Title : Crystallographic Study of Mutants and Complexes of Oligopeptidase B from Serratia proteamaculans - Petrenko_2020_Crystallogr.Rep_65_909 |
Author(s) : Petrenko DE , Nikolaeva AY , Lazarenko VA , Dorovatovskiy PV , Timofeev VI , Vlaskina AV , Korzhenevskiy DA , Mikhailova AG , Boyko KM , Rakitina TV |
Ref : Crystallogr Rep , 65 :909 , 2020 |
Abstract :
The results of the structural studies of bacterial oligopeptidase B (OpB) belonging to the least well-studied prolyl oligopeptidase family are described. The screening of crystallization conditions for mutants of the enzyme, its complexes with peptides, which mimic substrates and catalytic reaction products, and a complex with a transition state analog as an inhibitor was performed in order to determine the three-dimensional structures of OpB from Serratia proteamaculans (PSP) acting at different steps of the catalytic cycle. Crystals suitable for X-ray diffraction were grown. The X-ray diffraction data sets were collected, processed, and subjected to preliminary analysis. These X-ray diffraction data sets are suitable for obtaining the structural data necessary for the description of the catalytic cycle of bacterial OpBs. |
PubMedSearch : Petrenko_2020_Crystallogr.Rep_65_909 |
PubMedID: |
Gene_locus related to this paper: 9gamm-b3vi58 |
Gene_locus | 9gamm-b3vi58 |
Petrenko DE, Nikolaeva AY, Lazarenko VA, Dorovatovskiy PV, Timofeev VI, Vlaskina AV, Korzhenevskiy DA, Mikhailova AG, Boyko KM, Rakitina TV (2020)
Crystallographic Study of Mutants and Complexes of Oligopeptidase B from Serratia proteamaculans
Crystallogr Rep
65 :909
Petrenko DE, Nikolaeva AY, Lazarenko VA, Dorovatovskiy PV, Timofeev VI, Vlaskina AV, Korzhenevskiy DA, Mikhailova AG, Boyko KM, Rakitina TV (2020)
Crystallogr Rep
65 :909