| Title : The hit-and-return system enables efficient time-resolved serial synchrotron crystallography - Schulz_2018_Nat.Methods_15_901 |
| Author(s) : Schulz EC , Mehrabi P , Muller-Werkmeister HM , Tellkamp F , Jha A , Stuart W , Persch E , De Gasparo R , Diederich F , Pai EF , Miller RJD |
| Ref : Nat Methods , 15 :901 , 2018 |
|
Abstract :
We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals. |
| PubMedSearch : Schulz_2018_Nat.Methods_15_901 |
| PubMedID: 30377366 |
| Gene_locus related to this paper: rhopa-q6nam1 |
| Substrate | Fluoroacetate |
| Gene_locus | rhopa-q6nam1 |
| Structure | 6GXH 6GXT 6GXL 6GXF 6GXD 6FSX |
Schulz EC, Mehrabi P, Muller-Werkmeister HM, Tellkamp F, Jha A, Stuart W, Persch E, De Gasparo R, Diederich F, Pai EF, Miller RJD (2018)
The hit-and-return system enables efficient time-resolved serial synchrotron crystallography
Nat Methods
15 :901
Schulz EC, Mehrabi P, Muller-Werkmeister HM, Tellkamp F, Jha A, Stuart W, Persch E, De Gasparo R, Diederich F, Pai EF, Miller RJD (2018)
Nat Methods
15 :901