Kruis_2017_Metab.Eng_41_92

Reference

Title : Ethyl acetate production by the elusive alcohol acetyltransferase from yeast - Kruis_2017_Metab.Eng_41_92
Author(s) : Kruis AJ , Levisson M , Mars AE , Van der Ploeg M , Garces Daza F , Ellena V , Kengen SWM , Van der Oost J , Weusthuis RA
Ref : Metab Eng , 41 :92 , 2017
Abstract : Ethyl acetate is an industrially relevant ester that is currently produced exclusively through unsustainable processes. Many yeasts are able to produce ethyl acetate, but the main responsible enzyme has remained elusive, hampering the engineering of novel production strains. Here we describe the discovery of a new enzyme (Eat1) from the yeast Wickerhamomyces anomalus that resulted in high ethyl acetate production when expressed in Saccharomyces cerevisiae and Escherichia coli. Purified Eat1 showed alcohol acetyltransferase activity with ethanol and acetyl-CoA. Homologs of eat1 are responsible for most ethyl acetate synthesis in known ethyl acetate-producing yeasts, including S. cerevisiae, and are only distantly related to known alcohol acetyltransferases. Eat1 is therefore proposed to compose a novel alcohol acetyltransferase family within the alpha/beta hydrolase superfamily. The discovery of this novel enzyme family is a crucial step towards the development of biobased ethyl acetate production and will also help in selecting improved S. cerevisiae brewing strains.
ESTHER : Kruis_2017_Metab.Eng_41_92
PubMedSearch : Kruis_2017_Metab.Eng_41_92
PubMedID: 28356220
Gene_locus related to this paper: wicao-a0a1e3p8s6 , wiccf-k0kpv8 , wicao-a0a1e3p8s8 , wiccf-k0ksn3 , cybfa-a0a061ayy2

Related information

Gene_locus related to this paper: wicao-a0a1e3p8s6 , wiccf-k0kpv8 , wicao-a0a1e3p8s8 , wiccf-k0ksn3 , cybfa-a0a061ayy2

Citations formats

Kruis AJ, Levisson M, Mars AE, Van der Ploeg M, Garces Daza F, Ellena V, Kengen SWM, Van der Oost J, Weusthuis RA (2017)
Ethyl acetate production by the elusive alcohol acetyltransferase from yeast
Metab Eng 41 :92

Kruis AJ, Levisson M, Mars AE, Van der Ploeg M, Garces Daza F, Ellena V, Kengen SWM, Van der Oost J, Weusthuis RA (2017)
Metab Eng 41 :92