Title : Multiple conformations of catalytic serine and histidine in acetylxylan esterase at 0.90 A - Ghosh_2001_J.Biol.Chem_276_11159 |
Author(s) : Ghosh D , Sawicki M , Lala P , Erman M , Pangborn W , Eyzaguirre J , Gutierrez R , Jornvall H , Thiel DJ |
Ref : Journal of Biological Chemistry , 276 :11159 , 2001 |
Abstract :
Acetylxylan esterase (AXEII; 207 amino acids) from Penicillium purpurogenum has substrate specificities toward acetate esters of d-xylopyranose residues in xylan and belongs to a new class of alpha/beta hydrolases. The crystal structure of AXEII has been determined by single isomorphous replacement and anomalous scattering, and refined at 0.90- and 1.10-A resolutions with data collected at 85 K and 295 K, respectively. The tertiary structure consists of a doubly wound alpha/beta sandwich, having a central six-stranded parallel beta-sheet flanked by two parallel alpha-helices on each side. The catalytic residues Ser(90), His(187), and Asp(175) are located at the C-terminal end of the sheet, an exposed region of the molecule. The serine and histidine side chains in the 295 K structure show the frequently observed conformations in which Ser(90) is trans and the hydroxyl group is in the plane of the imidazole ring of His(187). However, the structure at 85 K displays an additional conformation in which Ser(90) side-chain hydroxyl is away from the plane of the imidazole ring of His(187). The His(187) side chain forms a hydrogen bond with a sulfate ion and adopts an altered conformation. The only other known hydrolase that has a similar tertiary structure is Fusarium solani cutinase. The exposed nature of the catalytic triad suggests that AXEII is a pure esterase, i.e. an alpha/beta hydrolase with specificity for nonlipidic polar substrates. |
PubMedSearch : Ghosh_2001_J.Biol.Chem_276_11159 |
PubMedID: 11134051 |
Gene_locus related to this paper: penpu-axylest |
Gene_locus | penpu-axylest |
Family | Acetylxylan_esterase Cutinase_like |
Structure | 1G66 |
Ghosh D, Sawicki M, Lala P, Erman M, Pangborn W, Eyzaguirre J, Gutierrez R, Jornvall H, Thiel DJ (2001)
Multiple conformations of catalytic serine and histidine in acetylxylan esterase at 0.90 A
Journal of Biological Chemistry
276 :11159
Ghosh D, Sawicki M, Lala P, Erman M, Pangborn W, Eyzaguirre J, Gutierrez R, Jornvall H, Thiel DJ (2001)
Journal of Biological Chemistry
276 :11159