| Title : Structure-Guided Regulation in the Enantioselectivity of an Epoxide Hydrolase to Produce Enantiomeric Monosubstituted Epoxides and Vicinal Diols via Kinetic Resolution - Hu_2022_Org.Lett__ |
| Author(s) : Hu D , Hu BC , Hou XD , Zhang D , Lei YQ , Rao YJ , Wu MC |
| Ref : Org Lett , : , 2022 |
|
Abstract :
Structure-guided microtuning of an Aspergillus usamii epoxide hydrolase was executed. One mutant, A214C/A250I, displayed a 12.6-fold enhanced enantiomeric ratio (E = 202) toward rac-styrene oxide, achieving its nearly perfect kinetic resolution at 0.8 M in pure water or 1.6 M in n-hexanol/water. Several other beneficial mutants also displayed significantly improved E values, offering promising biocatalysts to access 19 structurally diverse chiral monosubstituted epoxides (97.1 - <= 99% ee(s)) and vicinal diols (56.2-98.0% ee(p)) with high yields. |
| PubMedSearch : Hu_2022_Org.Lett__ |
| PubMedID: 35229602 |
| Gene_locus related to this paper: aspng-q1ktb5 |
| Gene_locus | aspng-q1ktb5 |
| Family | Epoxide_hydrolase |
| Structure | 6IX2 |
Hu D, Hu BC, Hou XD, Zhang D, Lei YQ, Rao YJ, Wu MC (2022)
Structure-Guided Regulation in the Enantioselectivity of an Epoxide Hydrolase to Produce Enantiomeric Monosubstituted Epoxides and Vicinal Diols via Kinetic Resolution
Org Lett
:
Hu D, Hu BC, Hou XD, Zhang D, Lei YQ, Rao YJ, Wu MC (2022)
Org Lett
: