Family: FAE-Bacterial-promiscuous

Block: X

Parent Family: No family


Family extracted from the large AlphaBeta hydrolase family. Promiscuous Feruloyl Esterase (Est1E) from the Rumen Bacterium Butyrivibrio Proteoclasticus structure reveals a new lid domain. Polyethylene-terephthalate hydrolases. Pinotsis et al. shows that this family also contains non-canonical prototype long-chain monoacylglycerol lipases


Interpro : IPR053145 AB hydrolase superfamily Esterase 10 AB_hydrolase_Est10


Pdoc : No Pdoc

Pfam : No Pfam

Prints : No Print

EC Number : No EC Number


Peptide in Fasta
Nucleotide in Fasta
Alignment with Multalin Text only
Seed alignment with MAFFT No colour / coloured with Mview
Alignment with MAFFT No colour / coloured with Mview
Dendrogram The dnd file

Structure Name Proteins
8B4U The crystal structure of PET46 archaeal lid-containing feruloyl-esterase PETase 9arch-PET46
8SLJ K164A mutant of a chlorogenic acid esterase from Lactobacillus helveticus lache-u6f2k7
8SKM Wild type chlorogenic acid esterase from Lactobacillus helveticus lache-u6f2k7
7Z2V Ferulic acid esterase variant S114A from Lactobacillus buchneri lenbu-d7ru28
7Z2X Wild-type ferulic acid esterase from Lactobacillus buchneri lenbu-d7ru28
7Z2U Wild-type ferulic acid esterase from Lactobacillus buchneri in complex with ferulate lenbu-d7ru28
7WWH Crystal structure of the Geobacillus thermoglucosidasius feruloyl esterase GthFAE partm-GthFAE
7Q4H A thermostable lipase from Thermoanaerobacter thermohydrosulfuricus in complex with PMSF theet-q3ch51
8B9S A thermostable lipase from Thermoanaerobacter thermohydrosulfuricus native form theet-q3ch51
7Q4J A thermostable lipase from Thermoanaerobacter thermohydrosulfuricus in complex a monoacylglycerol intermediate theet-q3ch51
7XRH Crystal structure of ferulic acid esterase (LaFae) from Lactobacillus acidophilus (7DOS withdrawn) lacac-q5fi30
7XRI Crystal structure of ferulic acid esterase (LaFae) from Lactobacillus acidophilus mutant -S106A (7DOT withdrawn) complex with ethyl ferulate lacac-q5fi30
3PF8 Crystal structure of the Lactobacillus johnsonii cinnamoyl esterase LJ0536 lacjo-q74hk5
3PF9 Crystal structure of the Lactobacillus johnsonii cinnamoyl esterase LJ0536 S106A mutant lacjo-q74hk5
3PFB Crystal structure of the Lactobacillus johnsonii cinnamoyl esterase LJ0536 S106A mutant in complex with ethylferulate lacjo-q74hk5
3PFC Crystal structure of the Lactobacillus johnsonii cinnamoyl esterase LJ0536 S106A mutant in complex with ferulic acid lacjo-q74hk5
3QM1 Crystal structure of the Lactobacillus johnsonii cinnamoyl esterase LJ0536 S106A mutant in complex with ethylferulate, Form II lacjo-q74hk5
3S2Z Crystal structure of the Lactobacillus johnsonii cinnamoyl esterase LJ0536 S106A mutant in complex with caffeic acid (replaced 3PFA: withdrawn) lacjo-q74hk5
2WTM Promiscuous Feruloyl Esterase (Est1E) from the Rumen Bacterium Butyrivibrio Proteoclasticus. 9firm-Est1E
2WTN Ferulic acid bound Promiscuous Feruloyl Esterase (Est1E) from the Rumen Bacterium Butyrivibrio Proteoclasticus. 9firm-Est1E

Gene_locus Name Species
lacam-a0a1c9u7k7 Lactobacillus amylovorus Feruloyl esterase Lactobacillus amylovorus
9clot-a0a3r6ruf5 Clostridiaceae bacterium AF18-31LB Alpha\/beta fold hydrolase HG-3 PET-hydrolase Clostridiaceae bacterium AF18-31LB
9firm-a0a413uca3 Holdemanella biformis Alpha\/beta fold hydrolase HG-2 PET-hydrolase Holdemanella biformis
9arch-PETcan211 Candidatus Bathyarchaeota archaeon MAG: alpha\/beta fold hydrolase Candidatus Bathyarchaeota archaeon
9cren-PETcan204 Thermofilum adornatum alpha\/beta fold hydrolase PETcan204 Thermofilum adornatum
9arch-PET46 Candidatus Bathyarchaeota archaeon, archaeal lid-containing feruloyl-esterase, polyethylene terephthalate hydrolase (PET)PET46 PETase\/FAE Candidatus Bathyarchaeota archaeon
limf3-b2gdc2 Limosilactobacillus fermentum (strain NBRC 3956 \/ LMG 18251) (Lactobacillus fermentum) ferulic acid esterase Limosilactobacillus fermentum IFO 3956
lache-u6f2k7 Lactobacillus helveticus highly active esterase. hydrolyzes chlorogenic acid Lactobacillus helveticus
lenbu-d7ru28 Lentilactobacillus buchneri (Lactobacillus buchneri) Ferulic acid esterase faeA LbFAE Lentilactobacillus buchneri
partm-GthFAE Parageobacillus thermoglucosidasius (Geobacillus thermoglucosidasius) strain DSM 2542 feruloyl esterase Parageobacillus thermoglucosidasius
bifa0-b8du06 Bifidobacterium animalis subsp. Alpha\/beta hydrolase fold family protein lactis BLA_1197 Balat_0669 Bifidobacterium animalis subsp. lactis
geoth-g8myf3 Geobacillus thermoleovorans EH68 Uncharacterized protein Geobacillus thermoleovorans CCB_US3_UF5
9zzzz-KY203030 uncultured prokaryote, EH31 esterase\/lipase uncultured prokaryote
9baci-q2bay3 Bacillus sp. NRRL B-14911 alpha\/beta superfamily hydrolase Bacillus sp. NRRL B-14911
9firm-Est1E Butyrivibrio proteoclasticus (strain ATCC 51982 \/ DSM 14932 \/ B316) (Clostridium proteoclasticum) Promiscuous Feruloyl Esterase (Est1E) from the Rumen Bacterium Butyrivibrio Proteoclasticus B316
butfi-CINI Butyrivibrio fibrisolvens cinnamoyl ester hydrolase cinI Butyrivibrio fibrisolvens
butfi-CINII Butyrivibrio fibrisolvens cinii Butyrivibrio fibrisolvens
cloac-CAC3665 Clostridium acetobutylicum alpha\/beta superfamily hydrolase Clostridium acetobutylicum
deira-DR1053 Deinococcus radiodurans hydrolase, putative Deinococcus radiodurans
frasc-q2j9z1 Frankia sp. (strain CcI3) alpha\/beta hydrolase fold Frankia sp. CcI3
frasc-q2jel7 Frankia sp. (strain CcI3) alpha\/beta hydrolase fold Frankia sp. CcI3
ignaa-e0snk2 Ignisphaera aggregans strain (strain DSM 17230 \/ JCM 13409 \/ AQ1.S1) Dienelactone hydrolase Ignisphaera aggregans
lacac-q5fi30 Lactobacillus acidophilus ferulic acid esterase (LaFae) Lactobacillus acidophilus
lacac-q5fje7 Lactobacillus acidophilus putative alpha-beta superfamily hydrolase Lactobacillus acidophilus
lacga-q040s2 Lactobacillus gasseri (strain ATCC 33323 \/ DSM 20243) alpha\/beta superfamily hydrolase Lactobacillus gasseri
lacjo-q74hk5 Lactobacillus johnsonii NCC 533 Hydrolase_4 domain-containing protein cinnamoyl esterase Lactobacillus johnsonii NCC 533
lacjo-q74j82 Lactobacillus johnsonii NCC 533 hypothetical protein Lactobacillus johnsonii NCC 533
lacpl-LP.2953 Lactobacillus plantarum esterase (putative) Lactobacillus plantarum
lacre-q2bvr2 Lactobacillus reuteri JCM 1112 and 100-23 hypothetical protein Lactobacillus reuteri JCM 1112
meslo-MLR6657 Rhizobium loti (Mesorhizobium loti) hypothetical protein mlr6657 Rhizobium loti
parde-O54025 Paracoccus denitrificans (and PD1222) orf2 protein Paracoccus denitrificans
paruw-q6mcu6 Parachlamydia sp. subsp. Acanthamoeba sp. (strain UWE25) hypothetical protein Candidatus Protochlamydia amoebophila UWE25
theet-q3ch51 Thermoanaerobacter thermohydrosulfuricus\; Thermoanaerobacter ethanolicus TTL lipase LipTth Thermoanaerobacter ethanolicus ATCC 33223
thete-TTE1809 Thermoanaerobacter tengcongensis Caldanaerobacter subterraneus subsp. tengcongensis Thermostable lipase LipCst Thermoanaerobacter tengcongensis
trede-q73qt5 Treponema denticola cinnamoyl ester hydrolase Treponema denticola
dict6-b5yfh9 Dictyoglomus thermophilum (strain ATCC 35947 \/ DSM 3960 \/ H-6-12). EstDZ3 Hydrolases of the alpha\/beta superfamily Dictyoglomus thermophilum
dictd-b8e2k8 Dictyoglomus turgidum (strain Z-1310 \/ DSM 6724). Dienelactone hydrolase Dictyoglomus turgidum
9arch-a0a0n8pwm8 Candidatus Bathyarchaeota archaeon BA1. Esterase Candidatus Bathyarchaeota archaeon BA1
wadcw-d6ywc6 Waddlia chondrophila. Uncharacterized protein Waddlia chondrophila
9chla-a0a0c1h3n5 Candidatus Protochlamydia amoebophila. Uncharacterized protein Candidatus Protochlamydia amoebophila
9chla-a0a078l8a9 Candidatus Rubidus massiliensis. Fermentation\/respiration switch protein Candidatus Rubidus massiliensis
parav-f8kzh9 Parachlamydia acanthamoebae Uncharacterized protein Parachlamydia acanthamoebae
9bacl-a0a0p9gq96 Alicyclobacillus ferrooxydans. Alpha\/beta hydrolase Alicyclobacillus ferrooxydans
9chla-a0a090cyn4 Criblamydia sequanensis CRIB-18. Uncharacterized protein Criblamydia sequanensis CRIB-18
9bact-h8xw45 uncultured bacterium. Carboxylesterase uncultured bacterium
9chla-a0a0u5jg21 Protochlamydia naegleriophila. Uncharacterized protein Protochlamydia naegleriophila
aceaz-d9qqr0 Acetohalobium arabaticum (strain ATCC 49924 \/ DSM 5501 \/ Z-7288). BAAT\/Acyl-CoA thioester hydrolase Acetohalobium arabaticum
9baci-a0a023dfm7 Parageobacillus caldoxylosilyticus NBRC 107762 (Geobacillus caldoxylosilyticus NBRC 107762). Hydrolase_4 domain-containing protein Geobacillus caldoxylosilyticus NBRC 107762
9chla-a0a0h5dp67 Estrella lausannensis. Uncharacterized protein Estrella lausannensis
9bact-a0a0g2zfb4 Kosmotoga pacifica. Uncharacterized protein Kosmotoga pacifica
9bact-estC76.221 uncultured bacterium. Lipolytic protein uncultured bacterium

Gene_locus_frgt Name Species
9firm-a0a139clv2 Halobacteroidaceae bacterium T328-2. BAAT\/Acyl-CoA thioester hydrolase Halobacteroidaceae bacterium T328-2

Substrate Chemical Nomenclature Proteins
Coumarin-3-carboxylic-acid 2-oxochromene-3-carboxylic acid 9firm-Est1E
Cinnamic-acid (E)-3-phenylprop-2-enoic acid 9firm-Est1E    canar-LipB    humla-1lipa
Ethyl-cinnamate ethyl (E)-3-phenylprop-2-enoate 9firm-Est1E    humla-1lipa    9arch-PET46
Ethyl-coumarin-3-carboxylate ethyl 2-oxochromene-3-carboxylate 9firm-Est1E
Monostearin 2,3-dihydroxypropyl octadecanoate mouse-Ces2c    human-CES2    theet-q3ch51
Ferulic-acid (E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid clotm-xynz    lacjo-q74hk5    lenbu-d7ru28    aspni-FAEA    aspng-faeb
MHET 4-(2-hydroxyethoxycarbonyl)benzoic acid idesa-peth    idesa-mheth    thefu-q6a0i3    thefu-q6a0i4    thefu-1831    bacsu-pnbae    fusso-cutas    9arch-PET46    thema-ESTA
BHET bis(2-hydroxyethyl) benzene-1,4-dicarboxylate idesa-peth    idesa-mheth    thefu-q6a0i3    thefu-q6a0i4    9flao-a0a330mq60    9flao-a0a0c1f4u8    9flao-kjj39608    9flao-a0a1m6f5v0    thefu-1831    fusso-cutas    9bact-EH0    theck-l0ef70    9arch-PET46    9flao-ChryBHETase    bacsu-pnbae    exiab-k0afs0    9bact-g9by57    thema-ESTA    9bact-a0a2h5z9r5    9flao-MarCE    strco-LIPA
Polyethylene-terephthalate 2-methoxyethyl 4-acetylbenzoate idesa-peth    thefu-q6a0i4    9acto-f7ix06    9bact-g9by57    sacvd-c7mve8    thecd-d1a9g5    9actn-h6wx58    9zzzz-A0A0G3FEJ8    thefu-q6a0i3    9flao-a0a330mq60    9flao-a0a0c1f4u8    bacsu-pnbae    9zzzz-Ple628    9zzzz-Ple629    9firm-PHL7    9bact-c3ryl0    9gamm-PETaseMG8    olean-r4ykl9    morsp-lip1    vibga-a0a1z2siq1    vibga-a0a1m5fok3    pseol-e9kjl1    9gamm-PpelaLip    9psed-peh    9pseu-a0a1i6nu60    thecd-d1a2h1    9actn-a0a147kjy8    9bact-102Pee006    9cyan-305pEE028    9chlr-7QJM202    9bact-a0a656d8b6    fusso-cutas    thefu-1831    9actn-a0a1t4kk94    humin-cut    aspor-cutas    9burk-a0a1f4jxw8    9burk-PET11    thecs-711Erick    9actn-RII04304    9acto-d4q9n1    9gamm-a0a3l8bw54    9actn-a0a0n0my27    9burk-a0a1e4lw26    9actn-Alr407    9gamm-a0a3l8bdt3    9gamm-a0a2k9lit3    9actn-a0a0n0ney5    9actn-e9upm2    9arch-PET46    psea4-u2z2l5    9gamm-a0a031mkr8    9burk-a0a1w6l438    9psed-a0a078mgg8    9burk-a0a0g3bi90    9burk-a0a1w6l588    acide-PBSA    9baci-a0a090kq36    9baci-a0a0d0eil0    9baci-a0a090izy6    9baci-a0a090ivg8    9baci-a0a0d0ejv8    entcl-j7gkj7    9firm-a0a413uca3    9clot-a0a3r6ruf5    9firm-a0a4r3svb5    heyc2-f7z2k4
Paranitrophenyl-ferulate (4-nitrophenyl) 3-(4-hydroxy-3-methoxyphenyl)prop-2-enoate aspni-FAEA    asptu-FAEA    partm-GthFAE    lenbu-d7ru28
Ethyl-ferulate ethyl (E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoate lacjo-q74hk5    biflo-BL0186    asptn-q0ci40    9firm-Est1E    neucr-FAED    lacac-q5fi30    lacga-q040s2    strcj-estA    partm-GthFAE    pyrca-PCEST
Methyl-ferulate methyl (E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoate 9bace-b3cet1    9bace-b3c9d0.2    biflo-BL0186    emeni-faec    asptn-q0ci40    neucr-FAED    asptu-FAEA    pench-q3v6c9    lenbu-d7ru28    partm-GthFAE    9zzzz-DAC80243    9zzzz-CE1.6RZN    pyrca-PCEST
1-Naphtylpropionate naphthalen-1-yl propanoate lacjo-q74hk5    aphgo-cxest    aspor-faec    aspor-q2uf27
1-Naphtylbutyrate naphthalen-1-yl butanoate lacjo-q74hk5    aspor-faec    aspor-q2uf27    pseae-PA3859    maggr-cutas
1-Naphtylacetate naphthalen-1-yl acetate burgl-EstC    human-BCHE    lacjo-q74hk5    aspor-faec    aspor-q2uf27    pseae-PA3859    aphgo-cxest
Paranitrophenyl-octanoate (4-nitrophenyl) octanoate 9firm-a0a0a0ymq9    9bact-Q0GMU2    psesp-OPH    human-NOTUM    pseae-PA2949    crysp-Q874E9    emeni-q5b2c1    fusso-cutas    thite-g2rae6    9flao-a0a330mq60    9flao-a0a0c1f4u8    malgo-a8puy1    9bact-6EB3    partm-GthFAE    9bact-a0a2h5z9r5    9actn-j7k890    9actn-j7kdc6    fusox-a0a1d3s5h0
Monooctanoin 2,3-dihydroxypropyl octanoate mouse-Ces2c    human-CES2    theet-q3ch51

Inhibitor Chemical Nomenclature Proteins
Caffeic-acid (E)-3-(3,4-dihydroxyphenyl)prop-2-enoic acid lacjo-q74hk5
PMSF Phenylmethylsulfonyl fluoride theet-q3ch51    emeni-q5avy9    human-ABHD10    human-PREPL    myctu-Rv1430    psefl-cxest    9bact-E40    thefu-q6a0i4    ecoli-Aes    orysj-Q10QA5    bac25-mglp    9bact-Q0GMU2    bacsu-FENB    psepu-clcd1    thema-TM0077    bacsu-RsbQ    ecoli-bioh    drome-CG9542    human-FAP    tubmm-TmEst2    human-NOTUM    cutac-e6dae5    9gamm-urc15994    9baci-EstD9    geost-est30    pholl-AntI

References (8)

Title : Discovery of a non-canonical prototype long-chain monoacylglycerol lipase through a structure-based endogenous reaction intermediate complex - Pinotsis_2023_Nat.Commun_14_7649
Author(s) : Pinotsis N , Kruger A , Tomas N , Chatziefthymiou SD , Litz C , Mortensen SA , Daffe M , Marrakchi H , Antranikian G , Wilmanns M
Ref : Nat Commun , 14 :7649 , 2023
Abstract :
PubMedSearch : Pinotsis_2023_Nat.Commun_14_7649
PubMedID: 38012138

Title : An archaeal lid-containing feruloyl esterase degrades polyethylene terephthalate - Perez-Garcia_2023_Commun.Chem_6_193
Author(s) : Perez-Garcia P , Chow J , Costanzi E , Gurschke M , Dittrich J , Dierkes RF , Molitor R , Applegate V , Feuerriegel G , Tete P , Danso D , Thies S , Schumacher J , Pfleger C , Jaeger KE , Gohlke H , Smits SHJ , Schmitz RA , Streit WR
Ref : Commun Chem , 6 :193 , 2023
Abstract :
PubMedSearch : Perez-Garcia_2023_Commun.Chem_6_193
PubMedID: 37697032
Gene_locus related to this paper: 9arch-PETcan211 , 9cren-PETcan204 , 9arch-PET46

Title : Crystal structure of the feruloyl esterase from Lentilactobacillus buchneri reveals a novel homodimeric state - Kasmaei_2022_Front.Microbiol_13_1050160
Author(s) : Kasmaei KM , Kalyani DC , Reichenbach T , Jimenez-Quero A , Vilaplana F , Divne C
Ref : Front Microbiol , 13 :1050160 , 2022
Abstract :
PubMedSearch : Kasmaei_2022_Front.Microbiol_13_1050160
PubMedID: 36569051
Gene_locus related to this paper: lenbu-d7ru28

Title : Structure-guided rational design of the Geobacillus thermoglucosidasius feruloyl esterase GthFAE to improve its thermostability - Yang_2022_Biochem.Biophys.Res.Commun_600_117
Author(s) : Yang W , Sun L , Dong P , Chen Y , Zhang H , Huang X , Wu L , Chen L , Jing D , Wu Y
Ref : Biochemical & Biophysical Research Communications , 600 :117 , 2022
Abstract :
PubMedSearch : Yang_2022_Biochem.Biophys.Res.Commun_600_117
PubMedID: 35219099
Gene_locus related to this paper: partm-GthFAE

Title : Biochemical characterization of a novel thermostable feruloyl esterase from Geobacillus thermoglucosidasius DSM 2542(T) - Ay Sal_2019_Mol.Biol.Rep_46_4385
Author(s) : Ay Sal F , Colak DN , Guler HI , Canakci S , Belduz AO
Ref : Mol Biol Rep , 46 :4385 , 2019
Abstract :
PubMedSearch : Ay Sal_2019_Mol.Biol.Rep_46_4385
PubMedID: 31201678
Gene_locus related to this paper: partm-GthFAE

Title : Characterization of Cinnamoyl Esterases from Different Lactobacilli and Bifidobacteria - Fritsch_2017_Curr.Microbiol_74_247
Author(s) : Fritsch C , Jansch A , Ehrmann MA , Toelstede S , Vogel RF
Ref : Curr Microbiol , 74 :247 , 2017
Abstract :
PubMedSearch : Fritsch_2017_Curr.Microbiol_74_247
PubMedID: 27999938
Gene_locus related to this paper: limf3-b2gdc2 , lache-u6f2k7 , bifa0-b8du06 , lacac-q5fi30 , lacga-q040s2 , lacpl-LP.2953 , lacre-q2bvr2

Title : An inserted alpha\/beta subdomain shapes the catalytic pocket of Lactobacillus johnsonii cinnamoyl esterase - Lai_2011_PLoS.One_6_e23269
Author(s) : Lai KK , Stogios PJ , Vu C , Xu X , Cui H , Molloy S , Savchenko A , Yakunin A , Gonzalez CF
Ref : PLoS ONE , 6 :e23269 , 2011
Abstract :
PubMedSearch : Lai_2011_PLoS.One_6_e23269
PubMedID: 21876742
Gene_locus related to this paper: lacjo-q74hk5

Title : Structural and functional characterization of a promiscuous feruloyl esterase (Est1E) from the rumen bacterium Butyrivibrio proteoclasticus - Goldstone_2010_Proteins_78_1457
Author(s) : Goldstone DC , Villas-Boas SG , Till M , Kelly WJ , Attwood GT , Arcus VL
Ref : Proteins , 78 :1457 , 2010
Abstract :
PubMedSearch : Goldstone_2010_Proteins_78_1457
PubMedID: 20058325
Gene_locus related to this paper: 9firm-Est1E