Family: Duf_829

Block: X

Parent Family: No family


Protein of unknown function DUF829. Contains exclusively eukaryote proteins including transmembrane protein 53. Said to be integral membrane proteins (!?) Dictyostelium discoideum Net4 (dicdi-q54yr8), which has strong homologies to mammalian DUF829/Tmem53/NET4 is found on lipid droplets (Du et al.). Seems to have a conserved catalytic triad Ser-113, Asp-220, and His-252 in TMEM53_HUMAN. Korfali et al. (2011) identified NET4 as a gene that influenced cell cycle regulation. The cell cycle effects of NET4 were dependent on p53 and RB. Knockdown of NET4 caused premature senescence due to permanent arrest of the cell cycle in MRC5 human fibroblasts, whereas it only caused a cell cycle delay at G1 phase in U2OS cells. Guo et al. (2021) analyzed the molecular pathophysiology of Tmem53 -/- mice using primary cells from the mutant mice and TMEM53 knockout cell lines and found that TMEM53 inhibits BMP signaling in osteoblast lineage cells by blocking cytoplasm-nucleus translocation of BMP2-activated Smad proteins. Deficiency of TMEM53 causes a previously unknown sclerosing bone disorder by dysregulation of BMP-SMAD signaling (Guo et al.2021)


Interpro : IPR008547 DUF829 Protein of unknown function DUF829, TMEM53 DUF829_TMEM53


Pdoc : No Pdoc

Pfam : PF05705 DUF829

Prints : No Print

EC Number : No EC Number


Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour
Alignment with MAFFT No colour
Dendrogram The dnd file

No structure scheme yet for this family

Genes Proteins in Duf_829 family (87)

Fragments of genes in Duf_829 family (8)

No fragment of genes

No substrate

No Inhibitor

References (3)

Title : Deficiency of TMEM53 causes a previously unknown sclerosing bone disorder by dysregulation of BMP-SMAD signaling - Guo_2021_Nat.Commun_12_2046
Author(s) : Guo L , Iida A , Bhavani GS , Gowrishankar K , Wang Z , Xue JY , Wang J , Miyake N , Matsumoto N , Hasegawa T , Iizuka Y , Matsuda M , Nakashima T , Takechi M , Iseki S , Yambe S , Nishimura G , Koseki H , Shukunami C , Girisha KM , Ikegawa S
Ref : Nat Commun , 12 :2046 , 2021
PubMedID: 33824347
Gene_locus related to this paper: human-TMEM53

Title : Dictyostelium lipid droplets host novel proteins - Du_2013_Eukaryot.Cell_12_1517
Author(s) : Du X , Barisch C , Paschke P , Herrfurth C , Bertinetti O , Pawolleck N , Otto H , Ruhling H , Feussner I , Herberg FW , Maniak M
Ref : Eukaryot Cell , 12 :1517 , 2013
PubMedID: 24036346
Gene_locus related to this paper: dicdi-q54yr8 , human-TMEM53

Title : A flow cytometry-based screen of nuclear envelope transmembrane proteins identifies NET4\/Tmem53 as involved in stress-dependent cell cycle withdrawal - Korfali_2011_PLoS.One_6_e18762
Author(s) : Korfali N , Srsen V , Waterfall M , Batrakou DG , Pekovic V , Hutchison CJ , Schirmer EC
Ref : PLoS ONE , 6 :e18762 , 2011
PubMedID: 21533191
Gene_locus related to this paper: human-TMEM53