Kwon_2010_Insect.Mol.Biol_19_195

Reference

Title : Extensive gene duplication of acetylcholinesterase associated with organophosphate resistance in the two-spotted spider mite - Kwon_2010_Insect.Mol.Biol_19_195
Author(s) : Kwon DH , Clark JM , Lee SH
Ref : Insect Molecular Biology , 19 :195 , 2010
Abstract :

Monocrotophos-resistant two-spotted spider mites (TSSMs), Tetranychus urticae, are known to possess three mutations on the acetylcholinesterase (AChE) gene (Tuace) that are involved in target site insensitivity. Cross-strain comparison of three strains (highly resistant AD, moderately resistant PyriF and susceptible UD strains) revealed that resistant strains have relatively more Tuace copies than the UD strain and that the levels of transcript were directly proportional to copy numbers. AChEs from the AD and PyriF strains had similar V(max) values to those of AChE from the UD strain but increased K(m) and reduced k(cat) constants, suggesting that the mutated, resistant form of AChE may carry a fitness cost. Relative copy numbers of Tuace in field populations varied from 2.4 to 6.1, correlating well with their levels of resistance (r(2)= 0.895). These results are suggestive of the involvement of Tuace gene duplication in resistance. Thus, monocrotophos resistance in TSSMs appears to have evolved through a combination of mutation accumulation and extensive gene duplication.

PubMedSearch : Kwon_2010_Insect.Mol.Biol_19_195
PubMedID: 20002213
Gene_locus related to this paper: tetur-ACHE

Related information

Mutation G119S_tetur-ACHE    F331W_tetur-ACHE    A280T_tetur-ACHE
Gene_locus tetur-ACHE

Citations formats

Kwon DH, Clark JM, Lee SH (2010)
Extensive gene duplication of acetylcholinesterase associated with organophosphate resistance in the two-spotted spider mite
Insect Molecular Biology 19 :195

Kwon DH, Clark JM, Lee SH (2010)
Insect Molecular Biology 19 :195