Fersini_2012_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_68_923

Reference

Title : Cloning, expression, purification and preliminary X-ray analysis of a putative metagenome-derived lipase - Fersini_2012_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_68_923
Author(s) : Fersini F , Dall'Antonia Y , Chow J , Streit WR , Mueller-Dieckmann J
Ref : Acta Crystallographica Sect F Struct Biol Cryst Commun , 68 :923 , 2012
Abstract :

LipS is a novel thermostable putative lipase that was isolated from a metagenomic library using functional screening methods. The corresponding gene shows high similarity to that encoding a putative but uncharacterized esterase from Symbiobacterium thermophilum IAM14863 (99% nucleotide-sequence similarity). Two different constructs of the recombinant lipase were crystallized. Crystals belonging to space group P4(2)2(1)2 diffracted X-ray radiation to 2.8 A resolution and crystals belonging to space group P4 diffracted to 2.0 A resolution. The most probable content of their asymmetric units were two molecules (P4(2)2(1)2) and four or five molecules (P4), respectively.

PubMedSearch : Fersini_2012_Acta.Crystallogr.Sect.F.Struct.Biol.Cryst.Commun_68_923
PubMedID: 22869123
Gene_locus related to this paper: symth-q67mr3

Related information

Inhibitor Spermidine
Gene_locus Spermidine    symth-q67mr3
Family Spermidine    symth-q67mr3    CarbLipBact_1    CarbLipBact_2
Structure Spermidine    symth-q67mr3    CarbLipBact_1    CarbLipBact_2    4FBL    4FBM

Citations formats

Fersini F, Dall'Antonia Y, Chow J, Streit WR, Mueller-Dieckmann J (2012)
Cloning, expression, purification and preliminary X-ray analysis of a putative metagenome-derived lipase
Acta Crystallographica Sect F Struct Biol Cryst Commun 68 :923

Fersini F, Dall'Antonia Y, Chow J, Streit WR, Mueller-Dieckmann J (2012)
Acta Crystallographica Sect F Struct Biol Cryst Commun 68 :923