Angkawidjaja_2012_Febs.J_279_3071

Reference

Title : Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii. - Angkawidjaja_2012_Febs.J_279_3071
Author(s) : Angkawidjaja C , Koga Y , Takano K , Kanaya S
Ref : Febs J , 279 :3071 , 2012
Abstract : Hormone sensitive lipase (HSL) family is a family of carboxylesterases and lipases with similarity to mammalian HSL. Thermophilic enzymes of this family have high potential for the use in biocatalysis. We prepared and crystallized a carboxylesterase of HSL family from Sulfolobus tokodaii (Sto-Est), and determined its structures in the presence and absence of an inhibitor. Sto-Est forms a dimer in solution and the crystal structure suggests the presence of a stable biological dimer. We identified a residue close to the dimer interface, R267, which is conserved in the archaeal enzymes of HSL family and is in close proximity with the same residue from the other monomer. Mutations of R267 to Glu, Gly and Lys were conducted and the resultant R267 mutants were characterized and crystallized. The structures of R267E, R267G and R267K are highly similar to that of Sto-Est with only slight differences in atomic coordinates. The dimerized state of R267E and R267G are unstable under denaturing condition or at high temperature, as shown by urea-induced dimer dissociation experiment and MD simulation. R267E is the most unstable mutant protein, followed by R267G and R267K, as shown by the thermal denaturation curve and optimum temperature for activity. From the data we discuss the importance of R267 in maintaining the dimer integrity of Sto-Est.
ESTHER : Angkawidjaja_2012_Febs.J_279_3071
PubMedSearch : Angkawidjaja_2012_Febs.J_279_3071
PubMedID: 22748144
Gene_locus related to this paper: sulto-ST0071

Related information

Gene_locus related to this paper: sulto-ST0071

Citations formats

Angkawidjaja C, Koga Y, Takano K, Kanaya S (2012)
Structure and stability of a thermostable carboxylesterase from the thermoacidophilic archaeon Sulfolobus tokodaii.
Febs J 279 :3071

Angkawidjaja C, Koga Y, Takano K, Kanaya S (2012)
Febs J 279 :3071