Song_2015_Chem.Sci_6_4837

Reference

Title : Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae - Song_2015_Chem.Sci_6_4837
Author(s) : Song Z , Bakeer W , Marshall JW , Yakasai AA , Khalid RM , Collemare J , Skellam E , Tharreau D , Lebrun MH , Lazarus CM , Bailey AM , Simpson TJ , Cox RJ
Ref : Chem Sci , 6 :4837 , 2015
Abstract :

The ACE1 and RAP1 genes from the avirulence signalling gene cluster of the rice blast fungus Magnaporthe oryzae were expressed in Aspergillus oryzae and M. oryzae itself. Expression of ACE1 alone produced a polyenyl pyrone (magnaporthepyrone), which is regioselectively epoxidised and hydrolysed to give different diols, 6 and 7, in the two host organisms. Analysis of the three introns present in ACE1 determined that A. oryzae does not process intron 2 correctly, while M. oryzae processes all introns correctly in both appressoria and mycelia. Co-expression of ACE1 and RAP1 in A. oryzae produced an amide 8 which is similar to the PKS-NRPS derived backbone of the cytochalasans. Biological testing on rice leaves showed that neither the diols 6 and 7, nor amide 8 was responsible for the observed ACE1 mediated avirulence, however, gene cluster analysis suggests that the true avirulence signalling compound may be a tyrosine-derived cytochalasan compound.

PubMedSearch : Song_2015_Chem.Sci_6_4837
PubMedID: 29142718
Gene_locus related to this paper: phano-phmG , aspcl-CCSE , aspfu-psoB , mago7-ORFZB

Related information

Gene_locus phano-phmG    aspcl-CCSE    aspfu-psoB    mago7-ORFZB

Citations formats

Song Z, Bakeer W, Marshall JW, Yakasai AA, Khalid RM, Collemare J, Skellam E, Tharreau D, Lebrun MH, Lazarus CM, Bailey AM, Simpson TJ, Cox RJ (2015)
Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae
Chem Sci 6 :4837

Song Z, Bakeer W, Marshall JW, Yakasai AA, Khalid RM, Collemare J, Skellam E, Tharreau D, Lebrun MH, Lazarus CM, Bailey AM, Simpson TJ, Cox RJ (2015)
Chem Sci 6 :4837