Filho_2012_Phytother.Res_26_1472

The phenylpropanoid glycoside verbascoside 2-(3,4-dihydroxyphenylethyl)-1-O-alpha-l-rhamnopyranosyl-(1-->3)-beta-d-(4-O-caf feyl)-glucopyranoside 1 has been isolated as the main constituent of the crude extract of Buddleja brasiliensis Jacq ex Spreng from Southern Brazil The crude extract main fractions and the compound 1 were evaluated for inhibition of the enzymes acetylcholinesterase AChE dipeptidyl peptidase-IV DPP-IV and prolyl oligopeptidase POP Compound 1 showed weak activity against DPP-IV with an IC(50 150 microm and was inactive against AChE with a pMIQ determined by bioautography of 9.6 In contrast 1 displayed significant inhibition of POP in a dose-dependent manner with an IC(50 value of 1.3 0.2 microm similar to the positive control baicalin with a POP IC(50 of 12 3 microm