Diaz-Alejo_1998_Chem.Biol.Interact_108_187

Reference

Title : A stereospecific phosphotriesterase in hen liver and brain - Diaz-Alejo_1998_Chem.Biol.Interact_108_187
Author(s) : Diaz-Alejo N , Monroy A , Vilanova E , Vicedo JL , Sogorb MA
Ref : Chemico-Biological Interactions , 108 :187 , 1998
Abstract :

O-Hexyl, O-2,5-dichlorophenyl phosphoramidate (HDCP) is a chiral compound that induces delayed neuropathy in hens. This compound is hydrolyzed by a phosphotriesterase known as HDCPase in hen and rat plasma, liver and brain. We studied the stereospecificity of HDCPase in hen tissues and in human and rabbit plasma employing a chromatographic method for analysis and quantification of HDCP stereoisomers. Hen and human plasma HDCPases were not stereospecific. However, rabbit plasma showed a remarkable stereospecificity to S-(-)-HDCP. High levels of stereospecific HDCPase were found in the particulate fraction of hen liver, where S-(-)-HDCP is hydrolyzed faster than R-(+)-HDCP. However, in hen brain the stereospecificity was found in the soluble fraction, where R-(+)-HDCP is hydrolyzed faster than S-(-)-HDCP. It is concluded that liver particulate fraction must be the main tissue responsible for the HDCP stereospecific biotransformation in hens. In an oral administration, the steroisomer R-(+)-HDCP would survive after passing through the liver and would interact with acetylcholinesterase and neuropathy target esterase in the nervous system.

PubMedSearch : Diaz-Alejo_1998_Chem.Biol.Interact_108_187
PubMedID: 9528689

Related information

Inhibitor Hexyl-dcp

Citations formats

Diaz-Alejo N, Monroy A, Vilanova E, Vicedo JL, Sogorb MA (1998)
A stereospecific phosphotriesterase in hen liver and brain
Chemico-Biological Interactions 108 :187

Diaz-Alejo N, Monroy A, Vilanova E, Vicedo JL, Sogorb MA (1998)
Chemico-Biological Interactions 108 :187