Duf_829
Comment
Protein of unknown function DUF829. Contains exclusively eukaryote proteins including transmembrane protein 53. Said to be integral membrane proteins (!?) Dictyostelium discoideum Net4 (dicdi-q54yr8), which has strong homologies to mammalian DUF829/Tmem53/NET4 is found on lipid droplets (Du et al.). Seems to have a conserved catalytic triad Ser-113, Asp-220, and His-252 in TMEM53_HUMAN. Korfali et al. (2011) identified NET4 as a gene that influenced cell cycle regulation. The cell cycle effects of NET4 were dependent on p53 and RB. Knockdown of NET4 caused premature senescence due to permanent arrest of the cell cycle in MRC5 human fibroblasts, whereas it only caused a cell cycle delay at G1 phase in U2OS cells. 
Guo et al. (2021) analyzed the molecular pathophysiology of Tmem53 -/- mice using primary cells from the mutant mice and TMEM53 knockout cell lines and found that TMEM53 inhibits BMP signaling in osteoblast lineage cells by blocking cytoplasm-nucleus translocation of BMP2-activated Smad proteins. Deficiency of TMEM53 causes a previously unknown sclerosing bone disorder by dysregulation of BMP-SMAD signaling (Guo et al.2021).TMEM53 is a novel SADS-CoV restriction factor that targets viral RNA-dependent RNA polymerase Yao et al. (2023)
Database
Interpro : IPR008547 DUF829 Protein of unknown function DUF829, TMEM53 DUF829_TMEM53
PIRSF : No PIRSF
Pdoc : No Pdoc
Pfam : PF05705 DUF829
Prints : No Print
EC Number : No EC Number
Sequences
| Peptide in | Fasta |
| Nucleotide in | Fasta |
| Alignment with Multalin | Text only |
| Seed alignment with MAFFT | No colour / coloured with Mview |
| Alignment with MAFFT | No colour / coloured with Mview |
| Dendrogram | The dnd file |
| Gene_locus | Name | Species |
|---|---|---|
| aedae-q16rz9 | Aedes aegypti (Yellowfever mosquito) Putative uncharacterized protein | Aedes aegypti |
| aedae-q16xw5 | Aedes aegypti (Yellowfever mosquito) Putative uncharacterized protein | Aedes aegypti |
| aedae-q16xw6 | Aedes aegypti (Yellowfever mosquito) Putative uncharacterized protein | Aedes aegypti |
| ajecn-a6qxh2 | Ajellomyces capsulata (Histoplasma capsulatum) (strain NAm1 \/ WU24) (Darling's disease fungus Predicted protein | Ajellomyces capsulata |
| ajecn-a6rab7 | Ajellomyces capsulata (Histoplasma capsulatum) (Darling's disease fungus Predicted protein | Ajellomyces capsulata |
| anoga-q5tpv0 | Anopheles gambiae (African malaria mosquito) AGAP011464-PA | Anopheles gambiae |
| anoga-q7pm39 | Anopheles gambiae (African malaria mosquito) AGAP009586-PA | Anopheles gambiae |
| arath-q8s8g6 | Arabidopsis thaliana (Mouse-ear cress) Putative uncharacterized protein Expressed protein (At2g18245) | Arabidopsis thaliana |
| arath-q8s8h8 | Arabidopsis thaliana (Mouse-ear cress) Putative uncharacterized protein At2g15695 | Arabidopsis thaliana |
| arath-q9ffg7 | Arabidopsis thaliana (Mouse-ear cress) Putative uncharacterized protein At5g44250\; MLN1.18) | Arabidopsis thaliana |
| arath-q9lhe8 | Arabidopsis thaliana (Mouse-ear cress) Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress) Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone: MZE19 (At3g19970\/MZE19_2) | Arabidopsis thaliana |
| aspcl-a1c5a7 | Aspergillus clavatus Putative uncharacterized protein | Aspergillus clavatus |
| aspcl-a1chj6 | Aspergillus clavatus Putative uncharacterized protein | Aspergillus clavatus |
| aspcl-a1cpk9 | Aspergillus clavatus DNA repair protein (Rad57), putative | Aspergillus clavatus |
| aspcl-a1cs39 | Aspergillus clavatus Putative uncharacterized protein | Aspergillus clavatus |
| aspfu-q4wa39 | Aspergillus fumigatus (Sartorya fumigata) DUF829 domain protein (PaxU), putative | Aspergillus fumigatus |
| aspfu-q4wkh6 | Aspergillus fumigatus (Sartorya fumigata) Indole-diterpene biosynthesis protein PaxU, putative | Aspergillus fumigatus |
| aspng-a2q8w0 | Aspergillus niger Contig An01c0200, complete genome | Aspergillus niger |
| aspng-a2r0p8 | Aspergillus niger Similarity to hypothetical protein PaxU -Penicillium paxilli | Aspergillus niger |
| aspng-a2r225 | Aspergillus niger Contig An13c0090, complete genome | Aspergillus niger |
| aspor-q2u3k5 | Aspergillus oryzae Predicted protein | Aspergillus oryzae |
| aspor-q2urf3 | Aspergillus oryzae Predicted protein | Aspergillus oryzae |
| asptn-q0cn88 | Aspergillus terreus (strain NIH 2624) Putative uncharacterized protein | Aspergillus terreus |
| asptn-q0d199 | Aspergillus terreus (strain NIH 2624) Predicted protein | Aspergillus terreus |
| botfb-a6s209 | Botryotinia fuckeliana (strain B05.10) (Noble rot fungus) (Botrytis cinerea) Putative uncharacterized protein | Botryotinia fuckeliana |
| bovin-tmm53 | Bos taurus (Bovine) Transmembrane protein 53 | Bos taurus |
| caeel-q17846 | Caenorhabditis elegans Putative uncharacterized protein C09B8.4 | Caenorhabditis elegans |
| caeel-q22750 | Caenorhabditis elegans Protein T24H10.4, partially confirmed by transcript evidence | Caenorhabditis elegans |
| caeel-q9tzh8 | Caenorhabditis elegans Putative uncharacterized protein T10B11.6 | Caenorhabditis elegans |
| caeel-q9tzh9 | Caenorhabditis elegans Putative uncharacterized protein T10B11.5 | Caenorhabditis elegans |
| chagb-q2gmj1 | Chaetomium globosum (Soil fungus) Putative uncharacterized protein | Chaetomium globosum |
| chagb-q2gmx4 | Chaetomium globosum (Soil fungus) Putative uncharacterized protein | Chaetomium globosum |
| chagb-q2gpy6 | Chaetomium globosum (Soil fungus) Putative uncharacterized protein | Chaetomium globosum |
| cocim-j3k8a1 | Coccidioides immitis, Coccidioides posadasii (Valley fever fungus), Putative uncharacterized protein | Coccidioides immitis |
| danre-a3kpm9 | Danio rerio (Zebrafish) (Brachydanio rerio) Novel protein | Danio rerio |
| danre-tmm53 | Danio rerio (Zebrafish) (Brachydanio rerio) Transmembrane protein 53 | Danio rerio |
| dicdi-q54gw5 | Dictyostelium discoideum (Slime mold) Putative uncharacterized protein | Dictyostelium discoideum |
| dicdi-q54n85 | Dictyostelium discoideum (Slime mold) Putative transmembrane protein | Dictyostelium discoideum |
| dicdi-q54xu1 | Dictyostelium discoideum (Slime mold) Putative uncharacterized protein | Dictyostelium discoideum |
| dicdi-q54xu2 | Dictyostelium discoideum (Slime mold) Putative uncharacterized protein | Dictyostelium discoideum |
| dicdi-q54yr8 | Dictyostelium discoideum (Slime mold) Putative transmembrane protein | Dictyostelium discoideum |
| dicdi-q557k5 | Dictyostelium discoideum (Slime mold) Putative uncharacterized protein | Dictyostelium discoideum |
| dicdi-q86iz7 | Dictyostelium discoideum (Slime mold) Putative uncharacterized protein | Dictyostelium discoideum |
| drome-a1z6g9 | Drosophila melanogaster (Fruit fly) CG8245-RA | Drosophila melanogaster |
| drome-q7k274 | Drosophila melanogaster (Fruit fly) LD15586p (CG3082-PA, isoform A) (CG3082-PC, isoform C) CG3082-PD, isoform D (RH35959p) | Drosophila melanogaster |
| drops-q291y3 | Drosophila pseudoobscura (Fruit fly) GA15913-PA | Drosophila pseudoobscura |
| drops-q29k25 | Drosophila pseudoobscura (Fruit fly) GA20925-PA | Drosophila pseudoobscura |
| droya-q71d76 | Drosophila yakuba (Fruit fly) 1(2)k09913 Drosophila erecta L(2)k09913-PA (Lethal (2) k09913) | Drosophila yakuba |
| emeni-q5b1v2 | Emericella nidulans (Aspergillus nidulans) Putative uncharacterized protein | Emericella nidulans |
| emeni-q5bar0 | Emericella nidulans (Aspergillus nidulans) Putative uncharacterized protein | Emericella nidulans |
| emeni-q5beh9 | Emericella nidulans (Aspergillus nidulans) Putative uncharacterized protein | Emericella nidulans |
| emeni-q5bgk7 | Emericella nidulans (Aspergillus nidulans) Putative uncharacterized protein | Emericella nidulans |
| human-TMEM53 | Homo sapiens (Human) Transmembrane protein 53, FLJ22353, NET4 | Homo sapiens |
| maggr-a4r8c0 | Magnaporthe grisea (Rice blast fungus) (Pyricularia grisea) Putative uncharacterized protein | Magnaporthe grisea |
| mouse-tmm53 | Mus musculus (Mouse) Transmembrane protein 53 | Mus musculus |
| nemve-a7shf2 | Nematostella vectensis (Starlet sea anemone) Predicted protein | Nematostella vectensis |
| neofi-a1d512 | Neosartorya fischeri (Aspergillus fischerianus(strain ATCC 1020 \/ DSM 3700 \/ NRRL 181)) Putative uncharacterized protein | Neosartorya fischeri |
| neofi-a1da18 | Neosartorya fischeri (Aspergillus fischerianus (strain ATCC 1020 \/ DSM 3700 \/ NRRL 181)) Putative uncharacterized protein | Neosartorya fischeri |
| neucr-q7s2c5 | Neurospora crassa Predicted protein | Neurospora crassa |
| neucr-q7s2p4 | Neurospora crassa Predicted protein | Neurospora crassa |
| neucr-q872l1 | Neurospora crassa Putative uncharacterized protein 64C2.020 | Neurospora crassa |
| orysi-a2xc83 | Oryza sativa subsp. indica (Rice) Oryza sativa subsp. japonica (Rice) Putative uncharacterized protein | Oryza sativa |
| orysi-a2yh83 | Oryza sativa subsp. indica (Rice) Putative uncharacterized protein | Oryza sativa subsp. indica |
| orysj-a3b9l8 | Oryza sativa subsp. japonica (Rice) Oryza sativa subsp. indica Putative uncharacterized protein | Oryza sativa |
| orysj-q0d9a4 | Oryza sativa subsp. japonica (Rice) Os06g0730300 protein | Oryza sativa subsp. japonica |
| orysj-q5z419 | Oryza sativa subsp. japonica (Rice) Integral membrane protein-like (Putative uncharacterized protein) | Oryza sativa subsp. japonica |
| orysj-q6z6i1 | Oryza sativa subsp. japonica indica (Rice) Putative uncharacterized protein P0030G11.19 (Os02g0133900 protein) (Putative uncharacterized protein P0585B01.4) | Oryza sativa subsp. japonica |
| ostlu-a4s4v4 | Ostreococcus lucimarinus (strain CCE9901) Predicted protein | Ostreococcus lucimarinus |
| ostta-q00ys6 | Ostreococcus tauri [S] KOG2521 Uncharacterized conserved protein | Ostreococcus tauri |
| penpx-q9c445 | Penicillium paxilli PaxU | Penicillium paxilli |
| phano-q0u2y5 | Phaeosphaeria nodorum (Septoria nodorum) Putative uncharacterized protein | Phaeosphaeria nodorum |
| phano-q0u3i1 | Phaeosphaeria nodorum (Septoria nodorum) Putative uncharacterized protein | Phaeosphaeria nodorum |
| scls1-a7eam7 | Sclerotinia sclerotiorum (Whetzelinia sclerotiorum) (strain ATCC 18683 \/ 1980 \/ Ss-1) Putative uncharacterized protein | Sclerotinia sclerotiorum |
| scls1-a7f797 | Sclerotinia sclerotiorum (Whetzelinia sclerotiorum)(strain ATCC 18683 \/ 1980 \/ Ss-1) (White mold) Putative uncharacterized protein | Sclerotinia sclerotiorum |
| tetng-q4rjp3 | Tetraodon nigroviridis (Spotted green pufferfish) (Chelonodon nigroviridis) Chromosome 13 SCAF15035, whole genome shotgun sequence | Tetraodon nigroviridis |
| tetng-q4sbu0 | Tetraodon nigroviridis (Spotted green pufferfish) (Chelonodon nigroviridis) Chromosome 19 SCAF14664, whole genome shotgun sequence | Tetraodon nigroviridis |
| tetng-q4snp0 | Tetraodon nigroviridis (Spotted green pufferfish) (Chelonodon nigroviridis) Chromosome 15 SCAF14542, whole genome shotgun sequence | Tetraodon nigroviridis |
| triva-a2e7p9 | Trichomonas vaginalis G3 Putative uncharacterized protein | Trichomonas vaginalis G3 |
| triva-a2fhp7 | Trichomonas vaginalis G3 Putative uncharacterized protein | Trichomonas vaginalis G3 |
| triva-a2fz57 | Trichomonas vaginalis G3 Putative uncharacterized protein | Trichomonas vaginalis G3 |
| ustma-q4p5d2 | Ustilago maydis (Smut fungus) Putative uncharacterized protein | Ustilago maydis |
| vitvi-a7ntu2 | Vitis vinifera (Grape) Chromosome chr18 scaffold_1, whole genome shotgun sequence | Vitis vinifera |
| vitvi-a7pus9 | Vitis vinifera (Grape) Chromosome chr4 scaffold_32, whole genome shotgun sequence | Vitis vinifera |
| vitvi-a7qpz3 | Vitis vinifera (Grape) Chromosome undetermined scaffold_139, whole genome shotgun sequence | Vitis vinifera |
| vitvi-d7tpk8 | Vitis vinifera (Grape) Chromosome chr3 scaffold_8, whole genome shotgun sequence | Vitis vinifera |
| xenla-tm53a | Xenopus laevis (African clawed frog) Transmembrane protein 53-A | Xenopus laevis |
| xenla-tm53b | Xenopus laevis (African clawed frog) Transmembrane protein 53-B | Xenopus laevis |
| Gene_locus_frgt | Name | Species |
|---|---|---|
| 9bras-d6pq43 | Capsella grandiflora AT3G19970-like protein | Capsella grandiflora |
| anoga-a7uuu5 | Anopheles gambiae (African malaria mosquito) AGAP011465-PA | Anopheles gambiae |
| anoga-q7pn09 | Anopheles gambiae (African malaria mosquito) AGAP011466-PA | Anopheles gambiae |
| artsf-a3f587 | Artemia sanfranciscana (Brine shrimp) (Artemia franciscana) Putative uncharacterized protein | Artemia sanfranciscana |
| nemve-a7rgu8 | Nematostella vectensis (Starlet sea anemone) Predicted protein | Nematostella vectensis |
| neofi-a1d2h1 | Neosartorya fischeri(Aspergillus fischerianus (strain ATCC 1020 \/ DSM 3700 \/ NRRL 181 DNA repair protein (Rad57))), putative | Neosartorya fischeri |
| ostlu-a4rr84 | Ostreococcus lucimarinus (strain CCE9901) Predicted protein | Ostreococcus lucimarinus |
| phano-q0ty09 | Phaeosphaeria nodorum (Septoria nodorum) Putative uncharacterized protein | Phaeosphaeria nodorum |
References (4)
| Title : Identification of TMEM53 as a novel SADS-CoV restriction factor that targets viral RNA-dependent RNA polymerase - Yao_2023_Emerg.Microbes.Infect_12_2249120 |
| Author(s) : Yao YL , Luo Y , Wang Q , Geng R , Chen Y , Liu MQ , Li B , Chen J , Wu CG , Jia JK , Luo JY , He YT , Jiang TT , Zhu Y , Hu B , Zhou P , Shi ZL |
| Ref : Emerg Microbes Infect , 12 :2249120 , 2023 |
| Abstract : |
| PubMedSearch : Yao_2023_Emerg.Microbes.Infect_12_2249120 |
| PubMedID: 37584551 |
| Gene_locus related to this paper: human-TMEM53 |
| Title : Deficiency of TMEM53 causes a previously unknown sclerosing bone disorder by dysregulation of BMP-SMAD signaling - Guo_2021_Nat.Commun_12_2046 |
| Author(s) : Guo L , Iida A , Bhavani GS , Gowrishankar K , Wang Z , Xue JY , Wang J , Miyake N , Matsumoto N , Hasegawa T , Iizuka Y , Matsuda M , Nakashima T , Takechi M , Iseki S , Yambe S , Nishimura G , Koseki H , Shukunami C , Girisha KM , Ikegawa S |
| Ref : Nat Commun , 12 :2046 , 2021 |
| Abstract : |
| PubMedSearch : Guo_2021_Nat.Commun_12_2046 |
| PubMedID: 33824347 |
| Gene_locus related to this paper: human-TMEM53 |
| Title : Dictyostelium lipid droplets host novel proteins - Du_2013_Eukaryot.Cell_12_1517 |
| Author(s) : Du X , Barisch C , Paschke P , Herrfurth C , Bertinetti O , Pawolleck N , Otto H , Ruhling H , Feussner I , Herberg FW , Maniak M |
| Ref : Eukaryot Cell , 12 :1517 , 2013 |
| Abstract : |
| PubMedSearch : Du_2013_Eukaryot.Cell_12_1517 |
| PubMedID: 24036346 |
| Gene_locus related to this paper: dicdi-q54yr8 , human-TMEM53 |
| Title : A flow cytometry-based screen of nuclear envelope transmembrane proteins identifies NET4\/Tmem53 as involved in stress-dependent cell cycle withdrawal - Korfali_2011_PLoS.One_6_e18762 |
| Author(s) : Korfali N , Srsen V , Waterfall M , Batrakou DG , Pekovic V , Hutchison CJ , Schirmer EC |
| Ref : PLoS ONE , 6 :e18762 , 2011 |
| Abstract : |
| PubMedSearch : Korfali_2011_PLoS.One_6_e18762 |
| PubMedID: 21533191 |
| Gene_locus related to this paper: human-TMEM53 |