Choulet_2006_J.Bacteriol_188_6599

Reference

Title : Intraspecific variability of the terminal inverted repeats of the linear chromosome of Streptomyces ambofaciens - Choulet_2006_J.Bacteriol_188_6599
Author(s) : Choulet F , Gallois A , Aigle B , Mangenot S , Gerbaud C , Truong C , Francou FX , Borges F , Fourrier C , Guerineau M , Decaris B , Barbe V , Pernodet JL , Leblond P
Ref : Journal of Bacteriology , 188 :6599 , 2006
Abstract :

The sequences of the terminal inverted repeats (TIRs) ending the linear chromosomal DNA of two Streptomyces ambofaciens strains, ATCC23877 and DSM40697 (198 kb and 213 kb, respectively), were determined from two sets of recombinant cosmids. Among the 215 coding DNA sequences (CDSs) predicted in the TIRs of strain DSM40697, 65 are absent in the TIRs of strain ATCC23877. Reciprocally, 45 of the 194 predicted CDSs are specific to the ATCC23877 strain. The strain-specific CDSs are located mainly at the terminal end of the TIRs. Indeed, although TIRs appear almost identical over 150 kb (99% nucleotide identity), large regions of DNA of 60 kb (DSM40697) and 48 kb (ATCC23877), mostly spanning the ends of the chromosome, are strain specific. These regions are rich in plasmid-associated genes, including genes encoding putative conjugal transfer functions. The strain-specific regions also share a G+C content (68%) lower than that of the rest of the genome (from 71% to 73%), a percentage that is more typical of Streptomyces plasmids and mobile elements. These data suggest that exchanges of replicon extremities have occurred, thereby contributing to the terminal variability observed at the intraspecific level. In addition, the terminal regions include many mobile genetic element-related genes, pseudogenes, and genes related to adaptation. The results give insight into the mechanisms of evolution of the TIRs: integration of new information and/or loss of DNA fragments and subsequent homogenization of the two chromosomal extremities.

PubMedSearch : Choulet_2006_J.Bacteriol_188_6599
PubMedID: 16952952
Gene_locus related to this paper: stram-q0jwm8 , stram-q1rql3 , stram-q1rqp7 , stram-q1rqu8 , stram-q1rqw8 , stram-q1rr14 , stram-q1rr40 , stram-q1rr62 , stram-q1rrc9 , stram-q1rrf2 , stram-Q9KWX3

Related information

Gene_locus stram-q0jwm8    stram-q1rql3    stram-q1rqp7    stram-q1rqu8    stram-q1rqw8    stram-q1rr14    stram-q1rr40    stram-q1rr62    stram-q1rrc9    stram-q1rrf2    stram-Q9KWX3

Citations formats

Choulet F, Gallois A, Aigle B, Mangenot S, Gerbaud C, Truong C, Francou FX, Borges F, Fourrier C, Guerineau M, Decaris B, Barbe V, Pernodet JL, Leblond P (2006)
Intraspecific variability of the terminal inverted repeats of the linear chromosome of Streptomyces ambofaciens
Journal of Bacteriology 188 :6599

Choulet F, Gallois A, Aigle B, Mangenot S, Gerbaud C, Truong C, Francou FX, Borges F, Fourrier C, Guerineau M, Decaris B, Barbe V, Pernodet JL, Leblond P (2006)
Journal of Bacteriology 188 :6599