| Title : Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure - Eggert_2001_FEBS.Lett_502_89 |
| Author(s) : Eggert T , van Pouderoyen G , Dijkstra BW , Jaeger KE |
| Ref : FEBS Letters , 502 :89 , 2001 |
|
Abstract :
Bacillus subtilis secretes the lipolytic enzymes LipA and LipB. We show here that they are differentially expressed depending on the composition of the growth medium: LipA is produced in rich and in minimal medium, whereas LipB is present only in rich medium. A comparison of biochemical characteristics revealed that LipB is thermostable at pH 11 but becomes thermolabile at pH 5. However, construction of a variant carrying the substitution A76G in the conserved lipase pentapeptide reversed these effects. The atomic coordinates from the LipA crystal structure were used to build a three-dimensional structural model of LipB, which revealed that 43 out of 45 residues different from LipA are surface-located allowing to rationalize the differences observed in the substrate preferences of the two enzymes. |
| PubMedSearch : Eggert_2001_FEBS.Lett_502_89 |
| PubMedID: 11583117 |
| Gene_locus related to this paper: bacsu-lip , bacsu-LIPB |
| Gene_locus | bacsu-lip bacsu-LIPB |
| Family | Lipase_2 |
Eggert T, van Pouderoyen G, Dijkstra BW, Jaeger KE (2001)
Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure
FEBS Letters
502 :89
Eggert T, van Pouderoyen G, Dijkstra BW, Jaeger KE (2001)
FEBS Letters
502 :89