Du_2013_Eukaryot.Cell_12_1517

Reference

Title : Dictyostelium lipid droplets host novel proteins - Du_2013_Eukaryot.Cell_12_1517
Author(s) : Du X , Barisch C , Paschke P , Herrfurth C , Bertinetti O , Pawolleck N , Otto H , Ruhling H , Feussner I , Herberg FW , Maniak M
Ref : Eukaryot Cell , 12 :1517 , 2013
Abstract :

Across all kingdoms of life, cells store energy in a specialized organelle, the lipid droplet. In general, it consists of a hydrophobic core of triglycerides and steryl esters surrounded by only one leaflet derived from the endoplasmic reticulum membrane to which a specific set of proteins is bound. We have chosen the unicellular organism Dictyostelium discoideum to establish kinetics of lipid droplet formation and degradation and to further identify the lipid constituents and proteins of lipid droplets. Here, we show that the lipid composition is similar to what is found in mammalian lipid droplets. In addition, phospholipids preferentially consist of mainly saturated fatty acids, whereas neutral lipids are enriched in unsaturated fatty acids. Among the novel protein components are LdpA, a protein specific to Dictyostelium, and Net4, which has strong homologies to mammalian DUF829/Tmem53/NET4 that was previously only known as a constituent of the mammalian nuclear envelope. The proteins analyzed so far appear to move from the endoplasmic reticulum to the lipid droplets, supporting the concept that lipid droplets are formed on this membrane.

PubMedSearch : Du_2013_Eukaryot.Cell_12_1517
PubMedID: 24036346
Gene_locus related to this paper: dicdi-q54yr8 , human-TMEM53

Related information

Gene_locus dicdi-q54yr8    human-TMEM53
Family dicdi-q54yr8    human-TMEM53    Duf_829

Citations formats

Du X, Barisch C, Paschke P, Herrfurth C, Bertinetti O, Pawolleck N, Otto H, Ruhling H, Feussner I, Herberg FW, Maniak M (2013)
Dictyostelium lipid droplets host novel proteins
Eukaryot Cell 12 :1517

Du X, Barisch C, Paschke P, Herrfurth C, Bertinetti O, Pawolleck N, Otto H, Ruhling H, Feussner I, Herberg FW, Maniak M (2013)
Eukaryot Cell 12 :1517