Shan_2020_Int.J.Mol.Sci_21_

Reference

Title : Association of alpha\/beta-Hydrolase D16B with Bovine Conception Rate and Sperm Plasma Membrane Lipid Composition - Shan_2020_Int.J.Mol.Sci_21_
Author(s) : Shan S , Xu F , Bleyer M , Becker S , Melbaum T , Wemheuer W , Hirschfeld M , Wacker C , Zhao S , Schutz E , Brenig B
Ref : Int J Mol Sci , 21 : , 2020
Abstract :

We have identified a Holstein sire named Tarantino who had been approved for artificial insemination that is based on normal semen characteristics (i.e., morphology, thermoresistance, motility, sperm concentration), but had no progeny after 412 first inseminations, resulting in a non-return rate (NRdev) of -29. Using whole genome association analysis and next generation sequencing, an associated nonsense variant in the alpha/beta-hydrolase domain-containing 16B gene (ABHD16B) on bovine chromosome 13 was identified. The frequency of the mutant allele in the German Holstein population was determined to be 0.0018 in 222,645 investigated cattle specimens. The mutant allele was traced back to Whirlhill Kingpin (bornFeb. 13th, 1959) as potential founder. The expression of ABHD16B was detected by Western blotting and immunohistochemistry in testis and epididymis of control bulls. A lipidome comparison of the plasma membrane of fresh semen from carriers and controls showed significant differences in the concentration of phosphatidylcholine (PC), diacylglycerol (DAG), ceramide (Cer), sphingomyelin (SM), and phosphatidylcholine (-ether) (PC O-), indicating that ABHD16B plays a role in lipid biosynthesis. The altered lipid contents may explain the reduced fertilization ability of mutated sperms.

PubMedSearch : Shan_2020_Int.J.Mol.Sci_21_
PubMedID: 31963602
Gene_locus related to this paper: bovin-ABHD16B , human-ABHD16B , mouse-Abhd16b

Related information

Gene_locus bovin-ABHD16B    human-ABHD16B    mouse-Abhd16b
Family ABHD16

Citations formats

Shan S, Xu F, Bleyer M, Becker S, Melbaum T, Wemheuer W, Hirschfeld M, Wacker C, Zhao S, Schutz E, Brenig B (2020)
Association of alpha\/beta-Hydrolase D16B with Bovine Conception Rate and Sperm Plasma Membrane Lipid Composition
Int J Mol Sci 21 :

Shan S, Xu F, Bleyer M, Becker S, Melbaum T, Wemheuer W, Hirschfeld M, Wacker C, Zhao S, Schutz E, Brenig B (2020)
Int J Mol Sci 21 :