Title : FSH1 encodes lysophospholipase activity in Saccharomyces cerevisiae - Ramachandran_2021_Biotechnol.Lett_43_279 |
Author(s) : Ramachandran G , Chidambaram R , Nachiappan V |
Ref : Biotechnol Lett , 43 :279 , 2021 |
Abstract :
OBJECTIVES: To elucidate the role of FSH1 (family of serine hydrolase) in lipid homeostasis. RESULTS: Proteins in various species containing alpha/beta hydrolase domain are known to be involved in lipid metabolism. In silico analysis of the FSH1 gene in Saccharomyces cerevisiae revealed the presence of alpha/beta hydrolase domain (ABHD) and a lipase motif (GXSXG), however its function in lipid metabolism remained elusive. The overexpression of FSH1 in WT and fsh1delta cells showed a significant reduction in the cellular phospholipid levels and an increase in the triacylglycerol levels and lipid droplet (LD) number. Furthermore, the purified recombinant protein Fsh1p was identified as a lysophospholipase that specifically acts on lysophosphatidylserine (LPS) and impacts the lipid homeostasis in S. cerevisiae. CONCLUSIONS: These results depicted that Fsh1p has a role on lipid homeostasis and is a lysophospholipase that hydrolyzes lysophosphatidylserine (LPS). |
PubMedSearch : Ramachandran_2021_Biotechnol.Lett_43_279 |
PubMedID: 32920715 |
Gene_locus related to this paper: yeast-FSH1 |
Gene_locus | yeast-FSH1 |
Family | FSH1 |
Ramachandran G, Chidambaram R, Nachiappan V (2021)
FSH1 encodes lysophospholipase activity in Saccharomyces cerevisiae
Biotechnol Lett
43 :279
Ramachandran G, Chidambaram R, Nachiappan V (2021)
Biotechnol Lett
43 :279