Title : Construction of adenovirus for high level expression of small RNAs in mammalian cells. Application to a Bcl-2 ribozyme - Potter_2000_Mol.Biotechnol_15_105 |
Author(s) : Potter PM , McKenzie PP , Hussain N , Noonberg S , Morton CL , Harris LC |
Ref : Mol Biotechnol , 15 :105 , 2000 |
Abstract :
A series of plasmid vectors have been generated to allow the rapid construction of adenoviral vectors designed to express small RNA sequences. A truncated human U6 gene containing convenient restriction sites has been shown to be expressed at high levels following electroporation into a series of human cell lines. This gene was ligated into a promoterless adenoviral plasmid, and we have generated high titer virus by homologous recombination with adenoviral Addl327 DNA in 293 cells. Recombinant adenovirus containing a hammerhead ribozyme sequence targeted toward the Bcl-2 mRNA has been used to transduce a panel of human tumor cell lines. We have demonstrated high level expression of the recombinant U6 gene containing the ribozyme and reduction of Bcl-2 protein in transduced cells. These plasmids are suitable for the development of adenoviral vectors designed to express both ribozymes and antisense RNA in human cells. |
PubMedSearch : Potter_2000_Mol.Biotechnol_15_105 |
PubMedID: 10949823 |
Potter PM, McKenzie PP, Hussain N, Noonberg S, Morton CL, Harris LC (2000)
Construction of adenovirus for high level expression of small RNAs in mammalian cells. Application to a Bcl-2 ribozyme
Mol Biotechnol
15 :105
Potter PM, McKenzie PP, Hussain N, Noonberg S, Morton CL, Harris LC (2000)
Mol Biotechnol
15 :105