Pinotsis_2023_Nat.Commun_14_7649

Reference

Title : Discovery of a non-canonical prototype long-chain monoacylglycerol lipase through a structure-based endogenous reaction intermediate complex - Pinotsis_2023_Nat.Commun_14_7649
Author(s) : Pinotsis N , Kruger A , Tomas N , Chatziefthymiou SD , Litz C , Mortensen SA , Daffe M , Marrakchi H , Antranikian G , Wilmanns M
Ref : Nat Commun , 14 :7649 , 2023
Abstract :

The identification and characterization of enzyme function is largely lacking behind the rapidly increasing availability of large numbers of sequences and associated high-resolution structures. This is often hampered by lack of knowledge on in vivo relevant substrates. Here, we present a case study of a high-resolution structure of an unusual orphan lipase in complex with an endogenous C18 monoacylglycerol ester reaction intermediate from the expression host, which is insoluble under aqueous conditions and thus not accessible for studies in solution. The data allowed its functional characterization as a prototypic long-chain monoacylglycerol lipase, which uses a minimal lid domain to position the substrate through a hydrophobic tunnel directly to the enzyme's active site. Knowledge about the molecular details of the substrate binding site allowed us to modulate the enzymatic activity by adjusting protein/substrate interactions, demonstrating the potential of our findings for future biotechnology applications.

PubMedSearch : Pinotsis_2023_Nat.Commun_14_7649
PubMedID: 38012138

Related information

Inhibitor PMSF
Substrate PMSF    Monostearin    Monooctanoin
Family PMSF    Monostearin    Monooctanoin    FAE-Bacterial-promiscuous
Structure PMSF    Monostearin    Monooctanoin    FAE-Bacterial-promiscuous    7Q4H    8B9S    7Q4J

Citations formats

Pinotsis N, Kruger A, Tomas N, Chatziefthymiou SD, Litz C, Mortensen SA, Daffe M, Marrakchi H, Antranikian G, Wilmanns M (2023)
Discovery of a non-canonical prototype long-chain monoacylglycerol lipase through a structure-based endogenous reaction intermediate complex
Nat Commun 14 :7649

Pinotsis N, Kruger A, Tomas N, Chatziefthymiou SD, Litz C, Mortensen SA, Daffe M, Marrakchi H, Antranikian G, Wilmanns M (2023)
Nat Commun 14 :7649