Shih_2010_J.Mol.Neurosci_40_63

Reference

Title : Treatment with tertiary oximes prevents seizures and improves survival following sarin intoxication - Shih_2010_J.Mol.Neurosci_40_63
Author(s) : Shih TM , Skovira JW , O'Donnell JC , McDonough JH
Ref : Journal of Molecular Neuroscience , 40 :63 , 2010
Abstract :

The capability of the tertiary oximes, monoisonitrosoacetone (MINA) and diacetylmonoxime (DAM), to reactivate acetylcholinesterase (AChE) inhibited by sarin (GB) in the blood, brain, and peripheral tissues of guinea pigs was compared with that of the quaternary oximes 2-PAM, HLo7, and MMB-4. Animals were injected subcutaneously (s.c.) with 1.0 x LD(50) of GB and treated intramuscularly (i.m.) 5 min later with one of these oximes. Sixty minutes after GB exposure, tissues were collected for AChE analysis. At low doses, MINA and DAM produced significant increases in AChE activity in all brain areas examined, but no significant AChE reactivation in peripheral tissues or blood. At higher doses, MINA and DAM increased AChE activity in the brain, peripheral tissues, and blood. In contrast, the quaternary oximes produced significant reactivation in peripheral tissues and blood AChE, but no significant reactivation of brain AChE. In another study, animals were pretreated i.m. with pyridostigmine 30 min prior to s.c. challenge with 2.0 x LD(50) of GB and treated i.m. 1 min later with atropine sulfate (2.0 mg/kg), plus a varied dose of oximes. MINA and DAM prevented or terminated GB-induced seizure activity and protected against GB lethality in a dose-dependent fashion. In contrast, none of the quaternary oximes prevented or stopped GB-induced seizures. Thus, tertiary oximes reactivated AChE in the brain, improved survival, and terminated seizures following GB intoxication.

PubMedSearch : Shih_2010_J.Mol.Neurosci_40_63
PubMedID: 19680820

Related information

Reactivator MINA    DAM

Citations formats

Shih TM, Skovira JW, O'Donnell JC, McDonough JH (2010)
Treatment with tertiary oximes prevents seizures and improves survival following sarin intoxication
Journal of Molecular Neuroscience 40 :63

Shih TM, Skovira JW, O'Donnell JC, McDonough JH (2010)
Journal of Molecular Neuroscience 40 :63