Sagong_2020_ACS.Catal_10_4805

Reference

Title : Decomposition of the PET Film by MHETase Using Exo-PETase Function - Sagong_2020_ACS.Catal_10_48050
Author(s) : Sagong HY , Seo H , Kim T , Son H , Joo S , Lee S , Kim S , Woo JS , Hwang S , Kim KJ
Ref : ACS Catal , 10 :4805 , 2020
Abstract : Monohydroxyethyl terephthalate (MHET) hydrolase (MHETase) is an enzyme known to be involved in the final degradation step of poly(ethylene terephthalate) (PET) by hydrolyzing MHET into terephthalic acid and ethylene glycol in Ideonella sakaiensis. Here, we report the extracellular production of MHETase in an active form with a proper folding. Based on the structural observations and biochemical experiments, we reveal that MHETase also functions as exo-PETase by hydrolyzing the synthesized PET pentamer. We further present that MHETase has a hydrolysis activity against the termini-generated PET film, demonstrating the exo-PETase function of the enzyme. We also develop a MHETase R411K/S416A/F424I variant with a higher BHET activity, and the variant exhibits an enhanced degradation activity against the PET film. Based on these results, we propose that MHETase plays several roles in the biodegradation of PET using the BHETase and exo-PETase activities as well as the MHET hydrolysis function
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Gene_locus related to this paper: idesa-mheth

Related information

Gene_locus related to this paper: idesa-mheth

Citations formats

Sagong HY, Seo H, Kim T, Son H, Joo S, Lee S, Kim S, Woo JS, Hwang S, Kim KJ (2020)
Decomposition of the PET Film by MHETase Using Exo-PETase Function
ACS Catal 10 :4805

Sagong HY, Seo H, Kim T, Son H, Joo S, Lee S, Kim S, Woo JS, Hwang S, Kim KJ (2020)
ACS Catal 10 :4805