Scaps_1997_Arch.Environ.Contam.Toxicol_33_203

Reference

Title : Effects of organophosphate and carbamate pesticides on acetylcholinesterase and choline acetyltransferase activities of the polychaete Nereis diversicolor - Scaps_1997_Arch.Environ.Contam.Toxicol_33_203
Author(s) : Scaps P , Demuynck S , Descamps M , Dhainaut A
Ref : Archives of Environmental Contamination & Toxicology , 33 :203 , 1997
Abstract : A toxicity test for organophosphates (OP) and carbamates (C) was improved with the adult ragworm Nereis diversicolor. Animals were maintained in U-shaped glass tubes of 4-mm inner diameter fixed vertically on a plastic plate and placed in glass aquaria. Each tank was covered with glass in order to reduce evaporation and heat dissipation. Temperature varied between 15 and 16 degrees C and salinity was constant (34 per thousand) during the entire length of the experiment. Experiments were performed with a fixed day length of 12 h and seawater was gently aerated. The maintenance system allowed the administration of OP and C compounds via the seawater. An acclimatization period of 48 h was not sufficient to accomodate worms to their artificial burrows; accordingly, we chose to acclimate worms for a week before beginning the exposure. Choline acetyltransferase (ChAT) activity was very low and was not significantly modified by two OP compounds: malathion and parathion-ethyl. ChAT is not a target for these pesticides and should not be used for future studies about OP and C toxicity. On the other hand, inhibitory effects on acetylcholinesterase (AChE) activity were determined at concentrations of 10(-6) M for three OP compounds-malathion, parathion-ethyl, and phosalone-and a carbamate pesticide-carbaryl. We measured only short-term effects and no cumulative effect was determined, the maximum percentage of AChE activity inhibition being between 2 (carbaryl) and 7 (OP compounds) days after exposure and then remaining stable. Mortality occured only after a period of intoxication of 14 days. N diversicolor, which can be easily maintained at the laboratory, seems to be a good candidate for future laboratory studies to test the toxicity of other pollutants.
ESTHER : Scaps_1997_Arch.Environ.Contam.Toxicol_33_203
PubMedSearch : Scaps_1997_Arch.Environ.Contam.Toxicol_33_203
PubMedID: 9294250

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Citations formats

Scaps P, Demuynck S, Descamps M, Dhainaut A (1997)
Effects of organophosphate and carbamate pesticides on acetylcholinesterase and choline acetyltransferase activities of the polychaete Nereis diversicolor
Archives of Environmental Contamination & Toxicology 33 :203

Scaps P, Demuynck S, Descamps M, Dhainaut A (1997)
Archives of Environmental Contamination & Toxicology 33 :203