Hiessl_2012_Appl.Environ.Microbiol_78_2874

Reference

Title : Involvement of two latex-clearing proteins during rubber degradation and insights into the subsequent degradation pathway revealed by the genome sequence of Gordonia polyisoprenivorans strain VH2 - Hiessl_2012_Appl.Environ.Microbiol_78_2874
Author(s) : Hiessl S , Schuldes J , Thurmer A , Halbsguth T , Broker D , Angelov A , Liebl W , Daniel R , Steinbuchel A
Ref : Applied Environmental Microbiology , 78 :2874 , 2012
Abstract :

The increasing production of synthetic and natural poly(cis-1,4-isoprene) rubber leads to huge challenges in waste management. Only a few bacteria are known to degrade rubber, and little is known about the mechanism of microbial rubber degradation. The genome of Gordonia polyisoprenivorans strain VH2, which is one of the most effective rubber-degrading bacteria, was sequenced and annotated to elucidate the degradation pathway and other features of this actinomycete. The genome consists of a circular chromosome of 5,669,805 bp and a circular plasmid of 174,494 bp with average GC contents of 67.0% and 65.7%, respectively. It contains 5,110 putative protein-coding sequences, including many candidate genes responsible for rubber degradation and other biotechnically relevant pathways. Furthermore, we detected two homologues of a latex-clearing protein, which is supposed to be a key enzyme in rubber degradation. The deletion of these two genes for the first time revealed clear evidence that latex-clearing protein is essential for the microbial utilization of rubber. Based on the genome sequence, we predict a pathway for the microbial degradation of rubber which is supported by previous and current data on transposon mutagenesis, deletion mutants, applied comparative genomics, and literature search.

PubMedSearch : Hiessl_2012_Appl.Environ.Microbiol_78_2874
PubMedID: 22327575
Gene_locus related to this paper: gorpv-h6mtj1 , gorpv-h6mzw9 , gorpv-h6n0e3 , gorpv-h6n0g6 , gorpv-h6n0p7 , gorpv-h6mts4 , 9acto-h0rbp9 , 9acto-h0rc96 , gorpv-h6mu09 , gorpv-h6mtj7 , gorpv-h6n352 , gorpv-h6mui9 , gorpv-h6n4w7

Related information

Gene_locus gorpv-h6mtj1    gorpv-h6mzw9    gorpv-h6n0e3    gorpv-h6n0g6    gorpv-h6n0p7    gorpv-h6mts4    9acto-h0rbp9    9acto-h0rc96    gorpv-h6mu09    gorpv-h6mtj7    gorpv-h6n352    gorpv-h6mui9    gorpv-h6n4w7

Citations formats

Hiessl S, Schuldes J, Thurmer A, Halbsguth T, Broker D, Angelov A, Liebl W, Daniel R, Steinbuchel A (2012)
Involvement of two latex-clearing proteins during rubber degradation and insights into the subsequent degradation pathway revealed by the genome sequence of Gordonia polyisoprenivorans strain VH2
Applied Environmental Microbiology 78 :2874

Hiessl S, Schuldes J, Thurmer A, Halbsguth T, Broker D, Angelov A, Liebl W, Daniel R, Steinbuchel A (2012)
Applied Environmental Microbiology 78 :2874