Lopez-Soler_2011_Pest.Manag.Sci_67_1549

Reference

Title : Esterase inhibition by synergists in the western flower thrips Frankliniella occidentalis - Lopez-Soler_2011_Pest.Manag.Sci_67_1549
Author(s) : Lopez-Soler N , Cervera A , Quinto V , Abellan J , Bielza P , Martinez-Pardo R , Garcera MD
Ref : Pest Manag Sci , 67 :1549 , 2011
Abstract : BACKGROUND: Western flower thrips (WFT), Frankliniella occidentalis (Pergande), is among the most important crop pests in the south-eastern region of Spain. Its increasing resistance to insecticides constitutes a serious problem, and understanding the mechanisms involved is therefore of great interest. Use of synergists to inhibit the enzymes involved in insecticide detoxification is widely used to determine their responsibility for insecticide resistance. However, they do not always act as intended or expected, and caution must be exercised when interpreting synergist results. RESULTS: Laboratory-selected strains of WFT were used to analyse the effects of the synergists piperonyl butoxide (PBO), S,S,S-tributyl phosphorotrithioate (DEF) and methiocarb on total esterase activity. Significant differences were found, indicating esterase activity inhibition by DEF, a lower effect for methiocarb and a small inhibition of the activity by PBO. Esterase isoenzyme inhibition by these compounds showed a similar result; this assay revealed an extreme sensitivity of Triplet A (resistance-associated esterases) to DEF. In an in vivo assay carried out with these compounds at different incubation times, only DEF caused posterior in vitro esterase activity inhibition, with a maximum effect 1 h after treatment. CONCLUSION: In this work, only DEF shows true synergistic inhibition of WFT esterases.
ESTHER : Lopez-Soler_2011_Pest.Manag.Sci_67_1549
PubMedSearch : Lopez-Soler_2011_Pest.Manag.Sci_67_1549
PubMedID: 21656898

Related information

Citations formats

Lopez-Soler N, Cervera A, Quinto V, Abellan J, Bielza P, Martinez-Pardo R, Garcera MD (2011)
Esterase inhibition by synergists in the western flower thrips Frankliniella occidentalis
Pest Manag Sci 67 :1549

Lopez-Soler N, Cervera A, Quinto V, Abellan J, Bielza P, Martinez-Pardo R, Garcera MD (2011)
Pest Manag Sci 67 :1549