Kim_2020_Biomolecules_10_

Reference

Title : Structural and Biophysical Analyses of Human N-Myc Downstream-Regulated Gene 3 (NDRG3) Protein - Kim_2020_Biomolecules_10_
Author(s) : Kim KR , Kim KA , Park JS , Jang JY , Choi Y , Lee HH , Lee DC , Park KC , Yeom YI , Kim HJ , Han BW
Ref : Biomolecules , 10 : , 2020
Abstract : The N-Myc downstream-regulated gene (NDRG) family belongs to the alpha/beta-hydrolase fold and is known to exert various physiologic functions in cell proliferation, dierentiation, and hypoxia-induced cancer metabolism. In particular, NDRG3 is closely related to proliferation and migration of prostate cancer cells, and recent studies reported its implication in lactate-triggered hypoxia responses or tumorigenesis. However, the underlying mechanism for the functions of NDRG3 remains unclear. Here, we report the crystal structure of human NDRG3 at 2.2 resolution, with six molecules in an asymmetric unit. While NDRG3 adopts the alpha/beta-hydrolase fold, complete substitution of the canonical catalytic triad residues to non-reactive residues and steric hindrance around the pseudo-active site seem to disable the alpha/beta-hydrolase activity. While NDRG3 shares a high similarity to NDRG2 in terms of amino acid sequence and structure, NDRG3 exhibited remarkable structural differences in a flexible loop corresponding to helix alpha6 of NDRG2 that is responsible for tumor suppression. Thus, this flexible loop region seems to play a distinct role in oncogenic progression induced by NDRG3. Collectively, our studies could provide structural and biophysical insights into the molecular characteristics of NDRG3.
ESTHER : Kim_2020_Biomolecules_10_
PubMedSearch : Kim_2020_Biomolecules_10_
PubMedID: 31935861
Gene_locus related to this paper: human-NDRG3

Related information

Gene_locus related to this paper: human-NDRG3

Citations formats

Kim KR, Kim KA, Park JS, Jang JY, Choi Y, Lee HH, Lee DC, Park KC, Yeom YI, Kim HJ, Han BW (2020)
Structural and Biophysical Analyses of Human N-Myc Downstream-Regulated Gene 3 (NDRG3) Protein
Biomolecules 10 :

Kim KR, Kim KA, Park JS, Jang JY, Choi Y, Lee HH, Lee DC, Park KC, Yeom YI, Kim HJ, Han BW (2020)
Biomolecules 10 :