Thurmer A

References (7)

Title : First Insights into the Genome Sequence of Pseudomonas oleovorans DSM 1045 - Poehlein_2017_Genome.Announc_5_
Author(s) : Poehlein A , Daniel R , Thurmer A , Bollinger A , Thies S , Katzke N , Jaeger KE
Ref : Genome Announc , 5 : , 2017
Abstract : The Gram-negative proteobacterium Pseudomonas oleovorans DSM 1045 is considered a promising source for enzymes of biotechnological interest, e.g., hydrolases and transaminases. Here, we present a draft sequence of its 4.86-Mb genome, enabling the identification of novel biocatalysts.
ESTHER : Poehlein_2017_Genome.Announc_5_
PubMedSearch : Poehlein_2017_Genome.Announc_5_
PubMedID: 28798180

Title : Unravelling the complete genome sequence of Advenella mimigardefordensis strain DPN7T and novel insights in the catabolism of the xenobiotic polythioester precursor 3,3'-dithiodipropionate - Wubbeler_2014_Microbiology_160_1401
Author(s) : Wubbeler JH , Hiessl S , Schuldes J , Thurmer A , Daniel R , Steinbuchel A
Ref : Microbiology , 160 :1401 , 2014
Abstract : Advenella mimigardefordensis strain DPN7(T) is a remarkable betaproteobacterium because of its extraordinary ability to use the synthetic disulfide 3,3'-dithiodipropionic acid (DTDP) as the sole carbon source and electron donor for aerobic growth. One application of DTDP is as a precursor substrate for biotechnically synthesized polythioesters (PTEs), which are interesting non-degradable biopolymers applicable for plastics materials. Metabolic engineering for optimization of PTE production requires an understanding of DTDP conversion. The genome of A. mimigardefordensis strain DPN7(T) was sequenced and annotated. The circular chromosome was found to be composed of 4,740,516 bp and 4112 predicted ORFs, whereas the circular plasmid consisted of 23,610 bp and 24 predicted ORFs. The genes participating in DTDP catabolism had been characterized in detail previously, but knowing the complete genome sequence and with support of Tn5: :mob-induced mutants, putatively involved transporter proteins and a transcriptional regulator were also identified. Most probably, DTDP is transported into the cell by a specific tripartite tricarboxylate transport system and is then cleaved by the disulfide reductase LpdA, sulfoxygenated by the 3-mercaptopropionate dioxygenase Mdo, activated by the CoA ligase SucCD and desulfinated by the acyl-CoA dehydrogenase-like desulfinase AcdA. Regulation of this pathway is presumably performed by a transcriptional regulator of the xenobiotic response element family. The excessive sulfate that is inevitably produced is secreted by the cells by a unique sulfate exporter of the CPA (cation : proton antiporter) superfamily.
ESTHER : Wubbeler_2014_Microbiology_160_1401
PubMedSearch : Wubbeler_2014_Microbiology_160_1401
PubMedID: 24739217
Gene_locus related to this paper: 9burk-w0pji8 , 9burk-w0p6d7

Title : Characterization and optimization of Bacillus subtilis ATCC 6051 as an expression host - Kabisch_2013_J.Biotechnol_163_97
Author(s) : Kabisch J , Thurmer A , Hubel T , Popper L , Daniel R , Schweder T
Ref : J Biotechnol , 163 :97 , 2013
Abstract : The genome sequence of Bacillus subtilis ATCC 6051 and its suitability as an expression host for recombinant protein production was determined. The comparison of this undomesticated wild type with the widely used laboratory strain B. subtilis 168 reveals a high degree of congruency between the two strains. Differences could only be detected on the level of point mutations or small insertions. B. subtilis ATCC 6051 shows none of the auxotrophies known for B. subtilis 168 and is able to produce polyketides. It exhibits better use of complex media and higher genomic stability through reduced natural competence. Consequently, B. subtilis ATCC 6051 was genetically modified to yield an optimized strain for the production of heterologously expressed proteins under control of an acetoin-inducible promoter.
ESTHER : Kabisch_2013_J.Biotechnol_163_97
PubMedSearch : Kabisch_2013_J.Biotechnol_163_97
PubMedID: 22789474
Gene_locus related to this paper: bacsu-pnbae , bacsu-YBAC

Title : Physiological homogeneity among the endosymbionts of Riftia pachyptila and Tevnia jerichonana revealed by proteogenomics - Gardebrecht_2012_ISME.J_6_766
Author(s) : Gardebrecht A , Markert S , Sievert SM , Felbeck H , Thurmer A , Albrecht D , Wollherr A , Kabisch J , Le Bris N , Lehmann R , Daniel R , Liesegang H , Hecker M , Schweder T
Ref : Isme J , 6 :766 , 2012
Abstract : The two closely related deep-sea tubeworms Riftia pachyptila and Tevnia jerichonana both rely exclusively on a single species of sulfide-oxidizing endosymbiotic bacteria for their nutrition. They do, however, thrive in markedly different geochemical conditions. A detailed proteogenomic comparison of the endosymbionts coupled with an in situ characterization of the geochemical environment was performed to investigate their roles and expression profiles in the two respective hosts. The metagenomes indicated that the endosymbionts are genotypically highly homogeneous. Gene sequences coding for enzymes of selected key metabolic functions were found to be 99.9% identical. On the proteomic level, the symbionts showed very consistent metabolic profiles, despite distinctly different geochemical conditions at the plume level of the respective hosts. Only a few minor variations were observed in the expression of symbiont enzymes involved in sulfur metabolism, carbon fixation and in the response to oxidative stress. Although these changes correspond to the prevailing environmental situation experienced by each host, our data strongly suggest that the two tubeworm species are able to effectively attenuate differences in habitat conditions, and thus to provide their symbionts with similar micro-environments.
ESTHER : Gardebrecht_2012_ISME.J_6_766
PubMedSearch : Gardebrecht_2012_ISME.J_6_766
PubMedID: 22011719
Gene_locus related to this paper: 9gamm-g2d8y1

Title : Involvement of two latex-clearing proteins during rubber degradation and insights into the subsequent degradation pathway revealed by the genome sequence of Gordonia polyisoprenivorans strain VH2 - Hiessl_2012_Appl.Environ.Microbiol_78_2874
Author(s) : Hiessl S , Schuldes J , Thurmer A , Halbsguth T , Broker D , Angelov A , Liebl W , Daniel R , Steinbuchel A
Ref : Applied Environmental Microbiology , 78 :2874 , 2012
Abstract : The increasing production of synthetic and natural poly(cis-1,4-isoprene) rubber leads to huge challenges in waste management. Only a few bacteria are known to degrade rubber, and little is known about the mechanism of microbial rubber degradation. The genome of Gordonia polyisoprenivorans strain VH2, which is one of the most effective rubber-degrading bacteria, was sequenced and annotated to elucidate the degradation pathway and other features of this actinomycete. The genome consists of a circular chromosome of 5,669,805 bp and a circular plasmid of 174,494 bp with average GC contents of 67.0% and 65.7%, respectively. It contains 5,110 putative protein-coding sequences, including many candidate genes responsible for rubber degradation and other biotechnically relevant pathways. Furthermore, we detected two homologues of a latex-clearing protein, which is supposed to be a key enzyme in rubber degradation. The deletion of these two genes for the first time revealed clear evidence that latex-clearing protein is essential for the microbial utilization of rubber. Based on the genome sequence, we predict a pathway for the microbial degradation of rubber which is supported by previous and current data on transposon mutagenesis, deletion mutants, applied comparative genomics, and literature search.
ESTHER : Hiessl_2012_Appl.Environ.Microbiol_78_2874
PubMedSearch : Hiessl_2012_Appl.Environ.Microbiol_78_2874
PubMedID: 22327575
Gene_locus related to this paper: gorpv-h6mtj1 , gorpv-h6mzw9 , gorpv-h6n0e3 , gorpv-h6n0g6 , gorpv-h6n0p7 , gorpv-h6mts4 , 9acto-h0rbp9 , 9acto-h0rc96 , gorpv-h6mu09 , gorpv-h6mtj7 , gorpv-h6n352 , gorpv-h6mui9 , gorpv-h6n4w7

Title : Genome sequence of Brevibacillus laterosporus LMG 15441, a pathogen of invertebrates - Djukic_2011_J.Bacteriol_193_5535
Author(s) : Djukic M , Poehlein A , Thurmer A , Daniel R
Ref : Journal of Bacteriology , 193 :5535 , 2011
Abstract : Here we announce the genome sequence of the bacterium Brevibacillus laterosporus LMG 15441, which is a pathogen of invertebrates. The genome consists of one chromosome and two circular plasmids. Sequence analysis revealed a large potential to produce polyketides, nonribosomal peptides, and toxins.
ESTHER : Djukic_2011_J.Bacteriol_193_5535
PubMedSearch : Djukic_2011_J.Bacteriol_193_5535
PubMedID: 21914864
Gene_locus related to this paper: brela-a0a075r7b1 , brela-a0a075r8m9 , brela-a0a075rfd2

Title : Comparative genomics and transcriptomics of Propionibacterium acnes - Brzuszkiewicz_2011_PLoS.One_6_e21581
Author(s) : Brzuszkiewicz E , Weiner J , Wollherr A , Thurmer A , Hupeden J , Lomholt HB , Kilian M , Gottschalk G , Daniel R , Mollenkopf HJ , Meyer TF , Bruggemann H
Ref : PLoS ONE , 6 :e21581 , 2011
Abstract : The anaerobic gram-positive bacterium Propionibacterium acnes is a human skin commensal that is occasionally associated with inflammatory diseases. Recent work has indicated that evolutionary distinct lineages of P. acnes play etiologic roles in disease while others are associated with maintenance of skin homeostasis. To shed light on the molecular basis for differential strain properties, we carried out genomic and transcriptomic analysis of distinct P. acnes strains. We sequenced the genome of the P. acnes strain 266, a type I-1a strain. Comparative genome analysis of strain 266 and four other P. acnes strains revealed that overall genome plasticity is relatively low; however, a number of island-like genomic regions, encoding a variety of putative virulence-associated and fitness traits differ between phylotypes, as judged from PCR analysis of a collection of P. acnes strains. Comparative transcriptome analysis of strains KPA171202 (type I-2) and 266 during exponential growth revealed inter-strain differences in gene expression of transport systems and metabolic pathways. In addition, transcript levels of genes encoding possible virulence factors such as dermatan-sulphate adhesin, polyunsaturated fatty acid isomerase, iron acquisition protein HtaA and lipase GehA were upregulated in strain 266. We investigated differential gene expression during exponential and stationary growth phases. Genes encoding components of the energy-conserving respiratory chain as well as secreted and virulence-associated factors were transcribed during the exponential phase, while the stationary growth phase was characterized by upregulation of genes involved in stress responses and amino acid metabolism. Our data highlight the genomic basis for strain diversity and identify, for the first time, the actively transcribed part of the genome, underlining the important role growth status plays in the inflammation-inducing activity of P. acnes. We argue that the disease-causing potential of different P. acnes strains is not only determined by the phylotype-specific genome content but also by variable gene expression.
ESTHER : Brzuszkiewicz_2011_PLoS.One_6_e21581
PubMedSearch : Brzuszkiewicz_2011_PLoS.One_6_e21581
PubMedID: 21738717
Gene_locus related to this paper: proac-q6a5t3 , proac-q6a5w7 , proac-q6a981