Buchholz_2022_Proteins_90_1443

Reference

Title : Plastics degradation by hydrolytic enzymes: The plastics-active enzymes database-PAZy - Buchholz_2022_Proteins_90_1443
Author(s) : Buchholz PCF , Feuerriegel G , Zhang H , Perez-Garcia P , Nover LL , Chow J , Streit WR , Pleiss J
Ref : Proteins , 90 :1443 , 2022
Abstract :

Petroleum-based plastics are durable and accumulate in all ecological niches. Knowledge on enzymatic degradation is sparse. Today, less than 50 verified plastics-active enzymes are known. First examples of enzymes acting on the polymers polyethylene terephthalate (PET) and polyurethane (PUR) have been reported together with a detailed biochemical and structural description. Furthermore, very few polyamide (PA) oligomer active enzymes are known. In this article, the current known enzymes acting on the synthetic polymers PET and PUR are briefly summarized, their published activity data were collected and integrated into a comprehensive open access database. The Plastics-Active Enzymes Database (PAZy) represents an inventory of known and experimentally verified enzymes that act on synthetic fossil fuel-based polymers. Almost 3000 homologs of PET-active enzymes were identified by profile hidden Markov models. Over 2000 homologs of PUR-active enzymes were identified by BLAST. Based on multiple sequence alignments, conservation analysis identified the most conserved amino acids, and sequence motifs for PET- and PUR-active enzymes were derived.

PubMedSearch : Buchholz_2022_Proteins_90_1443
PubMedID: 35175626

Citations formats

Buchholz PCF, Feuerriegel G, Zhang H, Perez-Garcia P, Nover LL, Chow J, Streit WR, Pleiss J (2022)
Plastics degradation by hydrolytic enzymes: The plastics-active enzymes database-PAZy
Proteins 90 :1443

Buchholz PCF, Feuerriegel G, Zhang H, Perez-Garcia P, Nover LL, Chow J, Streit WR, Pleiss J (2022)
Proteins 90 :1443