Roppongi_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_601

Reference

Title : Periplasmic form of dipeptidyl aminopeptidase IV from Pseudoxanthomonas mexicana WO24: purification, kinetic characterization, crystallization and X-ray crystallographic analysis - Roppongi_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_601
Author(s) : Roppongi S , Tateoka C , Fujimoto M , Iizuka I , Morisawa S , Nakamura A , Honma N , Suzuki Y , Shida Y , Ogasawara W , Tanaka N , Sakamoto Y , Nonaka T
Ref : Acta Crystallographica F Struct Biol Commun , 73 :601 , 2017
Abstract :

Dipeptidyl aminopeptidase IV (DAP IV or DPP IV) from Pseudoxanthomonas mexicana WO24 (PmDAP IV) preferentially cleaves substrate peptides with Pro or Ala at the P1 position [NH2-P2-P1(Pro/Ala)-P1'-P2'...]. For crystallographic studies, the periplasmic form of PmDAP IV was overproduced in Escherichia coli, purified and crystallized in complex with the tripeptide Lys-Pro-Tyr using the hanging-drop vapour-diffusion method. Kinetic parameters of the purified enzyme against a synthetic substrate were also determined. X-ray diffraction data to 1.90 A resolution were collected from a triclinic crystal form belonging to space group P1, with unit-cell parameters a = 88.66, b = 104.49, c = 112.84 A, alpha = 67.42, beta = 68.83, gamma = 65.46 degrees . Initial phases were determined by the molecular-replacement method using Stenotrophomonas maltophilia DPP IV (PDB entry 2ecf) as a template and refinement of the structure is in progress.

PubMedSearch : Roppongi_2017_Acta.Crystallogr.F.Struct.Biol.Commun_73_601
PubMedID: 29095153
Gene_locus related to this paper: 9psed-q6f3i7

Related information

Inhibitor Ile-Pro    5YP2-8YC
Gene_locus Ile-Pro    5YP2-8YC    9psed-q6f3i7
Structure Ile-Pro    5YP2-8YC    9psed-q6f3i7    5YP1    5YP4    5YP3    5YP2

Citations formats

Roppongi S, Tateoka C, Fujimoto M, Iizuka I, Morisawa S, Nakamura A, Honma N, Suzuki Y, Shida Y, Ogasawara W, Tanaka N, Sakamoto Y, Nonaka T (2017)
Periplasmic form of dipeptidyl aminopeptidase IV from Pseudoxanthomonas mexicana WO24: purification, kinetic characterization, crystallization and X-ray crystallographic analysis
Acta Crystallographica F Struct Biol Commun 73 :601

Roppongi S, Tateoka C, Fujimoto M, Iizuka I, Morisawa S, Nakamura A, Honma N, Suzuki Y, Shida Y, Ogasawara W, Tanaka N, Sakamoto Y, Nonaka T (2017)
Acta Crystallographica F Struct Biol Commun 73 :601