Biundo_2016_Appl.Microbiol.Biotechnol_100_1753

Reference

Title : Characterization of a poly(butylene adipate-co-terephthalate)-hydrolyzing lipase from Pelosinus fermentans - Biundo_2016_Appl.Microbiol.Biotechnol_100_1753
Author(s) : Biundo A , Hromic A , Pavkov-Keller T , Gruber K , Quartinello F , Haernvall K , Perz V , Arrell MS , Zinn M , Ribitsch D , Guebitz GM
Ref : Applied Microbiology & Biotechnology , 100 :1753 , 2016
Abstract :

Certain alpha/beta hydrolases have the ability to hydrolyze synthetic polyesters. While their partial hydrolysis has a potential for surface functionalization, complete hydrolysis allows recycling of valuable building blocks. Although knowledge about biodegradation of these materials is important regarding their fate in the environment, it is currently limited to aerobic organisms. A lipase from the anaerobic groundwater organism Pelosinus fermentans DSM 17108 (PfL1) was cloned and expressed in Escherichia coli BL21-Gold(DE3) and purified from the cell extract. Biochemical characterization with small substrates showed thermoalkalophilic properties (T opt = 50 degrees C, pHopt = 7.5) and higher activity towards para-nitrophenyl octanoate (12.7 U mg-1) compared to longer and shorter chain lengths (C14 0.7 U mg-1 and C2 4.3 U mg-1, respectively). Crystallization and determination of the 3-D structure displayed the presence of a lid structure and a zinc ion surrounded by an extra domain. These properties classify the enzyme into the I.5 lipase family. PfL1 is able to hydrolyze poly(1,4-butylene adipate-co-terephthalate) (PBAT) polymeric substrates. The hydrolysis of PBAT showed the release of small building blocks as detected by liquid chromatography-mass spectrometry (LC-MS). Protein dynamics seem to be involved with lid opening for the hydrolysis of PBAT by PfL1.

PubMedSearch : Biundo_2016_Appl.Microbiol.Biotechnol_100_1753
PubMedID: 26490551
Gene_locus related to this paper: 9firm-a0a0a0ymq9

Citations formats

Biundo A, Hromic A, Pavkov-Keller T, Gruber K, Quartinello F, Haernvall K, Perz V, Arrell MS, Zinn M, Ribitsch D, Guebitz GM (2016)
Characterization of a poly(butylene adipate-co-terephthalate)-hydrolyzing lipase from Pelosinus fermentans
Applied Microbiology & Biotechnology 100 :1753

Biundo A, Hromic A, Pavkov-Keller T, Gruber K, Quartinello F, Haernvall K, Perz V, Arrell MS, Zinn M, Ribitsch D, Guebitz GM (2016)
Applied Microbiology & Biotechnology 100 :1753