseudomonas sp. 7323
9psed-q6vef5 Pseudomonas sp. JZ-2003 lipase Pseudomonas sp. JZ-2003
9psed-q66wt1 uncultured Pseudomonas sp lipase lipJ03 uncultured Pseudomonas sp.
pholl-q7n4l7 Photorhabdus luminescens (subsp. laumondii) similar to lipase Photorhabdus luminescens
psebr-lipa Pseudomonas brassicacearum lipa Pseudomonas brassicacearum
psech-PUEA Pseudomonas chlororaphis (Pseudomonas aureofaciens) polyurethanase esterase a PUEA Pseudomonas chlororaphis
psech-PUEB Pseudomonas chlororaphis (Pseudomonas aureofaciens) polyurethanase esterase b PUEB Pseudomonas chlororaphis
psef5-q4kbs3 Pseudomonas protegens (strain Pf-5 \/ ATCC BAA-477) polyurethanase B PUEB and secreted hemolysin-type EhxA RTX family calcium-binding bacteriocin, putative Pseudomonas fluorescens
psef5-q4kbs6 Pseudomonas protegens (strain Pf-5 \/ ATCC BAA-477) polyurethanase A PUEA and secreted hemolysin-type RTX family calcium-binding bacteriocin Pseudomonas fluorescens
psefl-LIPAPF33 Pseudomonas fluorescens\; Pseudomonas sp. AMS8 lipase LipAMS8, extracellular lipase Pseudomonas fluorescens
psefl-lipb Pseudomonas fluorescens lipase Pseudomonas fluorescens
psefl-LIPB2 Pseudomonas fluorescens lipase Pseudomonas fluorescens
psefl-PULA Pseudomonas fluorescens polyurethanase lipase A Pseudomonas fluorescens
psefl-q6gx93 Pseudomonas fluorescens ( and strain PfO-1) lipase Pseudomonas fluorescens
psefl-q7wzt7 Pseudomonas fluorescens lipase lipJ02 Pseudomonas fluorescens
psefl-q76d31 Pseudomonas fluorescens lipase (EC 3.1.1.3) Pseudomonas fluorescens
psefl-q670w0 Pseudomonas fluorescens lipase Pseudomonas fluorescens
psefl-siklip Pseudomonas fluorescens gene for triacylglycerol lipase, complete cds Pseudomonas fluorescens
psefl-TLIA Pseudomonas fluorescens thermostable lipase tlia Pseudomonas fluorescens
psepf-q3kcs9 Pseudomonas fluorescens (strain PfO-1) triacylglycerol lipase (EC 3.1.1.3) Pseudomonas fluorescens
psesp-KBLIP Pseudomonas sp. KB700A lipase Pseudomonas sp. KB700A
psesp-LIPUB48 Pseudomonas sp. UB48 lipase Pseudomonas sp. UB48
psesp-Q9RBY1 Pseudomonas sp. MIS38 lipase Pseudomonas sp. MIS38
psesp-Q52515 Pseudomonas sp lipase Pseudomonas sp.
serma-lipasA Serratia marcescens LipA gene for lipase Serratia marcescens
psywf-a5wgv1 Psychrobacter sp. (strain PRwf-1) Triacylglycerol lipase Psychrobacter sp. PRwf-1

Substrate Chemical Nomenclature Proteins
Ethyl-Zaltoprofen ethyl 2-(6-oxo-5H-benzo[b][1]benzothiepin-3-yl)propanoate 9zzzz-Est924    serma-lipasA
Ethyl-Flurbiprofen ethyl 2-(3-fluoro-4-phenylphenyl)propanoate 9zzzz-Est924    serma-lipasA    canar-LipB
Impranil 9flao-a0a330mq60    9flao-a0a0c1f4u8    9flao-kjj39608    9flao-a0a1m6f5v0    psefl-PULA    serli-a0a109z2v1    9pezi-CpCut1    9gamm-a0a1i6bky1
Polyurethane comac-polest    thefu-q6a0i4    thecd-d1a9g5    thecd-d1a2h1    psech-PUEA    psech-PUEB    psefl-PULA    psef5-q4kbs6    psef5-q4kbs3    psesp-PURase    9pezi-CpCut1
Ketoprofen-Ethyl-Ester ethyl 2-(3-benzoylphenyl)propanoate 9baci-d1mg60    9bact-q4tzq3    serma-lipasA    bacsu-lip    canar-LipB    9zzzz-Est924
Tributyrin 2,3-di(butanoyloxy)propyl butanoate bovin-balip    rabit-1cxes    pseae-llipa    canar-LipB    human-PNLIPRP2    talth-f6lqk7    nech7-c7yuz1    cavpo-2plrp    human-CEL    human-PNLIP    canan-lipasA    rhimi-lipas    rhidl-lipas    psesp-Q9RBY1    staha-Q9RGZ6    9bact-6EB3
Trioctanoin 2,3-di(octanoyloxy)propyl octanoate pseae-llipa    human-PNLIP    psesp-Q9RBY1
Trihexanoin 2,3-di(hexanoyloxy)propyl hexanoate bactc-lipas    pig-1plip    canar-LipB    humla-1lipa    thete-TTE1809    psesp-Q9RBY1

Inhibitor Chemical Nomenclature Proteins
Paraoxon diethyl (4-nitrophenyl) phosphate human-PLA2G7    human-PNLIPRP2    humin-cut    human-ACHE    promi-c2lfd0    yeast-yjg8    bacpu-AXE    sulto-ST0071    aspor-cutas    pig-1plip    fusso-cutas    lacrh-B2CZF3    thema-TM0033    colgl-cutas    psesp-Q9RBY1    actde-CXE1    myctu-a85c    thema-TM0077    rhimi-lipas    bacpu-w8fke7    9bact-KP212148

References (7)

Title : Enhanced enzyme thermostability of a family I.3 lipase LipSR1 by T118A mutation at the calcium-binding site - Jiang_2023_Biotechnol.Lett__
Author(s) : Jiang S , Zhou Z , Han J , Fan Q , Long Z , Wang J
Ref : Biotechnol Lett , : , 2023
Abstract :
PubMedSearch : Jiang_2023_Biotechnol.Lett__
PubMedID: 37439931
Gene_locus related to this paper: lacac-q5fi30

Title : X-ray crystallographic and MD simulation studies on the mechanism of interfacial activation of a family I.3 lipase with two lids - Angkawidjaja_2010_J.Mol.Biol_400_82
Author(s) : Angkawidjaja C , Matsumura H , Koga Y , Takano K , Kanaya S
Ref : Journal of Molecular Biology , 400 :82 , 2010
Abstract :
PubMedSearch : Angkawidjaja_2010_J.Mol.Biol_400_82
PubMedID: 20438738
Gene_locus related to this paper: psesp-Q9RBY1

Title : Importance of the Ca2+-binding sites in the N-catalytic domain of a family I.3 lipase for activity and stability - Kuwahara_2008_Protein.Eng.Des.Sel_21_737
Author(s) : Kuwahara K , Angkawidjaja C , Matsumura H , Koga Y , Takano K , Kanaya S
Ref : Protein Engineering Des Sel , 21 :737 , 2008
Abstract :
PubMedSearch : Kuwahara_2008_Protein.Eng.Des.Sel_21_737
PubMedID: 18987131
Gene_locus related to this paper: psesp-Q9RBY1

Title : A calcium-gated lid and a large beta-roll sandwich are revealed by the crystal structure of extracellular lipase from Serratia marcescens - Meier_2007_J.Biol.Chem_282_31477
Author(s) : Meier R , Drepper T , Svensson V , Jaeger KE , Baumann U
Ref : Journal of Biological Chemistry , 282 :31477 , 2007
Abstract :
PubMedSearch : Meier_2007_J.Biol.Chem_282_31477
PubMedID: 17728256
Gene_locus related to this paper: serma-lipasA

Title : Crystal structure of a family I.3 lipase from Pseudomonas sp. MIS38 in a closed conformation - Angkawidjaja_2007_FEBS.Lett_581_5060
Author(s) : Angkawidjaja C , You DJ , Matsumura H , Kuwahara K , Koga Y , Takano K , Kanaya S
Ref : FEBS Letters , 581 :5060 , 2007
Abstract :
PubMedSearch : Angkawidjaja_2007_FEBS.Lett_581_5060
PubMedID: 17923123
Gene_locus related to this paper: psesp-Q9RBY1

Title : Lipases for biotechnology - Jaeger_2002_Curr.Opin.Biotechnol_13_390
Author(s) : Jaeger KE , Eggert T
Ref : Curr Opin Biotechnol , 13 :390 , 2002
Abstract :
PubMedSearch : Jaeger_2002_Curr.Opin.Biotechnol_13_390
PubMedID: 12323363

Title : Bacterial lipolytic enzymes: classification and properties - Arpigny_1999_Biochem.J_343_177
Author(s) : Arpigny JL , Jaeger KE
Ref : Biochemical Journal , 343 :177 , 1999
Abstract :
PubMedSearch : Arpigny_1999_Biochem.J_343_177
PubMedID: 10493927