Delorme_2012_PLoS.One_7_e46493

Reference

Title : MmPPOX inhibits Mycobacterium tuberculosis lipolytic enzymes belonging to the hormone-sensitive lipase family and alters mycobacterial growth - Delorme_2012_PLoS.One_7_e46493
Author(s) : Delorme V , Diomande SV , Dedieu L , Cavalier JF , Carriere F , Kremer L , Leclaire J , Fotiadu F , Canaan S
Ref : PLoS ONE , 7 :e46493 , 2012
Abstract :

Lipid metabolism plays an important role during the lifetime of Mycobacterium tuberculosis, the causative agent of tuberculosis. Although M. tuberculosis possesses numerous lipolytic enzymes, very few have been characterized yet at a biochemical/pharmacological level. This study was devoted to the M. tuberculosis lipolytic enzymes belonging to the Hormone-Sensitive Lipase (HSL) family, which encompasses twelve serine hydrolases closely related to the human HSL. Among them, nine were expressed, purified and biochemically characterized using a broad range of substrates. In vitro enzymatic inhibition studies using the recombinant HSL proteins, combined with mass spectrometry analyses, revealed the potent inhibitory activity of an oxadiazolone compound, named MmPPOX. In addition, we provide evidence that MmPPOX alters mycobacterial growth. Overall, these findings suggest that the M. tuberculosis HSL family displays important metabolic functions, thus opening the way to further investigations linking the involvement of these enzymes in mycobacterial growth.

PubMedSearch : Delorme_2012_PLoS.One_7_e46493
PubMedID: 23029536
Gene_locus related to this paper: myctu-Rv2970c

Related information

Inhibitor MmPPOX
Substrate MmPPOX    delta-Valerolactone
Gene_locus MmPPOX    delta-Valerolactone    myctu-Rv2970c

Citations formats

Delorme V, Diomande SV, Dedieu L, Cavalier JF, Carriere F, Kremer L, Leclaire J, Fotiadu F, Canaan S (2012)
MmPPOX inhibits Mycobacterium tuberculosis lipolytic enzymes belonging to the hormone-sensitive lipase family and alters mycobacterial growth
PLoS ONE 7 :e46493

Delorme V, Diomande SV, Dedieu L, Cavalier JF, Carriere F, Kremer L, Leclaire J, Fotiadu F, Canaan S (2012)
PLoS ONE 7 :e46493