Bielza P

References (15)

Title : Global patterns in genomic diversity underpinning the evolution of insecticide resistance in the aphid crop pest Myzus persicae - Singh_2021_Commun.Biol_4_847
Author(s) : Singh KS , Cordeiro EMG , Troczka BJ , Pym A , Mackisack J , Mathers TC , Duarte A , Legeai F , Robin S , Bielza P , Burrack HJ , Charaabi K , Denholm I , Figueroa CC , ffrench-Constant RH , Jander G , Margaritopoulos JT , Mazzoni E , Nauen R , Ramirez CC , Ren G , Stepanyan I , Umina PA , Voronova NV , Vontas J , Williamson MS , Wilson ACC , Xi-Wu G , Youn YN , Zimmer CT , Simon JC , Hayward A , Bass C
Ref : Commun Biol , 4 :847 , 2021
Abstract : The aphid Myzus persicae is a destructive agricultural pest that displays an exceptional ability to develop resistance to both natural and synthetic insecticides. To investigate the evolution of resistance in this species we generated a chromosome-scale genome assembly and living panel of >110 fully sequenced globally sampled clonal lines. Our analyses reveal a remarkable diversity of resistance mutations segregating in global populations of M. persicae. We show that the emergence and spread of these mechanisms is influenced by host-plant associations, uncovering the widespread co-option of a host-plant adaptation that also offers resistance against synthetic insecticides. We identify both the repeated evolution of independent resistance mutations at the same locus, and multiple instances of the evolution of novel resistance mechanisms against key insecticides. Our findings provide fundamental insights into the genomic responses of global insect populations to strong selective forces, and hold practical relevance for the control of pests and parasites.
ESTHER : Singh_2021_Commun.Biol_4_847
PubMedSearch : Singh_2021_Commun.Biol_4_847
PubMedID: 34234279

Title : Mutation in the ace-1 gene of the tomato leaf miner (Tuta absoluta) associated with organophosphates resistance - Haddi_2017_J.Appl.Entomol_141_612
Author(s) : Haddi K , Berger M , Bielza P , Rapisarda C , Williamson MS , Moores GD , Bass C
Ref : J.Appl_Entomol , 141 :612 , 2017
Abstract : The tomato leaf miner, Tuta absoluta (Lepidoptera: Gelechiidae), is a major invasive pest that has spread throughout many countries in the Mediterranean basin and parts of Asia over the last decade. The control of T. absoluta has relied heavily on the use of chemical insecticides, a strategy that has led to the evolution of resistance. In this study, biological and molecular methods were used to determine the susceptibility of five strains of T. absoluta to the organophosphate chlorpyrifos and to investigate the molecular mechanisms underlying resistance to this class of insecticides. High levels of resistance to chlorpyrifos were observed in all five strains tested. Cloning and sequencing of the gene encoding the organophosphate target site, ace-1, of T. absoluta revealed the presence of an alanine to serine substitution at a position that has been previously linked with organophosphate resistance across a range of different insect and mite species. The presence of this mutation at high frequency in T. absoluta populations originating from various countries further supports the suggestion that the rapid expansion of this species is, in part, mediated by the resistance of this pest to chemical insecticides.
ESTHER : Haddi_2017_J.Appl.Entomol_141_612
PubMedSearch : Haddi_2017_J.Appl.Entomol_141_612
Gene_locus related to this paper: tutab-ACHE1

Title : Ryanodine receptor point mutations confer diamide insecticide resistance in tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae) - Roditakis_2017_Insect.Biochem.Mol.Biol_80_11
Author(s) : Roditakis E , Steinbach D , Moritz G , Vasakis E , Stavrakaki M , Ilias A , Garcia-Vidal L , Martinez-Aguirre MD , Bielza P , Morou E , Silva JE , Silva WM , Siqueira Eta A , Iqbal S , Troczka BJ , Williamson MS , Bass C , Tsagkarakou A , Vontas J , Nauen R
Ref : Insect Biochemistry & Molecular Biology , 80 :11 , 2017
Abstract : Insect ryanodine receptors (RyR) are the molecular target-site for the recently introduced diamide insecticides. Diamides are particularly active on Lepidoptera pests, including tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae). High levels of diamide resistance were recently described in some European populations of T. absoluta, however, the mechanisms of resistance remained unknown. In this study the molecular basis of diamide resistance was investigated in a diamide resistant strain from Italy (IT-GELA-SD4), and additional resistant field populations collected in Greece, Spain and Brazil. The genetics of resistance was investigated by reciprocally crossing strain IT-GELA-SD4 with a susceptible strain and revealed an autosomal incompletely recessive mode of inheritance. To investigate the possible role of target-site mutations as known from diamondback moth (Plutella xylostella), we sequenced respective domains of the RyR gene of T. absoluta. Genotyping of individuals of IT-GELA-SD4 and field-collected strains showing different levels of diamide resistance revealed the presence of G4903E and I4746M RyR target-site mutations. These amino acid substitutions correspond to those recently described for diamide resistant diamondback moth, i.e. G4946E and I4790M. We also detected two novel mutations, G4903V and I4746T, in some of the resistant T. absoluta strains. Radioligand binding studies with thoracic membrane preparations of the IT-GELA-SD4 strain provided functional evidence that these mutations alter the affinity of the RyR to diamides. In combination with previous work on P. xylostella our study highlights the importance of position G4903 (G4946 in P. xylostella) of the insect RyR in defining sensitivity to diamides. The discovery of diamide resistance mutations in T. absoluta populations of diverse geographic origin has serious implications for the efficacy of diamides under applied conditions. The implementation of appropriate resistance management strategies is strongly advised to delay the further spread of resistance.
ESTHER : Roditakis_2017_Insect.Biochem.Mol.Biol_80_11
PubMedSearch : Roditakis_2017_Insect.Biochem.Mol.Biol_80_11
PubMedID: 27845250

Title : Insecticide resistance mediated by an exon skipping event - Berger_2016_Mol.Ecol_25_5692
Author(s) : Berger M , Puinean AM , Randall E , Zimmer CT , Silva WM , Bielza P , Field LM , Hughes D , Mellor I , Hassani-Pak K , Siqueira HA , Williamson MS , Bass C
Ref : Mol Ecol , 25 :5692 , 2016
Abstract : Many genes increase coding capacity by alternate exon usage. The gene encoding the insect nicotinic acetylcholine receptor (nAChR) alpha6 subunit, target of the bio-insecticide spinosad, is one example of this and expands protein diversity via alternative splicing of mutually exclusive exons. Here, we show that spinosad resistance in the tomato leaf miner, Tuta absoluta is associated with aberrant regulation of splicing of Taalpha6 resulting in a novel form of insecticide resistance mediated by exon skipping. Sequencing of the alpha6 subunit cDNA from spinosad selected and unselected strains of T. absoluta revealed all Taalpha6 transcripts of the selected strain were devoid of exon 3, with comparison of genomic DNA and mRNA revealing this is a result of exon skipping. Exon skipping cosegregated with spinosad resistance in survival bioassays, and functional characterization of this alteration using modified human nAChR alpha7, a model of insect alpha6, demonstrated that exon 3 is essential for receptor function and hence spinosad sensitivity. DNA and RNA sequencing analyses suggested that exon skipping did not result from genetic alterations in intronic or exonic cis-regulatory elements, but rather was associated with a single epigenetic modification downstream of exon 3a, and quantitative changes in the expression of trans-acting proteins that have known roles in the regulation of alternative splicing. Our results demonstrate that the intrinsic capacity of the alpha6 gene to generate transcript diversity via alternative splicing can be readily exploited during the evolution of resistance and identifies exon skipping as a molecular alteration conferring insecticide resistance.
ESTHER : Berger_2016_Mol.Ecol_25_5692
PubMedSearch : Berger_2016_Mol.Ecol_25_5692
PubMedID: 27748560

Title : A nicotinic acetylcholine receptor transmembrane point mutation (G275E) associated with resistance to spinosad in Frankliniella occidentalis - Puinean_2013_J.Neurochem_124_590
Author(s) : Puinean AM , Lansdell SJ , Collins T , Bielza P , Millar NS
Ref : Journal of Neurochemistry , 124 :590 , 2013
Abstract : High levels of resistance to spinosad, a macrocyclic lactone insecticide, have been reported previously in western flower thrips, Frankliniella occidentalis, an economically important insect pest of vegetables, fruit and ornamental crops. We have cloned the nicotinic acetylcholine receptor (nAChR) alpha6 subunit from F. occidentalis (Foalpha6) and compared the nucleotide sequence of Foalpha6 from susceptible and spinosad-resistant insect populations (MLFOM and R1S respectively). A single nucleotide change has been identified in Foalpha6, resulting in the replacement of a glycine (G) residue in susceptible insects with a glutamic acid (E) in resistant insects. The resistance-associated mutation (G275E) is predicted to lie at the top of the third alpha-helical transmembrane domain of Foalpha6. Although there is no direct evidence identifying the location of the spinosad binding site, the analogous amino acid in the C. elegans glutamate-gated chloride channel lies in close proximity (4.4 A) to the known binding site of ivermectin, another macrocyclic lactone pesticide. The functional consequences of the resistance-associated mutation have been examined in the human nAChR alpha7 subunit. Introduction of an analogous (A272E) mutation in alpha7 abolishes the modulatory effects of spinosad whilst having no significant effect upon activation by acetylcholine, consistent with spinosad having an allosteric mechanism of action.
ESTHER : Puinean_2013_J.Neurochem_124_590
PubMedSearch : Puinean_2013_J.Neurochem_124_590
PubMedID: 23016960

Title : Determination of baseline susceptibility of European populations of Tuta absoluta (Meyrick) to indoxacarb and chlorantraniliprole using a novel dip bioassay method - Roditakis_2013_Pest.Manag.Sci_69_217
Author(s) : Roditakis E , Skarmoutsou C , Staurakaki M , Martinez-Aguirre Mdel R , Garcia-Vidal L , Bielza P , Haddi K , Rapisarda C , Rison JL , Bassi A , Teixeira LA
Ref : Pest Manag Sci , 69 :217 , 2013
Abstract : BACKGROUND: Tuta absoluta(Meyrick) is one of the most serious pests of tomato recently introduced in the Mediterranean region. A novel bioassay method designed for the accurate determination of insecticide toxicity on T. absoluta (IRAC method No. 022) was validated by three different laboratories [Greece (NAGREF), Italy (UC) and Spain (UPCT)] on European populations.
RESULTS: The insecticides indoxacarb and chlorantraniliprole were used as reference products. The IRAC leaf dip method is easy to perform, producing repeatable, homogeneous responses. LC(50) values for indoxacarb ranged between 1.8 and 17.9 mg L(-1) (NAGREF), 0.93 and 10.8 mg L(-1) (UC) and 0.20 and 0.70 mg L(-1) (UPCT), resulting in a tenfold, 12-fold and fourfold difference between the least and most susceptible populations at each laboratory respectively. For chlorantraniliprole, LC(50) values ranged between 0.10 and 0.56 mg L(-1) (NAGREF), 0.23 and 1.34 mg L(-1) (UC) and 0.04 and 0.24 mg L(-1) (UPCT), resulting in a sixfold difference in all three cases. Overall, UPCT reported lower mean LC(50) to indoxacarb, while UC reported higher LC(50) to chlorantraniliprole.
CONCLUSIONS: The new bioassay is reliable, providing a useful tool in the design of IRM strategies. Within each country/lab, the variability observed in the results for both indoxacarb and chlorantraniliprole can be attributed to natural variation. Future research is necessary to determine the extent to which it is possible to compare results among laboratories.
ESTHER : Roditakis_2013_Pest.Manag.Sci_69_217
PubMedSearch : Roditakis_2013_Pest.Manag.Sci_69_217
PubMedID: 23034903

Title : Identification of mutations associated with pyrethroid resistance in the voltage-gated sodium channel of the tomato leaf miner (Tuta absoluta) - Haddi_2012_Insect.Biochem.Mol.Biol_42_506
Author(s) : Haddi K , Berger M , Bielza P , Cifuentes D , Field LM , Gorman K , Rapisarda C , Williamson MS , Bass C
Ref : Insect Biochemistry & Molecular Biology , 42 :506 , 2012
Abstract : The tomato leaf miner, Tuta absoluta (Lepidoptera) is a significant pest of tomatoes that has undergone a rapid expansion in its range during the past six years and is now present across Europe, North Africa and parts of Asia. One of the main means of controlling this pest is through the use of chemical insecticides. In the current study insecticide bioassays were used to determine the susceptibility of five T. absoluta strains established from field collections from Europe and Brazil to pyrethroids. High levels of resistance to lambda cyhalothrin and tau fluvalinate were observed in all five strains tested. To investigate whether pyrethroid resistance was mediated by mutation of the para-type sodium channel in T. absoluta the IIS4-IIS6 region of the para gene, which contains many of the mutation sites previously shown to confer knock down (kdr)-type resistance to pyrethroids across a range of different arthropod species, was cloned and sequenced. This revealed that three kdr/super-kdr-type mutations (M918T, T929I and L1014F), were present at high frequencies within all five resistant strains at known resistance 'hot-spots'. This is the first description of these mutations together in any insect population. High-throughput DNA-based diagnostic assays were developed and used to assess the prevalence of these mutations in 27 field strains from 12 countries. Overall mutant allele frequencies were high (L1014F 0.98, M918T 0.35, T929I 0.60) and remarkably no individual was observed that did not carry kdr in combination with either M918T or T929I. The presence of these mutations at high frequency in T. absoluta populations across much of its range suggests pyrethroids are likely to be ineffective for control and supports the idea that the rapid expansion of this species over the last six years may be in part mediated by the resistance of this pest to chemical insecticides.
ESTHER : Haddi_2012_Insect.Biochem.Mol.Biol_42_506
PubMedSearch : Haddi_2012_Insect.Biochem.Mol.Biol_42_506
PubMedID: 22504519

Title : Esterase inhibition by synergists in the western flower thrips Frankliniella occidentalis - Lopez-Soler_2011_Pest.Manag.Sci_67_1549
Author(s) : Lopez-Soler N , Cervera A , Quinto V , Abellan J , Bielza P , Martinez-Pardo R , Garcera MD
Ref : Pest Manag Sci , 67 :1549 , 2011
Abstract : BACKGROUND: Western flower thrips (WFT), Frankliniella occidentalis (Pergande), is among the most important crop pests in the south-eastern region of Spain. Its increasing resistance to insecticides constitutes a serious problem, and understanding the mechanisms involved is therefore of great interest. Use of synergists to inhibit the enzymes involved in insecticide detoxification is widely used to determine their responsibility for insecticide resistance. However, they do not always act as intended or expected, and caution must be exercised when interpreting synergist results. RESULTS: Laboratory-selected strains of WFT were used to analyse the effects of the synergists piperonyl butoxide (PBO), S,S,S-tributyl phosphorotrithioate (DEF) and methiocarb on total esterase activity. Significant differences were found, indicating esterase activity inhibition by DEF, a lower effect for methiocarb and a small inhibition of the activity by PBO. Esterase isoenzyme inhibition by these compounds showed a similar result; this assay revealed an extreme sensitivity of Triplet A (resistance-associated esterases) to DEF. In an in vivo assay carried out with these compounds at different incubation times, only DEF caused posterior in vitro esterase activity inhibition, with a maximum effect 1 h after treatment. CONCLUSION: In this work, only DEF shows true synergistic inhibition of WFT esterases.
ESTHER : Lopez-Soler_2011_Pest.Manag.Sci_67_1549
PubMedSearch : Lopez-Soler_2011_Pest.Manag.Sci_67_1549
PubMedID: 21656898

Title : Life-stage variation in insecticide resistance of the western flower thrips (Thysanoptera: Thripidae) - Contreras_2010_J.Econ.Entomol_103_2164
Author(s) : Contreras J , Espinosa PJ , Quinto V , Abellan J , Gravalos C , Fernandez E , Bielza P
Ref : J Econ Entomol , 103 :2164 , 2010
Abstract : The life-stage variations in insecticide resistance of western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), to selective insecticides (acrinathrin, formetanate, and methiocarb) were studied using resistant laboratory strains. In each strain, the second-instar larva was less susceptible to the insecticides tested than the adults. The lower the resistance level of the adults, the higher the difference between larva and adult susceptibility: 32-fold to methiocarb, 15.4-fold to formetanate, and 180-fold to acrinathrin in the reference strain. In laboratory-selected resistant strains, these differences were much lower: 5.8-fold to methiocarb, 4.8-fold to formetanate, and 2.0-fold to acrinathrin. In selected strains, higher resistance levels for each insecticide were found, both for larvae and adults, compared with the reference strain. These results show that after insecticide resistance selection in adults, the resistance is carried over to the larvae, but at lower levels.
ESTHER : Contreras_2010_J.Econ.Entomol_103_2164
PubMedSearch : Contreras_2010_J.Econ.Entomol_103_2164
PubMedID: 21309240

Title : Insecticide resistance management strategies against the western flower thrips, Frankliniella occidentalis - Bielza_2008_Pest.Manag.Sci_64_1131
Author(s) : Bielza P
Ref : Pest Manag Sci , 64 :1131 , 2008
Abstract : Western flower thrips (WFT), Frankliniella occidentalis (Pergande), is an economically important pest of a wide range of crops grown throughout the world. Insecticide resistance has been documented in many populations of WFT. Biological and behavioural characteristics and pest management practices that promote insecticide resistance are discussed. In addition, an overview is provided of the development of insecticide resistance in F. occidentalis populations and the resistance mechanisms involved. Owing to widespread resistance to most conventional insecticides, a new approach to insecticide resistance management (IRM) of F. occidentalis is needed. The IRM strategy proposed consists of two parts. Firstly, a general strategy to minimise the use of insecticides in order to reduce selection pressure. Secondly, a strategy designed to avoid selection of resistance mechanisms, considering cross-resistance patterns and resistance mechanisms.
ESTHER : Bielza_2008_Pest.Manag.Sci_64_1131
PubMedSearch : Bielza_2008_Pest.Manag.Sci_64_1131
PubMedID: 18561150

Title : Age-specific expression of resistance to a neonicotinoid insecticide in the whitefly Bemisia tabaci - Nauen_2008_Pest.Manag.Sci_64_1106
Author(s) : Nauen R , Bielza P , Denholm I , Gorman K
Ref : Pest Manag Sci , 64 :1106 , 2008
Abstract : Neonicotinoid insecticides retain a crucial role within many chemical and integrated control strategies for the tobacco whitefly, Bemisia tabaci Gennadius, in spite of the establishment of potent and widespread resistance in many areas. Metabolic resistance mechanisms mediated by overexpression of P450-dependent monooxygenases have been implicated in neonicotinoid resistance in the two most prevalent B. tabaci biotypes. Further characterisation of resistance to the neonicotinoid imidacloprid in populations of both these B- and Q-types is reported.Expression of resistance to imidacloprid was age specific in B- and Q-type strains of B. tabaci. The highest observed resistance ratio at LC(50) expressed in prepupal nymphs was 13, compared with at least 580 in their adult counterparts. For all strains, resistance expressed in immatures was not sufficiently potent to compromise recommended imidacloprid application rates.Targeting neonicotinoids towards immature life stages of B. tabaci may circumvent the protection conferred by current mechanisms of resistance, simultaneously reducing the selection pressures imposed. However, such tactics may enhance the expression of existing resistance mechanisms in immatures, or promote the establishment of novel ones expressed in all life stages.
ESTHER : Nauen_2008_Pest.Manag.Sci_64_1106
PubMedSearch : Nauen_2008_Pest.Manag.Sci_64_1106
PubMedID: 18798168

Title : Impact of production system on development of insecticide resistance in Frankliniella occidentalis (Thysanoptera: Thripidae) - Bielza_2008_J.Econ.Entomol_101_1685
Author(s) : Bielza P , Quinto V , Gravalos C , Fernandez E , Abellan J
Ref : J Econ Entomol , 101 :1685 , 2008
Abstract : The western flower thrips, Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), has become one of the most difficult insects to control in the intensive agriculture of southeastern Spain. However, resistance problems are quite different in two neighboring areas, Murcia and Almeria, with distinct production systems. Thirty-six field populations of western flower thrips from sweet pepper crops were collected in two different dates in Murcia and Almeria in 2005 and 2006. Western flower thrips populations collected were exposed to a diagnostic concentration of spinosad, methiocarb, acrinathrin, and formetanate. The results allowed the recognition of higher levels of resistance in Almeria compared with Murcia throughout the growing season. The mortality at the diagnostic concentration for spinosad (120 ppm) in western flower thrips populations ranged from 34 to 81% in Almeria, and from 73 to 100% in Murcia. The mortalities at the diagnostic concentration to acrinathrin (800 ppm) and formetanate (8000 ppm) were 17-31% in Almeria and 77-100% in Murcia, and 14-41% in Almeria and 48-99% in Murcia, respectively, indicating large geographic variations. Toxicity of methiocarb was higher for western flower thrips populations from both areas. However, mortality at the diagnostic concentration of methiocarb (2000 ppm) varied from 56 to 90% in Almeria, and it was from 94 to 100% in Murcia. The impact of production systems and agricultural practices of each area on the development and stability of insecticide resistance is discussed.
ESTHER : Bielza_2008_J.Econ.Entomol_101_1685
PubMedSearch : Bielza_2008_J.Econ.Entomol_101_1685
PubMedID: 18950052

Title : Relationship between esterase activity and acrinathrin and methiocarb resistance in field populations of western flower thrips, Frankliniella occidentalis - Mayma_2006_Pest.Manag.Sci_62_1129
Author(s) : Mayma AC , Cervera A , Dolores Garcera M , Bielza P , Martinez-Pardo R
Ref : Pest Manag Sci , 62 :1129 , 2006
Abstract : The western flower thrips Frankliniella occidentalis (Pergande) is a very significant pest of a number of different agricultural crops in the south-east of Spain. The importance of thrips as a pest is not due mainly to the direct damage inflicted on the plant, but to the loss in commercial value which occurs as a consequence of the development of dark spots caused by the tomato spotted wilt virus (TSWV) which they transmit. The economic threshold is therefore almost zero, which enhances the problems of resistance management. The present work is part of a global project that attempts to evaluate the status of insecticide resistance in field populations of thrips obtained from several agricultural crops. We have studied, in either individual or pooled insects, some enzyme systems classically related to detoxification of insecticides: esterase and glutathione-S-transferase (GST). The activity of these enzymes from laboratory populations selected with various classes of insecticides has also been measured using several appropriate substrates. An increase in GST mean activity was found in two field-collected strains. Differences in frequency distributions of esterase and GST activities were found for both field-collected strains and for a laboratory strain selected with acrinathrin. These activities were compared with those of a wild-type reference strain.
ESTHER : Mayma_2006_Pest.Manag.Sci_62_1129
PubMedSearch : Mayma_2006_Pest.Manag.Sci_62_1129

Title : Metabolic mechanisms of insecticide resistance in the western flower thrips, Frankliniella occidentalis (Pergande) - Espinosa_2005_Pest.Manag.Sci_61_1009
Author(s) : Espinosa PJ , Contreras J , Quinto V , Gravalos C , Fernandez E , Bielza P
Ref : Pest Manag Sci , 61 :1009 , 2005
Abstract : The interactions between six insecticides (methiocarb, formetanate, acrinathrin, deltamethrin, methamidophos and endosulfan) and three potential synergists (piperonyl butoxide (PBO), S,S,S-tributyl phosphorotrithioate (DEF) and diethyl maleate (DEM)) were studied by topical exposure in strains selected for resistance to each insecticide, and in a susceptible strain of Frankliniella occidentalis (Pergande). In the susceptible strain PBO produced appreciable synergism only of formetanate, methiocarb and methamidophos. Except for endosulfan, PBO synergized all the insecticides to varying degrees in the resistant strains. A very high level of synergism by PBO was found with acrinathrin, which reduced the resistance level from 3344- to 36-fold. PBO slightly synergized the carbamates formetanate (4.6-fold) and methiocarb (3.3-fold). PBO also produced a high synergism of deltamethrin (12.5-fold) and methamidophos (14.3-fold) and completely restored susceptibility to both insecticides. DEF did not produce synergism with any insecticide in the resistant strains and DEM was slightly synergistic to endosulfan (3-fold). These studies indicate that an enhanced detoxification, mediated by cytochrome P-450 monooxygenases, is the major mechanism imparting resistance to different insecticides in F occidentalis. Implications of different mechanisms in insecticide resistance in F occidentalis are discussed.
ESTHER : Espinosa_2005_Pest.Manag.Sci_61_1009
PubMedSearch : Espinosa_2005_Pest.Manag.Sci_61_1009
PubMedID: 15912569

Title : Insecticide resistance in field populations of Frankliniella occidentalis (Pergande) in Murcia (south-east Spain) - Espinosa_2002_Pest.Manag.Sci_58_967
Author(s) : Espinosa PJ , Bielza P , Contreras J , Lacasa A
Ref : Pest Manag Sci , 58 :967 , 2002
Abstract : Thirty-nine field populations of Frankliniella occidentalis (Pergande) were collected from different crops (sweet pepper, tomato, lettuce, artichoke, melon, cucumber, carnation, broad bean, peach and plum) in Murcia (south-east Spain). All populations were reared separately in the laboratory to obtain enough individuals for bioassays. Female thrips were bioassayed, using a standard topical application method, against methiocarb, methamidophos, acrinathrin, endosulfan, deltamethrin and formetanate. Methiocarb was the only insecticide that showed a high efficacy against F occidentalis at field dose rates. Acrinathrin and methamidophos were moderately effective, while endosulfan and deltamethrin were ineffective. Only moderate levels of resistance (Resistance Ratios at LC50 of 10-30) were detected for the selective insecticides methiocarb, formetanate and acrinathrin used against F occidentalis in crops where these insecticides are used intensively. This generalized and low level of resistance to these insecticides, coupled with a lack of efficacy for the three broad-spectrum insecticides, was observed even in intensively managed vegetable crops. Implementation of IPM strategies in Murcia has contributed to more successful insecticide anti-resistance management..
ESTHER : Espinosa_2002_Pest.Manag.Sci_58_967
PubMedSearch : Espinosa_2002_Pest.Manag.Sci_58_967
PubMedID: 12233190