| Title : Improved electrochemical analysis of neuropathy target esterase activity by a tyrosinase carbon paste electrode modified by 1-methoxyphenazine methosulfate - Sokolovskaya_2005_Biotechnol.Lett_27_1211 |
| Author(s) : Sokolovskaya LG , Sigolaeva LV , Eremenko AV , Gachok IV , Makhaeva GF , Strakhova NN , Malygin VV , Richardson RJ , Kurochkin IN |
| Ref : Biotechnol Lett , 27 :1211 , 2005 |
|
Abstract :
A graphite-paste tyrosinase biosensor was improved by adding 1-methoxyphenazine methosulfate as a mediator. Mediator modification enhanced sensitivity to phenol 4-fold and long-term stability 3-fold. Phenol could be detected at 25 nM (S/N = 2) using an Ag/AgCl reference electrode. The biosensor was used to measure the activity of a toxicologically significant enzyme, neuropathy target esterase (NTE), which yields phenol by hydrolysis of the substrate, phenyl valerate. Using the new biosensor, blood and brain NTE inhibition by organophosphorus (OP) compounds with different neuropathic potencies were well correlated (r = 0.990, n = 7), supporting the use of blood NTE as a biochemical marker of exposure to neuropathic OP compounds. |
| PubMedSearch : Sokolovskaya_2005_Biotechnol.Lett_27_1211 |
| PubMedID: 16158266 |
Sokolovskaya LG, Sigolaeva LV, Eremenko AV, Gachok IV, Makhaeva GF, Strakhova NN, Malygin VV, Richardson RJ, Kurochkin IN (2005)
Improved electrochemical analysis of neuropathy target esterase activity by a tyrosinase carbon paste electrode modified by 1-methoxyphenazine methosulfate
Biotechnol Lett
27 :1211
Sokolovskaya LG, Sigolaeva LV, Eremenko AV, Gachok IV, Makhaeva GF, Strakhova NN, Malygin VV, Richardson RJ, Kurochkin IN (2005)
Biotechnol Lett
27 :1211