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bacsu-lip

Bacillus subtilis lipase (lipA) (EC 3.1.1.3) BSLA (triacylglycerol lipase)

Comment

One of the smallest lipase. It lacks a lid covering the substrate cleft.the active Ser in AXSXG pentapeptide.Prefers substrates with C8 fatty acid chains It shows remarkable stability at low pH. Also called LIPA or ESTA as it is more an esterase than a lipase. Bacillus subtilis (subsp. spizizenii (ATCC 23059 \/ NRRL B-14472 \/ W23\; ATCC 6633)\; BSn5\; subsp. natto BEST195\; 168)

Relationship

Family : Lipase_2

Block : X

Position in NCBI Life Tree : Bacillus subtilis

(Below N is a link to NCBI taxonomic web page and E link to ESTHER at designed phylum.)

> cellular organisms N E > Bacteria N E > Terrabacteria group N E > Firmicutes N E > Bacilli N E > Bacillales N E > Bacillaceae N E > Bacillus N E > Bacillus subtilis group N E > Bacillus subtilis N E

Molecular evidence

No mutation

1I6W - The crystal structure of bacillus subtilis lipase: a minimal alpha\/beta hydrolase enzyme 1ISP - Crystal structure of Bacillus subtilis lipase at 1.3A resolution 1R4Z - Bacillus subtilis lipase A with covalently bound Rc-IPG-phosphonate-inhibitor 1R50 - Bacillus subtilis lipase A with covalently bound Sc-IPG-phosphonate-inhibitor 1T2N - Structure of a thermostable triple mutant of Bacillus subtilis lipase obtained through directed evolution N166Y\/A132D\/L114P 1T4M - Structure of a thermostable mutant of Bacillus subtilis lipase obtained through directed evolution N166Y\/A132D 2QXT - Crystal Structure Analysis of the Bacillus subtilis lipase crystallized at pH 4.5 2QXU - Crystal Structure Analysis of the Bacillus subtilis lipase crystallized at pH 5.0 3D2A - Structure of 1-17A4, a thermostable mutant of Bacillus subtilis lipase obtained through directed evolution 3D2B - Structure of 2D9, a thermostable mutant of Bacillus subtilis lipase obtained through directed evolution 3D2C - Structure of 4D3, a thermostable mutant of Bacillus subtilis lipase obtained through directed evolution 3QMM - Structure of 6B, a thermostable mutant of Bacillus subtilis lipase obtained through directed evolution 3QZU - Crystal structure of Bacillus subtilis Lipase A 7-fold mutant\; the outcome of directed evolution towards thermostability 5CRI - Wild-type lipase A from Bacillus subtilis resolved with 0 \% v\/v [BMIM][Cl] 5CT4 - Wild-type lipase A from Bacillus subtilis resolved with 0 \% v\/v [BMIM][Cl] 5CT5 - Wild-type lipase A from Bacillus subtilis resolved with 0 \% v\/v [BMIM][Cl] 5CT6 - Wild-type lipase 20 \% IL soaking 5CT8 - Mutant lipase with no IL 5CT9 - G158E\/K44E\/R57E\/Y49E Bacillus subtilis lipase A with 5\% [BMIM][Cl] 5CTA - G158E\/K44E\/R57E\/Y49E Bacillus subtilis lipase A with 10\% [BMIM][Cl] 5CUR - G158E\/K44E\/R57E\/Y49E Bacillus subtilis lipase A with 20\% [BMIM][Cl]

No kinetic

No disease

Database

Sequence

Peptide

MKFVKRRIIA LVTILMLSVT SLFALQPSAK AAEHNPVVMV HGIGGASFNF AGIKSYLVSQ GWSRDKLYAV DFWDKTGTNY NNGPVLSRFV QKVLDETGAK KVDIVAHSMG GANTLYYIKN LDGGNKVANV VTLGGANRLT TGKALPGTDP NQKILYTSIY SSADMIVMNY LSRLDGARNV QIHGVGHIGL LYSSQVNSLI KEGLNGGGQN TN

References (32)

Title : Hydrophobic Clusters Regulate Surface Hydration Dynamics of Bacillus subtilis Lipase A - Jaufer_2024_J.Phys.Chem.B__
Author(s) : Jaufer AM , Bouhadana A , Fanucci GE
Ref : J Phys Chem B , : , 2024
Abstract :
PubMedSearch : Jaufer_2024_J.Phys.Chem.B__
PubMedID: 38628066
Gene_locus related to this paper: bacsu-lip

Title : Corner Engineering: Tailoring Enzymes for Enhanced Resistance and Thermostability in Deep Eutectic Solvents - Wang_2023_Angew.Chem.Int.Ed.Engl__e202315125
Author(s) : Wang X , Sheng Y , Cui H , Qiao J , Song Y , Li X , Huang H
Ref : Angew Chem Int Ed Engl , :e202315125 , 2023
Abstract :
PubMedSearch : Wang_2023_Angew.Chem.Int.Ed.Engl__e202315125
PubMedID: 38010210
Gene_locus related to this paper: bacsu-lip , bacsu-pnbae

Title : Lipase A from Bacillus subtilis: Substrate Binding, Conformational Dynamics, and Signatures of a Lid - Behera_2023_J.Chem.Inf.Model__
Author(s) : Behera S , Balasubramanian S
Ref : J Chem Inf Model , : , 2023
Abstract :
PubMedSearch : Behera_2023_J.Chem.Inf.Model__
PubMedID: 37989487
Gene_locus related to this paper: bacsu-lip

Title : Polar substitutions on the surface of a lipase substantially improve tolerance in organic solvents - Davari_2022_ChemSusChem__
Author(s) : Davari MD , Cui H , Vedder M , Zhang L , Jaeger KE , Schwaneberg U
Ref : ChemSusChem , : , 2022
Abstract :
PubMedSearch : Davari_2022_ChemSusChem__
PubMedID: 35007408
Gene_locus related to this paper: bacsu-lip

Title : Using Molecular Simulation to Guide Protein Engineering for Biocatalysis in Organic Solvents - Cui_2022_Methods.Mol.Biol_2397_179
Author(s) : Cui H , Vedder M , Schwaneberg U , Davari MD
Ref : Methods Mol Biol , 2397 :179 , 2022
Abstract :
PubMedSearch : Cui_2022_Methods.Mol.Biol_2397_179
PubMedID: 34813065
Gene_locus related to this paper: bacsu-lip

Title : Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET - Magalhaes_2021_Int.J.Mol.Sci_22_11257
Author(s) : Magalhaes RP , Cunha JM , Sousa SF
Ref : Int J Mol Sci , 22 :11257 , 2021
Abstract :
PubMedSearch : Magalhaes_2021_Int.J.Mol.Sci_22_11257
PubMedID: 34681915
Gene_locus related to this paper: 9entr-EsEstB , 9bact-a0a2h5z9r5 , 9psed-peh , 9actn-h6wx58 , idesa-mheth , idesa-peth , thecd-d1a2h1 , humin-cut , 9acto-d4q9n1 , 9acto-f7ix06 , 9bact-g9by57 , bacsu-lip , bacsu-pnbae , canar-LipB , thela-m4tp71 , psemy-a4y035 , thefu-1831 , thefu-q6a0i4 , strsw-c9zcr8 , fusox-CUT , sacvd-c7mve8

Title : Effect of Organic Solvents on the Structure and Activity of a Minimal Lipase - Ingenbosch_2021_J.Org.Chem__
Author(s) : Ingenbosch KN , Vieyto-Nunez JC , Ruiz-Blanco YB , Mayer C , Hoffmann-Jacobsen K , Sanchez-Garcia E
Ref : J Org Chem , : , 2021
Abstract :
PubMedSearch : Ingenbosch_2021_J.Org.Chem__
PubMedID: 34706196
Gene_locus related to this paper: bacsu-lip

Title : Rosetta-Enabled Structural Prediction of Permissive Loop Insertion Sites in Proteins - Plaks_2020_Biochemistry_59_3993
Author(s) : Plaks JG , Brewer JA , Jacobsen NK , McKenna M , Uzarski JR , Lawton TJ , Filocamo SF , Kaar JL
Ref : Biochemistry , 59 :3993 , 2020
Abstract :
PubMedSearch : Plaks_2020_Biochemistry_59_3993
PubMedID: 32970423
Gene_locus related to this paper: bacsu-lip

Title : Deletion and Randomization of Structurally Variable Regions in B. subtilis Lipase A (BSLA) Alter Its Stability and Hydrolytic Performance Against Long Chain Fatty Acid Esters - Martinez_2020_Int.J.Mol.Sci_21_
Author(s) : Martinez R , Bernal C , Alvarez R , Concha C , Araya F , Cabrera R , Dhoke GV , Davari MD
Ref : Int J Mol Sci , 21 : , 2020
Abstract :
PubMedSearch : Martinez_2020_Int.J.Mol.Sci_21_
PubMedID: 32183336
Gene_locus related to this paper: bacsu-lip

Title : Thermostability of Lipase A and Dynamic Communication Based on Residue Interaction Network - Xia_2019_Protein.Pept.Lett_26_702
Author(s) : Xia Q , Ding Y
Ref : Protein Pept Lett , 26 :702 , 2019
Abstract :
PubMedSearch : Xia_2019_Protein.Pept.Lett_26_702
PubMedID: 31215367
Gene_locus related to this paper: bacsu-lip

Title : Site-directed mutagenesis: role of lid region for T1 lipase specificity - Mohamed_2018_Protein.Eng.Des.Sel_31_221
Author(s) : Mohamed RA , Salleh AB , Leow ATC , Yahaya NM , Abdul Rahman MB
Ref : Protein Engineering Des Sel , 31 :221 , 2018
Abstract :
PubMedSearch : Mohamed_2018_Protein.Eng.Des.Sel_31_221
PubMedID: 30239965
Gene_locus related to this paper: bacsu-lip

Title : Fluorescence spectroscopic analysis of the structure and dynamics of Bacillus subtilis lipase A governing its activity profile under alkaline conditions - Kubler_2015_Eur.Biophys.J_44_655
Author(s) : Kubler D , Ingenbosch KN , Bergmann A , Weidmann M , Hoffmann-Jacobsen K
Ref : Eur Biophysical Journal , 44 :655 , 2015
Abstract :
PubMedSearch : Kubler_2015_Eur.Biophys.J_44_655
PubMedID: 26224303
Gene_locus related to this paper: bacsu-lip

Title : Structural Rigidity and Protein Thermostability in Variants of Lipase A from Bacillus subtilis - Rathi_2015_PLoS.One_10_e0130289
Author(s) : Rathi PC , Jaeger KE , Gohlke H
Ref : PLoS ONE , 10 :e0130289 , 2015
Abstract :
PubMedSearch : Rathi_2015_PLoS.One_10_e0130289
PubMedID: 26147762
Gene_locus related to this paper: bacsu-lip

Title : Crystallographic Investigation of Imidazolium Ionic Liquid Effects on Enzyme Structure - Nordwald_2015_Chembiochem_16_2456
Author(s) : Nordwald EM , Plaks JG , Snell JR , Sousa MC , Kaar JL
Ref : Chembiochem , 16 :2456 , 2015
Abstract :
PubMedSearch : Nordwald_2015_Chembiochem_16_2456
PubMedID: 26388426
Gene_locus related to this paper: bacsu-lip

Title : Biophysical characterization of mutants of Bacillus subtilis lipase evolved for thermostability: factors contributing to increased activity retention - Augustyniak_2012_Protein.Sci_21_487
Author(s) : Augustyniak W , Brzezinska AA , Pijning T , Wienk H , Boelens R , Dijkstra BW , Reetz MT
Ref : Protein Science , 21 :487 , 2012
Abstract :
PubMedSearch : Augustyniak_2012_Protein.Sci_21_487
PubMedID: 22267088
Gene_locus related to this paper: bacsu-lip

Title : Integrating In Silico and In vitro approaches to dissect the stereoselectivity of Bacillus subtilis lipase A toward ketoprofen vinyl ester - Ni_2011_Chem.Biol.Drug.Des_78_301
Author(s) : Ni Z , Zhou P , Jin X , Lin XF
Ref : Chemical Biology Drug Des , 78 :301 , 2011
Abstract :
PubMedSearch : Ni_2011_Chem.Biol.Drug.Des_78_301
PubMedID: 21477088
Gene_locus related to this paper: bacsu-lip

Title : Complete genome sequence of Bacillus subtilis BSn5, an endophytic bacterium of Amorphophallus konjac with antimicrobial activity for the plant pathogen Erwinia carotovora subsp. carotovora - Deng_2011_J.Bacteriol_193_2070
Author(s) : Deng Y , Zhu Y , Wang P , Zhu L , Zheng J , Li R , Ruan L , Peng D , Sun M
Ref : Journal of Bacteriology , 193 :2070 , 2011
Abstract :
PubMedSearch : Deng_2011_J.Bacteriol_193_2070
PubMedID: 21317323
Gene_locus related to this paper: bacpu-pnbae , bacsu-CAH , bacsu-lip , bacsu-LIPB , bacsu-PPSE , bacsu-YBAC , bacsu-YDEN , bacsu-YTPA , bacsu-ytxm , bacsu-YVAK

Title : In vitro evolved non-aggregating and thermostable lipase: structural and thermodynamic investigation - Kamal_2011_J.Mol.Biol_413_726
Author(s) : Kamal MZ , Ahmad S , Molugu TR , Vijayalakshmi A , Deshmukh MV , Sankaranarayanan R , Rao NM
Ref : Journal of Molecular Biology , 413 :726 , 2011
Abstract :
PubMedSearch : Kamal_2011_J.Mol.Biol_413_726
PubMedID: 21925508
Gene_locus related to this paper: bacsu-lip

Title : Whole genome assembly of a natto production strain Bacillus subtilis natto from very short read data - Nishito_2010_BMC.Genomics_11_243
Author(s) : Nishito Y , Osana Y , Hachiya T , Popendorf K , Toyoda A , Fujiyama A , Itaya M , Sakakibara Y
Ref : BMC Genomics , 11 :243 , 2010
Abstract :
PubMedSearch : Nishito_2010_BMC.Genomics_11_243
PubMedID: 20398357
Gene_locus related to this paper: bacsu-CAH , bacsu-lip , bacsu-LIPB , bacsu-pnbae , bacsu-YBAC , bacsu-YDEN , bacsu-ydjp , bacsu-yfhM , bacsu-yisY , bacsu-YJCH , bacsu-YTMA , bacsu-YTPA , bacsu-ytxm , bacsu-YUII , bacsu-YVAK , bacsu-RsbQ

Title : Loop grafting of Bacillus subtilis lipase A: inversion of enantioselectivity - Boersma_2008_Chem.Biol_15_782
Author(s) : Boersma YL , Pijning T , Bosma MS , van der Sloot AM , Godinho LF , Droge MJ , Winter RT , van Pouderoyen G , Dijkstra BW , Quax WJ
Ref : Chemical Biology , 15 :782 , 2008
Abstract :
PubMedSearch : Boersma_2008_Chem.Biol_15_782
PubMedID: 18721749
Gene_locus related to this paper: bacsu-lip

Title : Thermostable Bacillus subtilis lipases: in vitro evolution and structural insight - Ahmad_2008_J.Mol.Biol_381_324
Author(s) : Ahmad S , Kamal MZ , Sankaranarayanan R , Rao NM
Ref : Journal of Molecular Biology , 381 :324 , 2008
Abstract :
PubMedSearch : Ahmad_2008_J.Mol.Biol_381_324
PubMedID: 18599073
Gene_locus related to this paper: bacsu-lip

Title : Structural basis for the remarkable stability of Bacillus subtilis lipase (Lip A) at low pH - Rajakumara_2008_Biochim.Biophys.Acta_1784_302
Author(s) : Rajakumara E , Acharya P , Ahmad S , Sankaranaryanan R , Rao NM
Ref : Biochimica & Biophysica Acta , 1784 :302 , 2008
Abstract :
PubMedSearch : Rajakumara_2008_Biochim.Biophys.Acta_1784_302
PubMedID: 18053819
Gene_locus related to this paper: bacsu-lip

Title : Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: crystal structures and phage display selection - Droge_2006_Chembiochem_7_149
Author(s) : Droge MJ , Boersma YL , van Pouderoyen G , Vrenken TE , Ruggeberg CJ , Reetz MT , Dijkstra BW , Quax WJ
Ref : Chembiochem , 7 :149 , 2006
Abstract :
PubMedSearch : Droge_2006_Chembiochem_7_149
PubMedID: 16342303
Gene_locus related to this paper: bacsu-lip

Title : Crystallization and preliminary X-ray crystallographic investigations on several thermostable forms of a Bacillus subtilis lipase - Rajakumara_2004_Acta.Crystallogr.D.Biol.Crystallogr_60_160
Author(s) : Rajakumara E , Acharya P , Ahmad S , Shanmugam VM , Rao NM , Sankaranarayanan R
Ref : Acta Crystallographica D Biol Crystallogr , 60 :160 , 2004
Abstract :
PubMedSearch : Rajakumara_2004_Acta.Crystallogr.D.Biol.Crystallogr_60_160
PubMedID: 14684916
Gene_locus related to this paper: bacsu-lip

Title : Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase - Acharya_2004_J.Mol.Biol_341_1271
Author(s) : Acharya P , Rajakumara E , Sankaranarayanan R , Rao NM
Ref : Journal of Molecular Biology , 341 :1271 , 2004
Abstract :
PubMedSearch : Acharya_2004_J.Mol.Biol_341_1271
PubMedID: 15321721
Gene_locus related to this paper: bacsu-lip

Title : Alternate conformations observed in catalytic serine of Bacillus subtilis lipase determined at 1.3 A resolution - Kawasaki_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1168
Author(s) : Kawasaki K , Kondo H , Suzuki M , Ohgiya S , Tsuda S
Ref : Acta Crystallographica D Biol Crystallogr , 58 :1168 , 2002
Abstract :
PubMedSearch : Kawasaki_2002_Acta.Crystallogr.D.Biol.Crystallogr_58_1168
PubMedID: 12077437
Gene_locus related to this paper: bacsu-lip

Title : Lipolytic enzymes LipA and LipB from Bacillus subtilis differ in regulation of gene expression, biochemical properties, and three-dimensional structure - Eggert_2001_FEBS.Lett_502_89
Author(s) : Eggert T , van Pouderoyen G , Dijkstra BW , Jaeger KE
Ref : FEBS Letters , 502 :89 , 2001
Abstract :
PubMedSearch : Eggert_2001_FEBS.Lett_502_89
PubMedID: 11583117
Gene_locus related to this paper: bacsu-lip , bacsu-LIPB

Title : The crystal structure of Bacillus subtilis lipase: a minimal alpha\/beta hydrolase fold enzyme - van Pouderoyen_2001_J.Mol.Biol_309_215
Author(s) : van Pouderoyen G , Eggert T , Jaeger KE , Dijkstra BW
Ref : Journal of Molecular Biology , 309 :215 , 2001
Abstract :
PubMedSearch : van Pouderoyen_2001_J.Mol.Biol_309_215
PubMedID: 11491291
Gene_locus related to this paper: bacsu-lip

Title : A 32 kb nucleotide sequence from the region of the lincomycin-resistance gene (22 degrees-25 degrees) of the Bacillus subtilis chromosome and identification of the site of the lin-2 mutation - Kumano_1997_Microbiology_143_2775
Author(s) : Kumano M , Tamakoshi A , Yamane K
Ref : Microbiology , 143 ( Pt 8) :2775 , 1997
Abstract :
PubMedSearch : Kumano_1997_Microbiology_143_2775
PubMedID: 9274031
Gene_locus related to this paper: bacsu-lip

Title : The complete genome sequence of the gram-positive bacterium Bacillus subtilis - Kunst_1997_Nature_390_249
Author(s) : Kunst F , Ogasawara N , Moszer I , Albertini AM , Alloni G , Azevedo V , Bertero MG , Bessieres P , Bolotin A , Borchert S , Borriss R , Boursier L , Brans A , Braun M , Brignell SC , Bron S , Brouillet S , Bruschi CV , Caldwell B , Capuano V , Carter NM , Choi SK , Cordani JJ , Connerton IF , Cummings NJ , Daniel RA , Denziot F , Devine KM , Dusterhoft A , Ehrlich SD , Emmerson PT , Entian KD , Errington J , Fabret C , Ferrari E , Foulger D , Fritz C , Fujita M , Fujita Y , Fuma S , Galizzi A , Galleron N , Ghim SY , Glaser P , Goffeau A , Golightly EJ , Grandi G , Guiseppi G , Guy BJ , Haga K , Haiech J , Harwood CR , Henaut A , Hilbert H , Holsappel S , Hosono S , Hullo MF , Itaya M , Jones L , Joris B , Karamata D , Kasahara Y , Klaerr-Blanchard M , Klein C , Kobayashi Y , Koetter P , Koningstein G , Krogh S , Kumano M , Kurita K , Lapidus A , Lardinois S , Lauber J , Lazarevic V , Lee SM , Levine A , Liu H , Masuda S , Mauel C , Medigue C , Medina N , Mellado RP , Mizuno M , Moestl D , Nakai S , Noback M , Noone D , O'Reilly M , Ogawa K , Ogiwara A , Oudega B , Park SH , Parro V , Pohl TM , Portelle D , Porwollik S , Prescott AM , Presecan E , Pujic P , Purnelle B , Rapoport G , Rey M , Reynolds S , Rieger M , Rivolta C , Rocha E , Roche B , Rose M , Sadaie Y , Sato T , Scanlan E , Schleich S , Schroeter R , Scoffone F , Sekiguchi J , Sekowska A , Seror SJ , Serror P , Shin BS , Soldo B , Sorokin A , Tacconi E , Takagi T , Takahashi H , Takemaru K , Takeuchi M , Tamakoshi A , Tanaka T , Terpstra P , Togoni A , Tosato V , Uchiyama S , Vandebol M , Vannier F , Vassarotti A , Viari A , Wambutt R , Wedler H , Weitzenegger T , Winters P , Wipat A , Yamamoto H , Yamane K , Yasumoto K , Yata K , Yoshida K , Yoshikawa HF , Zumstein E , Yoshikawa H , Danchin A
Ref : Nature , 390 :249 , 1997
Abstract :
PubMedSearch : Kunst_1997_Nature_390_249
PubMedID: 9384377
Gene_locus related to this paper: bacsu-CAH , bacsu-cbxnp , bacsu-lip , bacsu-LIPB , bacsu-PKSR , bacsu-pnbae , bacsu-PPSE , bacsu-srf4 , bacsu-srfac , bacsu-YBAC , bacsu-YBDG , bacsu-ybfk , bacsu-ycgS , bacsu-yczh , bacsu-YDEN , bacsu-ydjp , bacsu-yfhM , bacsu-yisY , bacsu-YITV , bacsu-yjau , bacsu-YJCH , bacsu-MHQD , bacsu-yqjl , bacsu-yqkd , bacsu-YRAK , bacsu-YTAP , bacsu-YTMA , bacsu-YTPA , bacsu-ytxm , bacsu-yugF , bacsu-YUII , bacsu-YUKL , bacsu-YVAK , bacsu-YvaM , bacsu-RsbQ

Title : Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme - Lesuisse_1993_Eur.J.Biochem_216_155
Author(s) : Lesuisse E , Schanck K , Colson C
Ref : European Journal of Biochemistry , 216 :155 , 1993
Abstract :
PubMedSearch : Lesuisse_1993_Eur.J.Biochem_216_155
PubMedID: 8396026
Gene_locus related to this paper: bacsu-lip

Title : Cloning, nucleotide sequence and expression in Escherichia coli of a lipase gene from Bacillus subtilis 168 - Dartois_1992_Biochim.Biophys.Acta_1131_253
Author(s) : Dartois V , Baulard A , Schanck K , Colson C
Ref : Biochimica & Biophysica Acta , 1131 :253 , 1992
Abstract :
PubMedSearch : Dartois_1992_Biochim.Biophys.Acta_1131_253
PubMedID: 1320940
Gene_locus related to this paper: bacsu-lip