Bacterial_lip_FamI.5
Relationship
Comment
This family correspond to family I.5 of the classification of Arpigny and Jaeger (1999) and abH15 of the LED database. It includes lipases from gram positive bacteria, organic-solvent tolerant, showing thermoalkalophilic properties and high molecular weight resulting from extra domain where a zinc ion is coordinatively bound to the enzyme. The family also contains poly (butylene adipate-co-terephthalate)-hydrolyzing lipase from Pelosinus fermentans
Database
Interpro : No interpro
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : No Pfam
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Structure | Name | Proteins |
|---|---|---|
| 5AH1 | Structure of EstA Cbotu_EstA from Clostridium botulinum | clobh-A51055 |
| 5CE5 | Probing the roles of two tryptophans surrounding the unique zinc coordination site in lipase family I.5 | bactc-lipas |
| 5AH0 | Biochemical and Structural Characterization of a PBAT-Hydrolyzing Lipase from Pelosinus fermentans | 9firm-a0a0a0ymq9 |
| 6S3G | Crystal Structure of lipase from Geobacillus stearothermophilus T6 variant A187C\/F291C | geose-q93a71 |
| 6S3J | Crystal Structure of lipase from Geobacillus stearothermophilus T6 variant E134C\/F149C | geose-q93a71 |
| 6S3V | Crystal Structure of lipase from Geobacillus stearothermophilus T6 variant E251C\/G332C | geose-q93a71 |
| 6FZ1 | Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant L360F | geose-q93a71 |
| 6FZ7 | Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant L184F | geose-q93a71 |
| 6FZ8 | Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant L184F\/A187F | geose-q93a71 |
| 6FZ9 | Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant A187F\/L360F | geose-q93a71 |
| 6FZA | Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant A187FL360F | geose-q93a71 |
| 6FZC | Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant L184F\/L360F | geose-q93a71 |
| 6FZD | Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant L184F\/A187F\/L360F | geose-q93a71 |
| 4X85 | Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant H86Y\/A269T\/R374W | geose-q93a71 |
| 4X6U | Crystal Structure of lipase from Geobacillus stearothermophilus T6 | geose-q93a71 |
| 4X71 | Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant A269T | geose-q93a71 |
| 4X7B | Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant H86Y\/A269T | geose-q93a71 |
| 4FMP | Crystal structure of thermostable, organic-solvent tolerant lipase from Geobacillus sp. strain ARM A0MTM1 | geose-q93a71 |
| 6A12 | X-ray structure of lipase from Geobacillus thermoleovorans | bacsp-lip |
| 5XPX | Structure elucidation of truncated AMS3 lipase from an Antarctic Pseudomonas | bacsp-lip |
| 4FKB | An Organic solvent tolerant lipase 42 | bacsp-lip |
| 4FDM | Crystallization and 3D structure elucidation of thermostable L2 lipase from thermophilic locally isolated Bacillus sp. L2. | bacsp-lip |
| 3UMJ | Crystal Structure of D311E Lipase | bacsp-lip |
| 3AUK | Crystal structure of a lipase from Geobacillus sp. SBS-4S | bacsp-lip |
| 2W22 | Activation Mechanism of Bacterial Thermoalkalophilic Lipases BTL2 | bactc-lipas |
| 7EY3 | Geobacillus zalihae double cysteine mutations in T1 lipase: disulfide bond improved structure rigidity and stability. | bacsp-lip |
| 7BUK | Geobacillus zalihae T1 lipase mutant - 5M (D43E\/T118N\/E226D\/E250L\/N304E) | bacsp-lip |
| 2DSN | Crystal structure of Geobacillus zalihae T1 lipase | bacsp-lip |
| 2Z5G | Crystal structure of Geobacillus zalihae T1 lipase F16L mutant | bacsp-lip |
| 1JI3 | Thermostable bacterial lipase from Bacillus sterarothermophilus | geost-lipas |
| 1KU0 | Crystal structure of the Bacillus stearothermophilus L1 lipase | geost-lipas |
| Gene_locus | Name | Species |
|---|---|---|
| 9baci-a0a2u8ee84 | Caldibacillus thermoamylovorans (Bacillus thermoamylovorans) Lipase 4_120 | Caldibacillus thermoamylovorans |
| 9bact-a4uz11 | uncultured bacterium PLA depolymerase plaM5 | uncultured bacterium |
| 9baci-c4t9i5 | Caldibacillus thermoamylovorans (Bacillus thermoamylovorans)\; Bacillus circulans, lipase | Caldibacillus thermoamylovorans |
| bacce-r8lxe1 | Bacillus cereus\; Bacillus thuringiensis\; Bacillus sp.\; Bacillus bombysepticus str. Wang\; Streptococcus pneumoniae Lipase | Bacillus cereus |
| 9baci-c3bkz5 | Bacillus pseudomycoides DSM 12442 Lipase | Bacillus pseudomycoides DSM 12442 |
| 9baci-a0a073k8c1 | Bacillus gaemokensis Lipase | Bacillus gaemokensis |
| aneth-d3xb96 | Aneurinibacillus thermoaerophilus thermostable and organic solvent-tolerant lipase | Aneurinibacillus thermoaerophilus |
| clobh-A5I3I2 | Clostridium botulinum Putative secreted lipase lipB EstB Cbotu_EstB | Clostridium botulinum |
| clobh-A51055 |  Clostridium botulinum Putative secreted lipase lipA EstA Cbotu_EstA |
Clostridium botulinum |
| 9bact-a4uz10 | uncultured bacterium PLA depolymerase pLA-M4 pLAM4 | uncultured bacterium |
| bacsp-a0a1c8y7c2 | Bacillus sp. JR3 esterase LipJ | Bacillus sp. |
| bacan-BA2607 | Bacillus anthracis, Bacillus thuringiensis, Bacillus cereus lipase, putative | Bacillus anthracis |
| bactc-lipas | Bacillus thermocatenulatus triacylglycerol lipase (BTL2) |
Bacillus thermocatenulatus |
| clote-CTC00947 | Clostridium tetani lipase (EC 3.1.1.3) | Clostridium tetani |
| deira-DR0334 | Deinococcus radiodurans lipase, putative | Deinococcus radiodurans |
| geost-lipas | Bacillus stearothermophilus Geobacillus stearothermophilus EH97 lipase gene, complete cds |
Geobacillus stearothermophilus |
| bacsp-lip | Geobacillus thermoleovorans Geobacillus zalihae T1 Bacillus sp. 42 organic solvent tolerant lipase, ARA, T1 Lipase GD-95, Antarctic Pseudomonas AMS3 lipase |
Geobacillus thermoleovorans |
| geose-q93a71 | Geobacillus stearothermophilus (Bacillus stearothermophilus) T6 lipase |
Geobacillus stearothermophilus |
| 9firm-a0a0a0ymq9 | Pelosinus fermentans DSM 17108. Lipase |
Pelosinus fermentans DSM 17108 |
| 9flao-a0a101cn99 | Flavobacteriaceae bacterium CRH. triacylglycerol lipase FBL | Flavobacteriaceae bacterium CRH |
| 9baci-a0a150my45 | Geobacillus sp. B4113_201601. triacylglycerol lipase | Geobacillus sp. B4113_201601 |
| 9entr-a0a807lk52 | Kosakonia cowanii JCM 10956 = DSM 18146. triacylglycerol lipase ECL | Kosakonia cowanii JCM 10956 = DSM 18146 |
| 9bacl-w2e7n6 | Paenibacillus larvae subsp. larvae triacylglycerol lipase PLL | Paenibacillus larvae subsp. larvae |
| Inhibitor | Chemical Nomenclature | Proteins |
|---|---|---|
| Lauramine-oxide | N,N-dimethyldodecan-1-amine oxide | 9eury-6QE2 bactc-lipas |
| 1-hexadecanesulfonyl-chloride | hexadecane-1-sulfonyl chloride | aliac-est2 canru-1lipa |
| PMSF | Phenylmethylsulfonyl fluoride | theet-q3ch51 emeni-q5avy9 human-ABHD10 human-PREPL myctu-Rv1430 psefl-cxest 9bact-E40 thefu-q6a0i4 ecoli-Aes orysj-Q10QA5 bac25-mglp 9bact-Q0GMU2 bacsu-FENB psepu-clcd1 thema-TM0077 bacsu-RsbQ ecoli-bioh drome-CG9542 human-FAP tubmm-TmEst2 human-NOTUM cutac-e6dae5 9gamm-urc15994 9baci-EstD9 geost-est30 pholl-AntI |
| Triton-X-100 | t-Octylphenoxypolyethoxyethanol \; 14-[4-(1,1,3,3-Tetramethylbutyl)phenoxy]-3,6,9,12-tetraoxatetradecan-1-ol | bactc-lipas tubmm-TmEst2 strhe-ShHTL7 |
References (11)
| Title : Characterization of a poly(butylene adipate-co-terephthalate)-hydrolyzing lipase from Pelosinus fermentans - Biundo_2016_Appl.Microbiol.Biotechnol_100_1753 |
| Author(s) : Biundo A , Hromic A , Pavkov-Keller T , Gruber K , Quartinello F , Haernvall K , Perz V , Arrell MS , Zinn M , Ribitsch D , Guebitz GM |
| Ref : Applied Microbiology & Biotechnology , 100 :1753 , 2016 |
| Abstract : |
| PubMedSearch : Biundo_2016_Appl.Microbiol.Biotechnol_100_1753 |
| PubMedID: 26490551 |
| Gene_locus related to this paper: 9firm-a0a0a0ymq9 |
| Title : Improvement of thermal stability via outer-loop ion pair interaction of mutated T1 lipase from Geobacillus zalihae strain T1 - Ruslan_2012_Int.J.Mol.Sci_13_943 |
| Author(s) : Ruslan R , Rahman RNZRA , Leow ATC , Ali MSM , Basri M , Salleh AB |
| Ref : Int J Mol Sci , 13 :943 , 2012 |
| Abstract : |
| PubMedSearch : Ruslan_2012_Int.J.Mol.Sci_13_943 |
| PubMedID: 22312296 |
| Gene_locus related to this paper: bacsp-lip |
| Title : High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM - Ebrahimpour_2011_Bioresour.Technol_102_6972 |
| Author(s) : Ebrahimpour A , Rahman RNZRA , Basri M , Salleh AB |
| Ref : Bioresour Technol , 102 :6972 , 2011 |
| Abstract : |
| PubMedSearch : Ebrahimpour_2011_Bioresour.Technol_102_6972 |
| PubMedID: 21531550 |
| Gene_locus related to this paper: bacsp-lip , geose-q93a71 |
| Title : Crystallization and preliminary X-ray crystallographic analysis of a thermostable organic solvent-tolerant lipase from Bacillus sp. strain 42 - Khusaini_2011_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_67_401 |
| Author(s) : Khusaini MS , Rahman RNZRA , Ali MSM , Leow ATC , Basri M , Salleh AB |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 67 :401 , 2011 |
| Abstract : |
| PubMedSearch : Khusaini_2011_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_67_401 |
| PubMedID: 21393852 |
| Gene_locus related to this paper: bacsp-lip |
| Title : Crystallization and preliminary X-ray crystallographic analysis of highly thermostable L2 lipase from the newly isolated Bacillus sp. L2 - Shariff_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_715 |
| Author(s) : Shariff FM , Rahman RNZRA , Ali MSM , Chor AL , Basri M , Salleh AB |
| Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 66 :715 , 2010 |
| Abstract : |
| PubMedSearch : Shariff_2010_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_66_715 |
| PubMedID: 20516608 |
| Gene_locus related to this paper: bacsp-lip |
| Title : Activation of bacterial thermoalkalophilic lipases is spurred by dramatic structural rearrangements - Carrasco-Lopez_2009_J.Biol.Chem_284_4365 |
| Author(s) : Carrasco-Lopez C , Godoy C , de Las Rivas B , Fernandez-Lorente G , Palomo JM , Guisan JM , Fernandez-Lafuente R , Martinez-Ripoll M , Hermoso JA |
| Ref : Journal of Biological Chemistry , 284 :4365 , 2009 |
| Abstract : |
| PubMedSearch : Carrasco-Lopez_2009_J.Biol.Chem_284_4365 |
| PubMedID: 19056729 |
| Gene_locus related to this paper: bactc-lipas |
| Title : Novel cation-pi interaction revealed by crystal structure of thermoalkalophilic lipase - |
| Author(s) : Matsumura H , Yamamoto T , Leow ATC , Mori T , Salleh AB , Basri M , Inoue T , Kai Y , Rahman RNZRA |
| Ref : Proteins , 70 :592 , 2008 |
| PubMedID: 17932933 |
| Gene_locus related to this paper: bacsp-lip |
| Title : Novel zinc-binding center and a temperature switch in the Bacillus stearothermophilus L1 lipase - Jeong_2002_J.Biol.Chem_277_17041 |
| Author(s) : Jeong ST , Kim HK , Kim SJ , Chi SW , Pan JG , Oh TK , Ryu SE |
| Ref : Journal of Biological Chemistry , 277 :17041 , 2002 |
| Abstract : |
| PubMedSearch : Jeong_2002_J.Biol.Chem_277_17041 |
| PubMedID: 11859083 |
| Gene_locus related to this paper: geost-lipas |
| Title : Crystal Structure of a Thermostable Lipase from Bacillus stearothermophilus P1 - Tyndall_2002_J.Mol.Biol_323_859 |
| Author(s) : Tyndall JD , Sinchaikul S , Fothergill-Gilmore LA , Taylor P , Walkinshaw MD |
| Ref : Journal of Molecular Biology , 323 :859 , 2002 |
| Abstract : |
| PubMedSearch : Tyndall_2002_J.Mol.Biol_323_859 |
| PubMedID: 12417199 |
| Gene_locus related to this paper: geost-lipas |
| Title : Lipases for biotechnology - Jaeger_2002_Curr.Opin.Biotechnol_13_390 |
| Author(s) : Jaeger KE , Eggert T |
| Ref : Curr Opin Biotechnol , 13 :390 , 2002 |
| Abstract : |
| PubMedSearch : Jaeger_2002_Curr.Opin.Biotechnol_13_390 |
| PubMedID: 12323363 |
| Title : Bacterial lipolytic enzymes: classification and properties - Arpigny_1999_Biochem.J_343_177 |
| Author(s) : Arpigny JL , Jaeger KE |
| Ref : Biochemical Journal , 343 :177 , 1999 |
| Abstract : |
| PubMedSearch : Arpigny_1999_Biochem.J_343_177 |
| PubMedID: 10493927 |