Title : Characterization of Cinnamoyl Esterases from Different Lactobacilli and Bifidobacteria - Fritsch_2017_Curr.Microbiol_74_247
Author(s) : Fritsch C , Jansch A , Ehrmann MA , Toelstede S , Vogel RF
Ref : Curr Microbiol , 74 :247 , 2017
Abstract :

A high variety of plants that are used for food production contain esterified hydroxycinnamic acids. As their free forms display several benefits, like an enhanced absorption in human intestinal tract, anti-oxidative and anti-carcinogenic effects, an improved protein solubility and reduced discoloration, the microbial ability to cleave the ester bond is highly desired. In order to examine potential fermentation strains for this purpose, six different lactic acid bacteria and one bifidobacterial strain were screened for their ability to degrade esterified hydroxycinnamic acids because these strains are commonly used for fermentation of plant-based foods. Moreover, their cinnamoyl esterase activity was examined by molecular biological analyses. The enzymes were heterologously expressed in Escherichia coli, purified and biochemically characterized. The purified esterases with a molecular mass around 27-29 kDa had their optimum predominantly between pH 7 and 8 at 20-30 degreesC. Bifidobacterium animalis subsp. lactis, Lactobacillus gasseri, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus fermentum displayed activities against a broad substrate range (methyl caffeate, methyl trans-p-coumarate, chlorogenic acid as well as partially ethyl ferulate). Concerning substrate affinity, reaction velocity, thermal and pH stability, Lactobacillus gasseri showed the overall best performance. The herein studied lactic acid- and bifidobacteria are promising for the production of fermented plant-based foods with an increased quality and nutritional value.

PubMedSearch : Fritsch_2017_Curr.Microbiol_74_247
PubMedID: 27999938
Gene_locus related to this paper: limf3-b2gdc2 , lache-u6f2k7 , bifa0-b8du06 , lacac-q5fi30 , lacga-q040s2 , lacpl-LP.2953 , lacre-q2bvr2

Related information

Substrate Ara1F    FAXG    Chlorogenic-acid    Ethyl-ferulate
Gene_locus Ara1F    FAXG    Chlorogenic-acid    Ethyl-ferulate    limf3-b2gdc2    lache-u6f2k7    bifa0-b8du06    lacac-q5fi30    lacga-q040s2    lacpl-LP.2953    lacre-q2bvr2
Family Ara1F    FAXG    Chlorogenic-acid    Ethyl-ferulate    limf3-b2gdc2    lache-u6f2k7    bifa0-b8du06    lacac-q5fi30    lacga-q040s2    lacpl-LP.2953    lacre-q2bvr2    FAE-Bacterial-promiscuous

Citations formats

Fritsch C, Jansch A, Ehrmann MA, Toelstede S, Vogel RF (2017)
Characterization of Cinnamoyl Esterases from Different Lactobacilli and Bifidobacteria
Curr Microbiol 74 :247

Fritsch C, Jansch A, Ehrmann MA, Toelstede S, Vogel RF (2017)
Curr Microbiol 74 :247