Teeling H

References (10)

Title : Polysaccharide utilisation loci of Bacteroidetes from two contrasting open ocean sites in the North Atlantic - Bennke_2016_Environ.Microbiol_18_4456
Author(s) : Bennke CM , Kruger K , Kappelmann L , Huang S , Gobet A , Schuler M , Barbe V , Fuchs BM , Michel G , Teeling H , Amann RI
Ref : Environ Microbiol , 18 :4456 , 2016
Abstract : Marine Bacteroidetes have pronounced capabilities of degrading high molecular weight organic matter such as proteins and polysaccharides. Previously we reported on 76 Bacteroidetes-affiliated fosmids from the North Atlantic Ocean's boreal polar and oligotrophic subtropical provinces. Here, we report on the analysis of further 174 fosmids from the same libraries. The combined, re-assembled dataset (226 contigs; 8.8 Mbp) suggests that planktonic Bacteroidetes at the oligotrophic southern station use more peptides and bacterial and animal polysaccharides, whereas Bacteroidetes at the polar station (East-Greenland Current) use more algal and plant polysaccharides. The latter agrees with higher abundances of algae and terrigenous organic matter, including plant material, at the polar station. Results were corroborated by in-depth bioinformatic analysis of 14 polysaccharide utilisation loci from both stations, suggesting laminarin-specificity for four and specificity for sulfated xylans for two loci. In addition, one locus from the polar station supported use of non-sulfated xylans and mannans, possibly of plant origin. While peptides likely represent a prime source of carbon for Bacteroidetes in open oceans, our data suggest that as yet unstudied clades of these Bacteroidetes have a surprisingly broad capacity for polysaccharide degradation. In particular, laminarin-specific PULs seem widespread and thus must be regarded as globally important.
ESTHER : Bennke_2016_Environ.Microbiol_18_4456
PubMedSearch : Bennke_2016_Environ.Microbiol_18_4456
PubMedID: 27348854
Gene_locus related to this paper: 9bact-a0a1b2ypb1

Title : Genome Sequence of Thalassolituus oleivorans MIL-1 (DSM 14913T) - Golyshin_2013_Genome.Announc_1_e0014113
Author(s) : Golyshin PN , Werner J , Chernikova TN , Tran H , Ferrer M , Yakimov MM , Teeling H , Golyshina OV
Ref : Genome Announc , 1 :e0014113 , 2013
Abstract : Thalassolituus oleivorans is one of the most prevalent marine gammaproteobacteria in microbial communities, emerging after oil spills in coastal, estuarine, and surface seawaters. Here, we present the assembled genome of strain T. oleivorans MIL-1 (DSM 14913(T)), which is 3,920,328 bp with a G+C content of 46.6%.
ESTHER : Golyshin_2013_Genome.Announc_1_e0014113
PubMedSearch : Golyshin_2013_Genome.Announc_1_e0014113
PubMedID: 23599290
Gene_locus related to this paper: 9gamm-m5dq85 , 9gamm-m5dt68 , 9gamm-m5dm97

Title : The genome of the alga-associated marine flavobacterium Formosa agariphila KMM 3901T reveals a broad potential for degradation of algal polysaccharides - Mann_2013_Appl.Environ.Microbiol_79_6813
Author(s) : Mann AJ , Hahnke RL , Huang S , Werner J , Xing P , Barbeyron T , Huettel B , Stuber K , Reinhardt R , Harder J , Glockner FO , Amann RI , Teeling H
Ref : Applied Environmental Microbiology , 79 :6813 , 2013
Abstract : In recent years, representatives of the Bacteroidetes have been increasingly recognized as specialists for the degradation of macromolecules. Formosa constitutes a Bacteroidetes genus within the class Flavobacteria, and the members of this genus have been found in marine habitats with high levels of organic matter, such as in association with algae, invertebrates, and fecal pellets. Here we report on the generation and analysis of the genome of the type strain of Formosa agariphila (KMM 3901(T)), an isolate from the green alga Acrosiphonia sonderi. F. agariphila is a facultative anaerobe with the capacity for mixed acid fermentation and denitrification. Its genome harbors 129 proteases and 88 glycoside hydrolases, indicating a pronounced specialization for the degradation of proteins, polysaccharides, and glycoproteins. Sixty-five of the glycoside hydrolases are organized in at least 13 distinct polysaccharide utilization loci, where they are clustered with TonB-dependent receptors, SusD-like proteins, sensors/transcription factors, transporters, and often sulfatases. These loci play a pivotal role in bacteroidetal polysaccharide biodegradation and in the case of F. agariphila revealed the capacity to degrade a wide range of algal polysaccharides from green, red, and brown algae and thus a strong specialization of toward an alga-associated lifestyle. This was corroborated by growth experiments, which confirmed usage particularly of those monosaccharides that constitute the building blocks of abundant algal polysaccharides, as well as distinct algal polysaccharides, such as laminarins, xylans, and kappa-carrageenans.
ESTHER : Mann_2013_Appl.Environ.Microbiol_79_6813
PubMedSearch : Mann_2013_Appl.Environ.Microbiol_79_6813
PubMedID: 23995932
Gene_locus related to this paper: forag-t2ki26

Title : Genome and physiology of a model Epsilonproteobacterium responsible for sulfide detoxification in marine oxygen depletion zones - Grote_2012_Proc.Natl.Acad.Sci.U.S.A_109_506
Author(s) : Grote J , Schott T , Bruckner CG , Glockner FO , Jost G , Teeling H , Labrenz M , Jurgens K
Ref : Proc Natl Acad Sci U S A , 109 :506 , 2012
Abstract : Eutrophication and global climate change lead to expansion of hypoxia in the ocean, often accompanied by the production of hydrogen sulfide, which is toxic to higher organisms. Chemoautotrophic bacteria are thought to buffer against increased sulfide concentrations by oxidizing hydrogen sulfide before its diffusion to oxygenated surface waters. Model organisms from such environments have not been readily available, which has contributed to a poor understanding of these microbes. We present here a detailed study of "Sulfurimonas gotlandica" str. GD1, an Epsilonproteobacterium isolated from the Baltic Sea oxic-anoxic interface, where it plays a key role in nitrogen and sulfur cycling. Whole-genome analysis and laboratory experiments revealed a high metabolic flexibility, suggesting a considerable capacity for adaptation to variable redox conditions. S. gotlandica str. GD1 was shown to grow chemolithoautotrophically by coupling denitrification with oxidation of reduced sulfur compounds and dark CO(2) fixation. Metabolic versatility was further suggested by the use of a range of different electron donors and acceptors and organic carbon sources. The number of genes involved in signal transduction and metabolic pathways exceeds those of other Epsilonproteobacteria. Oxygen tolerance and environmental-sensing systems combined with chemotactic responses enable this organism to thrive successfully in marine oxygen-depletion zones. We propose that S. gotlandica str. GD1 will serve as a model organism in investigations that will lead to a better understanding how members of the Epsilonproteobacteria are able to cope with water column anoxia and the role these microorganisms play in the detoxification of sulfidic waters.
ESTHER : Grote_2012_Proc.Natl.Acad.Sci.U.S.A_109_506
PubMedSearch : Grote_2012_Proc.Natl.Acad.Sci.U.S.A_109_506
PubMedID: 22203982
Gene_locus related to this paper: sulgg-b6bk50 , sulgg-b6bjr0

Title : Fine-scale evolution: genomic, phenotypic and ecological differentiation in two coexisting Salinibacter ruber strains - Pena_2010_ISME.J_4_882
Author(s) : Pena A , Teeling H , Huerta-Cepas J , Santos F , Yarza P , Brito-Echeverria J , Lucio M , Schmitt-Kopplin P , Meseguer I , Schenowitz C , Dossat C , Barbe V , Dopazo J , Rossello-Mora R , Schuler M , Glockner FO , Amann R , Gabaldon T , Anton J
Ref : Isme J , 4 :882 , 2010
Abstract : Genomic and metagenomic data indicate a high degree of genomic variation within microbial populations, although the ecological and evolutive meaning of this microdiversity remains unknown. Microevolution analyses, including genomic and experimental approaches, are so far very scarce for non-pathogenic bacteria. In this study, we compare the genomes, metabolomes and selected ecological traits of the strains M8 and M31 of the hyperhalophilic bacterium Salinibacter ruber that contain ribosomal RNA (rRNA) gene and intergenic regions that are identical in sequence and were simultaneously isolated from a Mediterranean solar saltern. Comparative analyses indicate that S. ruber genomes present a mosaic structure with conserved and hypervariable regions (HVRs). The HVRs or genomic islands, are enriched in transposases, genes related to surface properties, strain-specific genes and highly divergent orthologous. However, the many indels outside the HVRs indicate that genome plasticity extends beyond them. Overall, 10% of the genes encoded in the M8 genome are absent from M31 and could stem from recent acquisitions. S. ruber genomes also harbor 34 genes located outside HVRs that are transcribed during standard growth and probably derive from lateral gene transfers with Archaea preceding the M8/M31 divergence. Metabolomic analyses, phage susceptibility and competition experiments indicate that these genomic differences cannot be considered neutral from an ecological perspective. The results point to the avoidance of competition by micro-niche adaptation and response to viral predation as putative major forces that drive microevolution within these Salinibacter strains. In addition, this work highlights the extent of bacterial functional diversity and environmental adaptation, beyond the resolution of the 16S rRNA and internal transcribed spacers regions.
ESTHER : Pena_2010_ISME.J_4_882
PubMedSearch : Pena_2010_ISME.J_4_882
PubMedID: 20164864
Gene_locus related to this paper: salrd-q2rzy1 , salrd-q2s0e4 , salrd-q2s1i8

Title : Metagenome and mRNA expression analyses of anaerobic methanotrophic archaea of the ANME-1 group - Meyerdierks_2010_Environ.Microbiol_12_422
Author(s) : Meyerdierks A , Kube M , Kostadinov I , Teeling H , Glockner FO , Reinhardt R , Amann R
Ref : Environ Microbiol , 12 :422 , 2010
Abstract : Microbial consortia mediating the anaerobic oxidation of methane with sulfate are composed of methanotrophic Archaea (ANME) and Bacteria related to sulfate-reducing Deltaproteobacteria. Cultured representatives are not available for any of the three ANME clades. Therefore, a metagenomic approach was applied to assess the genetic potential of ANME-1 archaea. In total, 3.4 Mbp sequence information was generated based on metagenomic fosmid libraries constructed directly from a methanotrophic microbial mat in the Black Sea. These sequence data represent, in 30 contigs, about 82-90% of a composite ANME-1 genome. The dataset supports the hypothesis of a reversal of the methanogenesis pathway. Indications for an assimilatory, but not for a dissimilatory sulfate reduction pathway in ANME-1, were found. Draft genome and expression analyses are consistent with acetate and formate as putative electron shuttles. Moreover, the dataset points towards downstream electron-accepting redox components different from the ones known from methanogenic archaea. Whereas catalytic subunits of [NiFe]-hydrogenases are lacking in the dataset, genes for an [FeFe]-hydrogenase homologue were identified, not yet described to be present in methanogenic archaea. Clustered genes annotated as secreted multiheme c-type cytochromes were identified, which have not yet been correlated with methanogenesis-related steps. The genes were shown to be expressed, suggesting direct electron transfer as an additional possible mode to shuttle electrons from ANME-1 to the bacterial sulfate-reducing partner.
ESTHER : Meyerdierks_2010_Environ.Microbiol_12_422
PubMedSearch : Meyerdierks_2010_Environ.Microbiol_12_422
PubMedID: 19878267
Gene_locus related to this paper: 9arch-d1j9b3 , 9arch-d1ja37 , 9arch-d1jaz1 , 9arch-q64ad0

Title : Characterization of a marine gammaproteobacterium capable of aerobic anoxygenic photosynthesis - Fuchs_2007_Proc.Natl.Acad.Sci.U.S.A_104_2891
Author(s) : Fuchs BM , Spring S , Teeling H , Quast C , Wulf J , Schattenhofer M , Yan S , Ferriera S , Johnson J , Glockner FO , Amann R
Ref : Proc Natl Acad Sci U S A , 104 :2891 , 2007
Abstract : Members of the gammaproteobacterial clade NOR5/OM60 regularly form an abundant part, up to 11%, of the bacterioplankton community in coastal systems during the summer months. Here, we report the nearly complete genome sequence of one cultured representative, Congregibacter litoralis strain KT71, isolated from North Sea surface water. Unexpectedly, a complete photosynthesis superoperon, including genes for accessory pigments, was discovered. It has a high sequence similarity to BAC clones from Monterey Bay [Beja O, Suzuki MT, Heidelberg JF, Nelson WC, Preston CM, et al. (2002) Nature 415:630-633], which also share a nearly identical gene arrangement. Although cultures of KT71 show no obvious pigmentation, bacteriochlorophyll a and spirilloxanthin-like carotenoids could be detected by HPLC analysis in cell extracts. The presence of two potential BLUF (blue light using flavin adenine dinucleotide sensors), one of which was found adjacent to the photosynthesis operon in the genome, indicates a light- and redox-dependent regulation of gene expression. Like other aerobic anoxygenic phototrophs (AAnPs), KT71 is able to grow neither anaerobically nor photoautotrophically. Cultivation experiments and genomic evidence show that KT71 needs organic substrates like carboxylic acids, oligopeptides, or fatty acids for growth. The strain grows optimally under microaerobic conditions and actively places itself in a zone of approximately 10% oxygen saturation. The genome analysis of C. litoralis strain KT71 identifies the gammaproteobacterial marine AAnPs, postulated based on BAC sequences, as members of the NOR5/OM60 clade. KT71 enables future experiments investigating the importance of this group of gammaproteobacterial AAnPs in coastal environments.
ESTHER : Fuchs_2007_Proc.Natl.Acad.Sci.U.S.A_104_2891
PubMedSearch : Fuchs_2007_Proc.Natl.Acad.Sci.U.S.A_104_2891
PubMedID: 17299055
Gene_locus related to this paper: 9gamm-a4a7a9 , 9gamm-a4a9u6 , 9gamm-a4a440 , 9gamm-a4a484 , 9gamm-a4a590 , 9gamm-a4a746 , 9gamm-a4a758 , 9gamm-a4aa23 , 9gamm-a4acc1 , 9gamm-a4ada0 , 9gamm-a4ae00 , 9gamm-a4acf4 , 9gamm-a4acr9

Title : Whole genome analysis of the marine Bacteroidetes'Gramella forsetii' reveals adaptations to degradation of polymeric organic matter - Bauer_2006_Environ.Microbiol_8_2201
Author(s) : Bauer M , Kube M , Teeling H , Richter M , Lombardot T , Allers E , Wurdemann CA , Quast C , Kuhl H , Knaust F , Woebken D , Bischof K , Mussmann M , Choudhuri JV , Meyer F , Reinhardt R , Amann RI , Glockner FO
Ref : Environ Microbiol , 8 :2201 , 2006
Abstract : Members of the Bacteroidetes, formerly known as the Cytophaga-Flavobacteria-Bacteroides (CFB) phylum, are among the major taxa of marine heterotrophic bacterioplankton frequently found on macroscopic organic matter particles (marine snow). In addition, they have been shown to also represent a significant part of free-living microbial assemblages in nutrient-rich microenvironments. Their abundance and distribution pattern in combination with enzymatic activity studies has led to the notion that organisms of this group are specialists for degradation of high molecular weight compounds in both the dissolved and particulate fraction of the marine organic matter pool, implying a major role of Bacteroidetes in the marine carbon cycle. Despite their ecological importance, comprehensive molecular data on organisms of this group have been scarce so far. Here we report on the first whole genome analysis of a marine Bacteroidetes representative, 'Gramella forsetii' KT0803. Functional analysis of the predicted proteome disclosed several traits which in joint consideration suggest a clear adaptation of this marine Bacteroidetes representative to the degradation of high molecular weight organic matter, such as a substantial suite of genes encoding hydrolytic enzymes, a predicted preference for polymeric carbon sources and a distinct capability for surface adhesion.
ESTHER : Bauer_2006_Environ.Microbiol_8_2201
PubMedSearch : Bauer_2006_Environ.Microbiol_8_2201
PubMedID: 17107561
Gene_locus related to this paper: grafk-a0ly58 , grafk-a0lyb1 , grafk-a0lzl9 , grafk-a0lzn2 , grafk-a0m3f2 , grafk-a0m3h3 , grafk-a0m3u0 , grafk-a0m4n9 , grafk-a0m5m4 , grafk-a0m6x9 , grafk-a0m6z1 , grafk-a0m243 , grafk-a0m536 , grafk-a0m641 , grafk-a0m784 , grafk-a0m4i1

Title : The genome of Desulfotalea psychrophila, a sulfate-reducing bacterium from permanently cold Arctic sediments - Rabus_2004_Environ.Microbiol_6_887
Author(s) : Rabus R , Ruepp A , Frickey T , Rattei T , Fartmann B , Stark M , Bauer M , Zibat A , Lombardot T , Becker I , Amann J , Gellner K , Teeling H , Leuschner WD , Glockner FO , Lupas AN , Amann R , Klenk HP
Ref : Environ Microbiol , 6 :887 , 2004
Abstract : Desulfotalea psychrophila is a marine sulfate-reducing delta-proteobacterium that is able to grow at in situ temperatures below 0 degrees C. As abundant members of the microbial community in permanently cold marine sediments, D. psychrophila-like bacteria contribute to the global cycles of carbon and sulfur. Here, we describe the genome sequence of D. psychrophila strain LSv54, which consists of a 3 523 383 bp circular chromosome with 3118 predicted genes and two plasmids of 121 586 bp and 14 663 bp. Analysis of the genome gave insight into the metabolic properties of the organism, e.g. the presence of TRAP-T systems as a major route for the uptake of C(4)-dicarboxylates, the unexpected presence of genes from the TCA cycle, a TAT secretion system, the lack of a beta-oxidation complex and typical Desulfovibrio cytochromes, such as c(553), c(3) and ncc. D. psychrophila encodes more than 30 two-component regulatory systems, including a new Ntr subcluster of hybrid kinases, nine putative cold shock proteins and nine potentially cold shock-inducible proteins. A comparison of D. psychrophila's genome features with those of the only other published genome from a sulfate reducer, the hyperthermophilic archaeon Archaeoglobus fulgidus, revealed many striking differences, but only a few shared features.
ESTHER : Rabus_2004_Environ.Microbiol_6_887
PubMedSearch : Rabus_2004_Environ.Microbiol_6_887
PubMedID: 15305914
Gene_locus related to this paper: desps-q6ajw5 , desps-q6ak51 , desps-q6ak75 , desps-q6akv7 , desps-q6and7 , desps-q6anv2 , desps-q6ar42 , desps-q6ara4

Title : Complete genome sequence of the marine planctomycete Pirellula sp. strain 1 - Glockner_2003_Proc.Natl.Acad.Sci.U.S.A_100_8298
Author(s) : Glockner FO , Kube M , Bauer M , Teeling H , Lombardot T , Ludwig W , Gade D , Beck A , Borzym K , Heitmann K , Rabus R , Schlesner H , Amann R , Reinhardt R
Ref : Proc Natl Acad Sci U S A , 100 :8298 , 2003
Abstract : Pirellula sp. strain 1 ("Rhodopirellula baltica") is a marine representative of the globally distributed and environmentally important bacterial order Planctomycetales. Here we report the complete genome sequence of a member of this independent phylum. With 7.145 megabases, Pirellula sp. strain 1 has the largest circular bacterial genome sequenced so far. The presence of all genes required for heterolactic acid fermentation, key genes for the interconversion of C1 compounds, and 110 sulfatases were unexpected for this aerobic heterotrophic isolate. Although Pirellula sp. strain 1 has a proteinaceous cell wall, remnants of genes for peptidoglycan synthesis were found. Genes for lipid A biosynthesis and homologues to the flagellar L- and P-ring protein indicate a former Gram-negative type of cell wall. Phylogenetic analysis of all relevant markers clearly affiliates the Planctomycetales to the domain Bacteria as a distinct phylum, but a deepest branching is not supported by our analyses.
ESTHER : Glockner_2003_Proc.Natl.Acad.Sci.U.S.A_100_8298
PubMedSearch : Glockner_2003_Proc.Natl.Acad.Sci.U.S.A_100_8298
PubMedID: 12835416
Gene_locus related to this paper: rhoba-DHLA , rhoba-EPHA , rhoba-pepx , rhoba-PLDB , rhoba-q7tty3 , rhoba-q7ufy7 , rhoba-q7ug04 , rhoba-q7uh76 , rhoba-q7uhj7 , rhoba-q7uik0 , rhoba-q7uit3 , rhoba-q7ujb6 , rhoba-q7ujc7.1 , rhoba-q7ujc7.2 , rhoba-q7ujd0 , rhoba-q7ujp7 , rhoba-q7ujv7 , rhoba-q7uk55 , rhoba-q7uk83 , rhoba-q7ul52 , rhoba-q7ule9 , rhoba-q7umh7 , rhoba-q7upt9 , rhoba-q7uq60 , rhoba-q7uqf1 , rhoba-q7uqr5 , rhoba-q7uqz0 , rhoba-q7ur17 , rhoba-q7ur85 , rhoba-q7urv6 , rhoba-q7us57 , rhoba-q7usu9 , rhoba-q7ut87 , rhoba-q7ut89 , rhoba-q7utj6 , rhoba-q7uua2 , rhoba-q7uvu6 , rhoba-q7uw49 , rhoba-q7uw53 , rhoba-q7uwf0 , rhoba-q7uwf1 , rhoba-q7uxn0 , rhoba-q7uyb7 , rhoba-q7uyh2 , rhoba-q7uz96 , rhoba-RB3694 , rhoba-RB4467 , rhoba-RB7907 , rhoba-RB9080 , rhoba-RB9565 , rhoba-RB13257