Nolling_2001_J.Bacteriol_183_4823

Reference

Title : Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum - Nolling_2001_J.Bacteriol_183_4823
Author(s) : Nolling J , Breton G , Omelchenko MV , Makarova KS , Zeng Q , Gibson R , Lee HM , Dubois J , Qiu D , Hitti J , Wolf YI , Tatusov RL , Sabathe F , Doucette-Stamm L , Soucaille P , Daly MJ , Bennett GN , Koonin EV , Smith DR
Ref : Journal of Bacteriology , 183 :4823 , 2001
Abstract :

The genome sequence of the solvent-producing bacterium Clostridium acetobutylicum ATCC 824 has been determined by the shotgun approach. The genome consists of a 3.94-Mb chromosome and a 192-kb megaplasmid that contains the majority of genes responsible for solvent production. Comparison of C. acetobutylicum to Bacillus subtilis reveals significant local conservation of gene order, which has not been seen in comparisons of other genomes with similar, or, in some cases closer, phylogenetic proximity. This conservation allows the prediction of many previously undetected operons in both bacteria. However, the C. acetobutylicum genome also contains a significant number of predicted operons that are shared with distantly related bacteria and archaea but not with B. subtilis. Phylogenetic analysis is compatible with the dissemination of such operons by horizontal transfer. The enzymes of the solventogenesis pathway and of the cellulosome of C. acetobutylicum comprise a new set of metabolic capacities not previously represented in the collection of complete genomes. These enzymes show a complex pattern of evolutionary affinities, emphasizing the role of lateral gene exchange in the evolution of the unique metabolic profile of the bacterium. Many of the sporulation genes identified in B. subtilis are missing in C. acetobutylicum, which suggests major differences in the sporulation process. Thus, comparative analysis reveals both significant conservation of the genome organization and pronounced differences in many systems that reflect unique adaptive strategies of the two gram-positive bacteria.

PubMedSearch : Nolling_2001_J.Bacteriol_183_4823
PubMedID: 11466286
Gene_locus related to this paper: cloab-CAC2917 , cloab-q97db4 , cloab-q97fl1 , cloac-CAC0202 , cloac-CAC0719 , cloac-CAC0816 , cloac-CAC1022 , cloac-CAC1028 , cloac-CAC1450 , cloac-CAC1470 , cloac-CAC1962 , cloac-CAC2246 , cloac-CAC2472 , cloac-CAC2688 , cloac-CAC2936 , cloac-CAC3022 , cloac-CAC3402 , cloac-CAC3407 , cloac-CAC3432 , cloac-CAC3515 , cloac-CAC3665 , cloac-CAP0071 , cloac-CAP0097 , cloac-CAP0133 , cloac-pnbae

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Citations formats

Nolling J, Breton G, Omelchenko MV, Makarova KS, Zeng Q, Gibson R, Lee HM, Dubois J, Qiu D, Hitti J, Wolf YI, Tatusov RL, Sabathe F, Doucette-Stamm L, Soucaille P, Daly MJ, Bennett GN, Koonin EV, Smith DR (2001)
Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum
Journal of Bacteriology 183 :4823

Nolling J, Breton G, Omelchenko MV, Makarova KS, Zeng Q, Gibson R, Lee HM, Dubois J, Qiu D, Hitti J, Wolf YI, Tatusov RL, Sabathe F, Doucette-Stamm L, Soucaille P, Daly MJ, Bennett GN, Koonin EV, Smith DR (2001)
Journal of Bacteriology 183 :4823