Del Giudice_2016_Biotechnol.Bioeng_113_724

Reference

Title : An efficient thermostable organophosphate hydrolase and its application in pesticide decontamination - Del Giudice_2016_Biotechnol.Bioeng_113_724
Author(s) : Del Giudice I , Coppolecchia R , Merone L , Porzio E , Carusone TM , Mandrich L , Worek F , Manco G
Ref : Biotechnol Bioeng , 113 :724 , 2016
Abstract :

In vitro evolution of enzymes represents a powerful device to evolve new or to improve weak enzymatic functions. In the present work a semi-rational engineering approach has been used to design an efficient and thermostable organophosphate hydrolase, starting from a lactonase scaffold (SsoPox from Sulfolobus solfataricus). In particular, by in vitro evolution of the SsoPox ancillary promiscuous activity, the triple mutant C258L/I261F/W263A has been obtained which, retaining its inherent stability, showed an enhancement of its hydrolytic activity on paraoxon up to 300-fold, achieving absolute values of catalytic efficiency up to 10(5) M(-1) s(-1) . The kinetics and structural determinants of this enhanced activity were thoroughly investigated and, in order to evaluate its potential biotechnological applications, the mutant was tested in formulations of different solvents (methanol or ethanol) or detergents (SDS or a commercial soap) for the cleaning of pesticide-contaminated surfaces. Biotechnol. Bioeng. 2016;113: 724-734. (c) 2015 Wiley Periodicals, Inc.

PubMedSearch : Del Giudice_2016_Biotechnol.Bioeng_113_724
PubMedID: 26416557

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Citations formats

Del Giudice I, Coppolecchia R, Merone L, Porzio E, Carusone TM, Mandrich L, Worek F, Manco G (2016)
An efficient thermostable organophosphate hydrolase and its application in pesticide decontamination
Biotechnol Bioeng 113 :724

Del Giudice I, Coppolecchia R, Merone L, Porzio E, Carusone TM, Mandrich L, Worek F, Manco G (2016)
Biotechnol Bioeng 113 :724