| Title : Identification and Characterization of Two Novel Esterases from a Metagenomic Library - Gu_2015_Food.Sci.Technol.Res_21_649 |
| Author(s) : Gu X , Wang S , Wang SC , Zhao LX , Cao M , Feng Z |
| Ref : Food Science and Technology Research , 21 :649 , 2015 |
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Abstract :
Esterases are biocatalysts in food industry aimed for nutrition improvements, formation of flavor, and food fermentation. Two esterases EstGX1 and EstGX2 were identified based on function-based screening of a soil metagenomic cosmid library. Enzyme properties including optimum pH, optimal temperature, tolerance to organic solvents and metal ions were measured, respectively. The activity of EstGX2 could maintain about 40% after incubated at 99C for 55 min, and could be increased in presence of 15% ethanol. The unique properties of EstGX2, high thermostability and stability in the presence of several organic solvents, may make it a promising enzyme candidate in food industry. |
| PubMedSearch : Gu_2015_Food.Sci.Technol.Res_21_649 |
| PubMedID: |
| Gene_locus related to this paper: 9bact-estGX1 , 9bact-a0a0d5w6z2 |
| Gene_locus | 9bact-estGX1 9bact-a0a0d5w6z2 |
Gu X, Wang S, Wang SC, Zhao LX, Cao M, Feng Z (2015)
Identification and Characterization of Two Novel Esterases from a Metagenomic Library
Food Science and Technology Research
21 :649
Gu X, Wang S, Wang SC, Zhao LX, Cao M, Feng Z (2015)
Food Science and Technology Research
21 :649