Title : Assigning Functions of Unknown Enzymes by High-Throughput Enzyme Characterization - Molina-Espeja_2023_Methods.Mol.Biol_2555_181 |
Author(s) : Molina-Espeja P , Fernandez-Lopez L , Golyshin PN , Ferrer M |
Ref : Methods Mol Biol , 2555 :181 , 2023 |
Abstract :
The discovery of new enzymes is strongly enabled by the implementation of high-throughput screening methods to detect enzymatic activity in single organisms or clone expression libraries, or to benchmark their performances against known prototypes. In this chapter, a number of methods, applicable at high-throughput scale, are described that allow the screening and characterization of enzymes relevant to biotechnology, particularly, ester-hydrolases (esterases, lipases, phospholipases, and polyester hydrolases). |
PubMedSearch : Molina-Espeja_2023_Methods.Mol.Biol_2555_181 |
PubMedID: 36306087 |
Molina-Espeja P, Fernandez-Lopez L, Golyshin PN, Ferrer M (2023)
Assigning Functions of Unknown Enzymes by High-Throughput Enzyme Characterization
Methods Mol Biol
2555 :181
Molina-Espeja P, Fernandez-Lopez L, Golyshin PN, Ferrer M (2023)
Methods Mol Biol
2555 :181