| Title : Didepside Formation by the Nonreducing Polyketide Synthase Preu6 of Preussia isomera Requires Interaction of Starter Acyl Transferase and Thioesterase Domains - Liu_2023_Angew.Chem.Int.Ed.Engl_62_e202214379 |
| Author(s) : Liu Q , Zhang D , Gao S , Cai X , Yao M , Xu Y , Gong Y , Zheng K , Mao Y , Yang L , Yang D , Molnar I , Yang X |
| Ref : Angew Chem Int Ed Engl , 62 :e202214379 , 2023 |
| Abstract : Orsellinic acid (OA) derivatives are produced by filamentous fungi using nonreducing polyketide synthases (nrPKSs). The chain-releasing thioesterase (TE) domains of such nrPKSs were proposed to also catalyze dimerization to yield didepsides, such as lecanoric acid. Here, we use combinatorial domain exchanges, domain dissections and reconstitutions to reveal that the TE domain of the lecanoric acid synthase Preu6 of Preussia isomera must collaborate with the starter acyl transferase (SAT) domain from the same nrPKS. We show that artificial SAT-TE fusion proteins are highly effective catalysts and reprogram the ketide homologation chassis to form didepsides. We also demonstrate that dissected SAT and TE domains of Preu6 physically interact, and SAT and TE domains of OA-synthesizing nrPKSs may co-evolve. Our work highlights an unexpected domain-domain interaction in nrPKSs that must be considered for the combinatorial biosynthesis of unnatural didepsides, depsidones, and diphenyl ethers. |
| ESTHER : Liu_2023_Angew.Chem.Int.Ed.Engl_62_e202214379 |
| PubMedSearch : Liu_2023_Angew.Chem.Int.Ed.Engl_62_e202214379 |
| PubMedID: 36484777 |
| Gene_locus related to this paper: preis-preu6 |
| Gene_locus related to this paper: preis-preu6 |
Liu Q, Zhang D, Gao S, Cai X, Yao M, Xu Y, Gong Y, Zheng K, Mao Y, Yang L, Yang D, Molnar I, Yang X (2023)
Didepside Formation by the Nonreducing Polyketide Synthase Preu6 of Preussia isomera Requires Interaction of Starter Acyl Transferase and Thioesterase Domains
Angew Chem Int Ed Engl
62 :e202214379
Liu Q, Zhang D, Gao S, Cai X, Yao M, Xu Y, Gong Y, Zheng K, Mao Y, Yang L, Yang D, Molnar I, Yang X (2023)
Angew Chem Int Ed Engl
62 :e202214379