Shi_2024_J.Virol_e0127724

Reference

Title : Swine RNF5 positively regulates the antiviral activity of IFITM1 by mediating the degradation of ABHD16A - Shi_2024_J.Virol_e0127724
Author(s) : Shi X , , Shen L , , Chen S , , Liu M , , Wang J , , Wen X , , Liu W , , Mao L , , Ding Y , , Yu L , , Xu J
Ref : J Virol , :e0127724 , 2024
Abstract :

Interferon-inducible transmembrane (IFITM) proteins are broad-spectrum antiviral factors that confer cellular resistance to virus invasion. +/-/-Hydrolase domain-containing 16A (ABHD16A) has recently been identified as a novel depalmitoylase that can inhibit the antiviral activity of IFITM proteins by catalyzing the depalmitoyl reaction; this pattern may be crucial for the host to avoid damage caused by excessive immune response. However, it remains largely elusive about how host cells regulate the activity of ABHD16A. In the present study, we performed the AlphaFold2-based protein-protein interaction prediction and identified swine E3 ubiquitin ligase ring finger protein 5 (sRNF5) as a sABHD16A-interacting protein and negatively regulated the stability of sABHD16A. Using immunofluorescence and co-immunoprecipitation techniques, we uncovered that sRNF5 targeted sABHD16A for ubiquitination and degradation via the proteasomal pathway at residues K3 and K452. Furthermore, sABHD16A catalyzed the depalmitoylation of sIFITM1, which obstructed the antiviral function of sIFITM1, while sRNF5 caused ubiquitination of sABHD16A, which attenuated the depalmitoylation effect on sIFITM1, and consequently restored the antiviral activity of sIFITM1. Collectively, our findings demonstrate for the first time that sRNF5 positively regulates the antiviral function of sIFITM1 by mediating the degradation of sABHD16A, which expands the biological functions of RNF5 and ABHD16A in immune regulation. Moreover, our work highlights the well-designed interplay between RNF5, ABHD16A, and IFITM, which balances antiviral immune responses to avoid the disorders induced by excessive immune response.

PubMedSearch : Shi_2024_J.Virol_e0127724
PubMedID: 39601593
Gene_locus related to this paper: human-ABHD16A , mouse-Abhd16a , pig-a0a191tc51

Related information

Gene_locus human-ABHD16A    mouse-Abhd16a    pig-a0a191tc51
Family ABHD16

Citations formats

Shi X, , Shen L, , Chen S, , Liu M, , Wang J, , Wen X, , Liu W, , Mao L, , Ding Y, , Yu L, , Xu J (2024)
Swine RNF5 positively regulates the antiviral activity of IFITM1 by mediating the degradation of ABHD16A
J Virol :e0127724

Shi X, , Shen L, , Chen S, , Liu M, , Wang J, , Wen X, , Liu W, , Mao L, , Ding Y, , Yu L, , Xu J (2024)
J Virol :e0127724