Bradley_2020_Nat.Chem.Biol_16_240

Reference

Title : Biased M1-muscarinic-receptor-mutant mice inform the design of next-generation drugs - Bradley_2020_Nat.Chem.Biol_16_240
Author(s) : Bradley SJ , Molloy C , Valuskova P , Dwomoh L , Scarpa M , Rossi M , Finlayson L , Svensson KA , Chernet E , Barth VN , Gherbi K , Sykes DA , Wilson CA , Mistry R , Sexton PM , Christopoulos A , Mogg AJ , Rosethorne EM , Sakata S , John Challiss RA , Broad LM , Tobin AB
Ref : Nat Chemical Biology , 16 :240 , 2020
Abstract :

Cholinesterase inhibitors, the current frontline symptomatic treatment for Alzheimer's disease (AD), are associated with low efficacy and adverse effects. M1 muscarinic acetylcholine receptors (M1 mAChRs) represent a potential alternate therapeutic target; however, drug discovery programs focused on this G protein-coupled receptor (GPCR) have failed, largely due to cholinergic adverse responses. Employing novel chemogenetic and phosphorylation-deficient, G protein-biased, mouse models, paired with a toolbox of probe molecules, we establish previously unappreciated pharmacologically targetable M1 mAChR neurological processes, including anxiety-like behaviors and hyper-locomotion. By mapping the upstream signaling pathways regulating these responses, we determine the importance of receptor phosphorylation-dependent signaling in driving clinically relevant outcomes and in controlling adverse effects including 'epileptic-like' seizures. We conclude that M1 mAChR ligands that promote receptor phosphorylation-dependent signaling would protect against cholinergic adverse effects in addition to driving beneficial responses such as learning and memory and anxiolytic behavior relevant for the treatment of AD.

PubMedSearch : Bradley_2020_Nat.Chem.Biol_16_240
PubMedID: 32080630

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Bradley SJ, Molloy C, Valuskova P, Dwomoh L, Scarpa M, Rossi M, Finlayson L, Svensson KA, Chernet E, Barth VN, Gherbi K, Sykes DA, Wilson CA, Mistry R, Sexton PM, Christopoulos A, Mogg AJ, Rosethorne EM, Sakata S, John Challiss RA, Broad LM, Tobin AB (2020)
Biased M1-muscarinic-receptor-mutant mice inform the design of next-generation drugs
Nat Chemical Biology 16 :240

Bradley SJ, Molloy C, Valuskova P, Dwomoh L, Scarpa M, Rossi M, Finlayson L, Svensson KA, Chernet E, Barth VN, Gherbi K, Sykes DA, Wilson CA, Mistry R, Sexton PM, Christopoulos A, Mogg AJ, Rosethorne EM, Sakata S, John Challiss RA, Broad LM, Tobin AB (2020)
Nat Chemical Biology 16 :240