| Title : Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group - Meyerdierks_2010_Environ.Microbiol_12_422 |
| Author(s) : Meyerdierks A , Kube M , Kostadinov I , Teeling H , Glockner FO , Reinhardt R , Amann R |
| Ref : Environ Microbiol , 12 :422 , 2010 |
| Abstract : Microbial consortia mediating the anaerobic oxidation of methane with sulfate are composed of methanotrophic Archaea (ANME) and Bacteria related to sulfate-reducing Deltaproteobacteria. Cultured representatives are not available for any of the three ANME clades. Therefore, a metagenomic approach was applied to assess the genetic potential of ANME-1 archaea. In total, 3.4 Mbp sequence information was generated based on metagenomic fosmid libraries constructed directly from a methanotrophic microbial mat in the Black Sea. These sequence data represent, in 30 contigs, about 82-90% of a composite ANME-1 genome. The dataset supports the hypothesis of a reversal of the methanogenesis pathway. Indications for an assimilatory, but not for a dissimilatory sulfate reduction pathway in ANME-1, were found. Draft genome and expression analyses are consistent with acetate and formate as putative electron shuttles. Moreover, the dataset points towards downstream electron-accepting redox components different from the ones known from methanogenic archaea. Whereas catalytic subunits of [NiFe]-hydrogenases are lacking in the dataset, genes for an [FeFe]-hydrogenase homologue were identified, not yet described to be present in methanogenic archaea. Clustered genes annotated as secreted multiheme c-type cytochromes were identified, which have not yet been correlated with methanogenesis-related steps. The genes were shown to be expressed, suggesting direct electron transfer as an additional possible mode to shuttle electrons from ANME-1 to the bacterial sulfate-reducing partner. |
| ESTHER : Meyerdierks_2010_Environ.Microbiol_12_422 |
| PubMedSearch : Meyerdierks_2010_Environ.Microbiol_12_422 |
| PubMedID: 19878267 |
| Gene_locus related to this paper: 9arch-d1j9b3 , 9arch-d1ja37 , 9arch-d1jaz1 , 9arch-q64ad0 |
| Gene_locus related to this paper: 9arch-d1j9b3 , 9arch-d1ja37 , 9arch-d1jaz1 , 9arch-q64ad0 |
Meyerdierks A, Kube M, Kostadinov I, Teeling H, Glockner FO, Reinhardt R, Amann R (2010)
Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group
Environ Microbiol
12 :422
Meyerdierks A, Kube M, Kostadinov I, Teeling H, Glockner FO, Reinhardt R, Amann R (2010)
Environ Microbiol
12 :422