Archer_2011_BMC.Genomics_12_9

Reference

Title : The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli - Archer_2011_BMC.Genomics_12_9
Author(s) : Archer CT , Kim JF , Jeong H , Park JH , Vickers CE , Lee SY , Nielsen LK
Ref : BMC Genomics , 12 :9 , 2011
Abstract :

BACKGROUND: Escherichia coli is a model prokaryote, an important pathogen, and a key organism for industrial biotechnology. E. coli W (ATCC 9637), one of four strains designated as safe for laboratory purposes, has not been sequenced. E. coli W is a fast-growing strain and is the only safe strain that can utilize sucrose as a carbon source. Lifecycle analysis has demonstrated that sucrose from sugarcane is a preferred carbon source for industrial bioprocesses.
RESULTS: We have sequenced and annotated the genome of E. coli W. The chromosome is 4,900,968 bp and encodes 4,764 ORFs. Two plasmids, pRK1 (102,536 bp) and pRK2 (5,360 bp), are also present. W has unique features relative to other sequenced laboratory strains (K-12, B and Crooks): it has a larger genome and belongs to phylogroup B1 rather than A. W also grows on a much broader range of carbon sources than does K-12. A genome-scale reconstruction was developed and validated in order to interrogate metabolic properties.
CONCLUSIONS: The genome of W is more similar to commensal and pathogenic B1 strains than phylogroup A strains, and therefore has greater utility for comparative analyses with these strains. W should therefore be the strain of choice, or 'type strain' for group B1 comparative analyses. The genome annotation and tools created here are expected to allow further utilization and development of E. coli W as an industrial organism for sucrose-based bioprocesses. Refinements in our E. coli metabolic reconstruction allow it to more accurately define E. coli metabolism relative to previous models.

PubMedSearch : Archer_2011_BMC.Genomics_12_9
PubMedID: 21208457
Gene_locus related to this paper: ecoli-entf , ecoli-fes , ecoli-yafa , ecoli-yeiG , ecoli-yhet , ecoli-yiel

Related information

Gene_locus ecoli-entf    ecoli-fes    ecoli-yafa    ecoli-yeiG    ecoli-yhet    ecoli-yiel

Citations formats

Archer CT, Kim JF, Jeong H, Park JH, Vickers CE, Lee SY, Nielsen LK (2011)
The genome sequence of E. coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli
BMC Genomics 12 :9

Archer CT, Kim JF, Jeong H, Park JH, Vickers CE, Lee SY, Nielsen LK (2011)
BMC Genomics 12 :9