Title : Identification and Study of Biomarkers from Novichok-Inhibited Butyrylcholinesterase in Human Plasma - Jeong_2021_Molecules_26_3810 |
Author(s) : Jeong WH , Lee JY , Lim KC , Kim HS |
Ref : Molecules , 26 : , 2021 |
Abstract :
To identify biomarkers of ethyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A234)- or methyl (1-(diethylamino)ethylidene)phosphoramidofluoridate (A232)-inhibited butyrylcholinesterase (BChE), we investigated nonapeptide adducts containing the active site serine, which plays a key role in enzyme activity, using LC-MS/HRMS. Biomarkers were acquired as expected, and they exhibited a significant amount of fragment ions from the inhibiting agent itself, in contrast to the MS2 spectra of conventional nerve agents. These biomarkers had a higher abundance of [M+2H](2+) ions than [M+H](+) ions, making doubly charged ions more suitable for trace analysis. |
PubMedSearch : Jeong_2021_Molecules_26_3810 |
PubMedID: 34206601 |
Inhibitor | A-234-Nerve-agent A-232-Nerve-agent |
Jeong WH, Lee JY, Lim KC, Kim HS (2021)
Identification and Study of Biomarkers from Novichok-Inhibited Butyrylcholinesterase in Human Plasma
Molecules
26 :
Jeong WH, Lee JY, Lim KC, Kim HS (2021)
Molecules
26 :